Identification of Differentially Expressed Genes in Semigametic Pima Cotton by Differential Display

Curtiss, Jessica; Turley, Rickie B.; Stewart, James McD.; Zhang, J. F.

doi:10.1007/s11105-011-0378-2

Cited by 7 publications

(2 citation statements)

References 54 publications

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“…One common finding upon analyzing the expression data was the down-regulation of genes associated with the production of transcription and translation factors. Additionally, we were able to find several genes associated with embryo growth and development through mRNA differential display (Curtiss et al, 2012a). A further work developed several molecular markers associated with the semigamy gene (Curtiss et al, 2012b).…”

Section: Semigamymentioning

confidence: 94%

“…Functional Genomics. Different gene expression platforms (including cDNA-AFLP, microarray, RNA-seq, and proteomics) have been used to identify genes/proteins associated with responses to Verticillium wilt infection (Mo et al, 2015); drought and salt stresses (Rodriguez-Uribe et al, 2011; anther, fiber, and boll development (Ma et al, 2012(Ma et al, , 2014(Ma et al, , 2016aPang et al, 2012a,b;Wu et al, 2016); male sterility and restoration (Suzuki et al, 2013a,b;Wei et al, 2013;Zhang et al, 2008a); and semigamy (Curtiss et al, 2011(Curtiss et al, , 2012a.…”

Section: Genomics and Molecular Breedingmentioning

confidence: 99%

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History and Progress in Cotton Breeding, Genetics, and Genomics in New Mexico

Zhang¹

2018

JCS

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The New Mexico Cotton Breeding Program was established in 1926 and has been led by five generations of breeders. The program has released 37 Acala 1517 and one short-staple Upland cotton (Gossypium hirsutum L.) cultivars and numerous germplasm lines. Two Sea-Island G. barbadense L. cultivars have been released for production in the Mesilla Valley, NM. New Mexico germplasm has contributed to the development of 45% of the commercial cotton cultivars including almost all Acala cultivars in California, and has contributed to the improvement in fiber length and strength in U.S. cottons. Many Acala 1517 cultivars are tolerant or resistant to Verticillium wilt and bacterial blight. The recent releases include three transgenic Acala 1517 cultivars, one conventional and two glandless cultivars. The current research program focuses on fiber and seed quality (glandless) to develop elite germplasm with high yields and superior fiber quality and with resistance to Verticillium and Fusarium wilt, thrips, bacterial blight, leaf spot, cotton rust, and tolerance to drought and salinity. Upland × Pima introgression and development of the hybrid seed production system based on cytoplasmic male sterility and the haploid-producing system based on semigamy are also important aspects of the program. Extensive applications of genomic tools and approaches in the program include DNA marker and population development, linkage map construction, and quantitative trait locus mapping. In recent years, reduction in funding and lack of institutional support has hampered the program in delivering solutions to challenging issues such as Fusarium wilt race 4 faced by the cotton farmer.

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Section: Semigamymentioning

confidence: 94%

Section: Genomics and Molecular Breedingmentioning

confidence: 99%

History and Progress in Cotton Breeding, Genetics, and Genomics in New Mexico

Zhang¹

2018

JCS

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Instability in mitochondrial membranes in Polima cytoplasmic male sterility of Brassica rapa ssp. chinensis

Liu

Duan

Song

et al. 2014

Funct Integr Genomics

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Cytoplasmic male sterility (CMS) is an important factor to observe heterosis in Brassica rapa. Although several studies have documented the rearrangements of mitochondrial DNA and dysfunction in the mitochondria have been observed in most types of CMS, the basis of the molecular mechanisms involved in these processes and other effects on CMS remain unclear. In this study, suppression subtractive hybridization was performed in the flowers of an alloplasmic Polima CMS system from B. rapa ssp. chinensis to identify genes that are differentially expressed between fertile and sterile plants. A total of 443 clones were isolated (156 were upregulated in fertile buds, and 287 were upregulated in sterile ones). Real-time RT-PCR further demonstrated the credibility of SSH. Among these genes, many membrane protein genes (LTP12, PIP2A, and GRP14) were inhibited in the sterile male line. Mitochondrial membrane potential (MMP) assay was then performed. Results showed that the sterile MMP was unstable and failed to create a potential difference; thus, mitochondrial dysfunction occurred. Moreover, abnormal microtubules and photosynthetic pathways were found in sterile male cells. Unstable MMP, nutritional deficiency, and abnormal microtubules were the causes of Polima CMS in Brassica campestris. H2O2, MDA, and O(2-), accumulated as byproducts of energy metabolism disorder in sterile male cells.

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