Identification of dAven, a<i>Drosophila melanogaster</i>ortholog of the cell cycle regulator Aven

Zou, Sige; Chang, Joy; LaFever, Leesa; Tang, Wangli; Johnson, Einer W.; Hu, Jiantao; Wilk, Ronit; Krause, Henry M.; Drummond‐Barbosa, Daniela; Irusta, Pablo M.

doi:10.4161/cc.10.6.15080

Cited by 8 publications

(11 citation statements)

References 81 publications

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“…1 of Ref. [34]). The exception to this is the Drosophila ortholog of Aven (dAven) that was recently identified by Zou and coworkers [34].…”

Section: Resultsmentioning

confidence: 99%

“…[34]). The exception to this is the Drosophila ortholog of Aven (dAven) that was recently identified by Zou and coworkers [34]. Interestingly, the Drosophila protein does not contain a recognizable BH3 motif: the conserved L and D residues as well as other amino acids are not preserved (e.g., an F at position 4a that AVEN shares with BIM and BAD and which makes a significant hydrophobic contribution to the BH3 domain interactions of these proteins; Supplemental File 1) (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Experimental data supports this prediction. In contrast to AVEN, no interaction was found between dAven and Drosophila BCL2 family members [34]. …”

Section: Resultsmentioning

confidence: 99%

“…Numbering using the heptad convention of Dutta and colleagues [11] is shown at the top. Note that the Drosophila protein has diverged considerably from the other proteins and does not contain a putative BH3 motif, but some common residues have been retained within the boxed region (referred to as Region II by Zou and colleagues [34]). The 25-mer AVEN and dAven peptides used in the molecular dynamics simulations of putative BH3 interactions with BCL-xL (Fig.…”

Section: Fig (1)mentioning

confidence: 99%

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An Integrated Bioinformatics and Computational Biology Approach Identifies New BH3-Only Protein Candidates

Hawley

Chen²,

Riz³

et al. 2012

TOBIOJ

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In this study, we utilized an integrated bioinformatics and computational biology approach in search of new BH3-only proteins belonging to the BCL2 family of apoptotic regulators. The BH3 (BCL2 homology 3) domain mediates specific binding interactions among various BCL2 family members. It is composed of an amphipathic α-helical region of approximately 13 residues that has only a few amino acids that are highly conserved across all members. Using a generalized motif, we performed a genome-wide search for novel BH3-containing proteins in the NCBI Consensus Coding Sequence (CCDS) database. In addition to known pro-apoptotic BH3-only proteins, 197 proteins were recovered that satisfied the search criteria. These were categorized according to α-helical content and predictive binding to BCL-xL (encoded by BCL2L1) and MCL-1, two representative anti-apoptotic BCL2 family members, using position-specific scoring matrix models. Notably, the list is enriched for proteins associated with autophagy as well as a broad spectrum of cellular stress responses such as endoplasmic reticulum stress, oxidative stress, antiviral defense, and the DNA damage response. Several potential novel BH3-containing proteins are highlighted. In particular, the analysis strongly suggests that the apoptosis inhibitor and DNA damage response regulator, AVEN, which was originally isolated as a BCL-xL-interacting protein, is a functional BH3-only protein representing a distinct subclass of BCL2 family members.

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“…1 of Ref. [34]). The exception to this is the Drosophila ortholog of Aven (dAven) that was recently identified by Zou and coworkers [34].…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

“…Experimental data supports this prediction. In contrast to AVEN, no interaction was found between dAven and Drosophila BCL2 family members [34]. …”

Section: Resultsmentioning

confidence: 99%

Section: Fig (1)mentioning

confidence: 99%

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An Integrated Bioinformatics and Computational Biology Approach Identifies New BH3-Only Protein Candidates

Hawley

Chen²,

Riz³

et al. 2012

TOBIOJ

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“…While most studies have focused on the abdominal fat body and its role as the analog of the mammalian liver, recent studies have suggested the fat body surrounding the brain functions in housing factors that help to establish the sexual identity of the brain (24). In mammals, it is known that the hypothalamic-pituitary-adrenal axis (HPA), which responds to stress, is influenced by sex hormones such as testosterone and estrogen (55). In one study, researchers found that feminization of the androgen receptor can cause higher HPA Seconds Spent Highly Mobile Fig.…”

Section: Discussionmentioning

confidence: 99%

Altering the sex determination pathway inDrosophilafat body modifies sex-specific stress responses

Argue

Neckameyer

2014

American Journal of Physiology-Regulatory, Integrative and Comp

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Argue KJ, Neckameyer WS. Altering the sex determination pathway in Drosophila fat body modifies sex-specific stress responses. The stress response in Drosophila melanogaster reveals sex differences in behavior, similar to what has been observed in mammals. However, unlike mammals, the sex determination pathway in Drosophila is well established, making this an ideal system to identify factors involved in the modulation of sex-specific responses to stress. In this study, we show that the Drosophila fat body, which has been shown to be important for energy homeostasis and sex determination, is a dynamic tissue that is altered in response to stress in a sex and time-dependent manner. We manipulated the sex determination pathway in the fat body via targeted expression of transformer and transformer-2 and analyzed these animals for changes in their response to stress. In the majority of cases, manipulation of transformer or transformer-2 was able to change the physiological output in response to starvation and oxidative stress to that of the opposite sex. Our data also uncover the possibility of additional downstream targets for transformer and transformer-2 that are separate from the sex determination pathway and can influence behavioral and physiological responses.transformer; transformer-2; behavior; stress; sex differences SEX DIFFERENCES have been observed in the stress response in Drosophila (38,39), analogous to what has been described in mammals. Additional studies have suggested that underlying the different behavioral responses are distinct neuronal stressresponse circuits for males and females (2). These include changes in heart rate and several parameters of locomotor behavior (centrophobism, escape behavior, freezing, and changes in patterns of movement, which are analogous to stress-response behaviors described in mammals), which were assessed after starvation or oxidative stress, two stressors that have been linked to the development of psychiatric illnesses (34,46). It is likely that the mammalian stress response circuitry is also sex specific, based on differences in stress responses and susceptibility to stress-related pathologies (15,45). Drosophila thus provides an ideal model for the elucidation of factors modulating sexual dimorphism in the stress response.We used the genetic tractability of Drosophila to manipulate the sex determination pathway and assess the effect of changing the sexual milieu of the brain on the response to stress. Sex determination is Drosophila is a cell autonomous process, meaning that each cell "decides" whether it will be male or female based on the ratio of X-linked numerator genes [sisterless-a (sisA), sisterless-b (sisB), sisterless-c (sisC), and runt (run)] to an autosomal denominator gene [deadpan (dpn)] (6, 14, 17, 49, 54; reviewed in 44). In females, where the numerator to denominator ratio is 2:2, the resulting gene products are transcription factors that produce functional sex-lethal (SXL) protein (14,17,49,54). In females SXL produces a functional transformer ...

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Mammalian RNA Decay Pathways Are Highly Specialized and Widely Linked to Translation

et al. 2020

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Identification of dAven, aDrosophila melanogasterortholog of the cell cycle regulator Aven

Abstract: Irusta (2011) Identification of dAven, a Drosophilamelanogaster ortholog of the cell cycle regulator Aven, Cell Cycle, 10:6, 989-998,

Cited by 8 publications

References 81 publications

An Integrated Bioinformatics and Computational Biology Approach Identifies New BH3-Only Protein Candidates

An Integrated Bioinformatics and Computational Biology Approach Identifies New BH3-Only Protein Candidates

Altering the sex determination pathway inDrosophilafat body modifies sex-specific stress responses

Mammalian RNA Decay Pathways Are Highly Specialized and Widely Linked to Translation

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