Identification of Differentially Expressed Genes in Brassinosteroid-Treated Brassica napus Seedlings

Dhaubhadel, Sangeeta; Krishna, Priti

doi:10.1007/s00344-008-9056-5

Cited by 20 publications

(11 citation statements)

References 44 publications

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“…Thus, soldat10 is constitutively adapted to light stress [13], whereas a Brassica napus mTERF gene and its Arabidopsis orthologue are up-regulated under abiotic stress conditions [34]. Consistent with this, our analysis using the At-TAX tiling array data showed differences in the expression of most Arabidopsis mTERF genes after salt, mannitol or ABA treatments, suggesting a role for mTERFs in abiotic stress responses.…”

Section: Discussionsupporting

confidence: 84%

Arabidopsis MDA1, a Nuclear-Encoded Protein, Functions in Chloroplast Development and Abiotic Stress Responses

2012

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Most chloroplast and mitochondrial proteins are encoded by nuclear genes, whose functions remain largely unknown because mutant alleles are lacking. A reverse genetics screen for mutations affecting the mitochondrial transcription termination factor (mTERF) family in Arabidopsis thaliana allowed us to identify 75 lines carrying T-DNA insertions. Two of them were homozygous for insertions in the At4g14605 gene, which we dubbed MDA1 (MTERF DEFECTIVE IN Arabidopsis1). The mda1 mutants exhibited altered chloroplast morphology and plant growth, and reduced pigmentation of cotyledons, leaves, stems and sepals. The mda1 mutations enhanced salt and osmotic stress tolerance and altered sugar responses during seedling establishment, possibly as a result of reduced ABA sensitivity. Loss of MDA1 function caused up-regulation of the RpoTp/SCA3 nuclear gene encoding a plastid RNA polymerase and modified the steady-state levels of chloroplast gene transcripts. Double mutant analyses indicated that MDA1 and the previously described mTERF genes SOLDAT10 and RUG2 act in different pathways. Our findings reveal a new role for mTERF proteins in the response to abiotic stress, probably through perturbed ABA retrograde signalling resulting from a disruption in chloroplast homeostasis.

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Section: Discussionsupporting

confidence: 84%

Arabidopsis MDA1, a Nuclear-Encoded Protein, Functions in Chloroplast Development and Abiotic Stress Responses

2012

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“…BL treatment leads to an increase in the expression of genes involved in various physiological responses of various plant species such as Arabidopsis thaliana (Clouse and Sasse 1998), Brassica napus (Dhaubhadel and Krishna 2008), and tomato (Goetz et al 2000). The study of BLmediated gene expression in salt stress conditions will provide insights into how this hormone mediates stress tolerance.…”

Section: Discussionmentioning

confidence: 99%

Root-applied brassinolide can alleviate the NaCl injuries on cotton

et al. 2015

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Brassinolide (BL) is a plant growth regulator. This study analyzes the effects of BL on cotton growth, Na ? accumulation, proline content, MDA content, antioxidant enzyme activities, and differentially expressed genes (DEGs) of cotton roots under NaCl stress. We grew two cotton cultivars, Sumian 12 (salt sensitive) and Sumian 22 (salt tolerant), in continuously aerated Hoagland's nutrient solution supplemented with 200 mM NaCl. NaCl stress increased the Na ? , proline, and MDA content and decreased root activity and protein content in the roots of Sumian 12 and 22, and the change rang of Sumian 12 was higher than that of Sumian 22. The application of BL counteracted the NaCl stress-induced growth inhibition in the two tested cotton cultivars. It reduced the accumulation of Na ? , enhanced proline content, and resulted in an overall change in the activities of antioxidant enzymes causing a decrease in the MDA content of NaCl-stressed roots, and the influence of BL on salt-stressed Sumian 12 plants was more pronounced than that on Sumian 22. The digital gene expression analysis in Sumian 12 indicated that BL application significantly influenced the gene expression in NaCl-stressed roots. The majority of 7659 (3661 up-regulated/3998 down-regulated) DEGs of NaCl/ CK in roots of Sumian 12 were regulated by BL, and the gene expression pattern as a result of the root-applied BL on NaCl-stressed cotton treatment (BL ? NaCl) was similar to the control. Our results indicate that the rootapplied brassinolide alleviates NaCl stress on cotton through improving root activity, physiology, and gene expression.

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“…To identify additional BR-induced gene expression changes in B. napus seedlings, the differential display/reverse transcription polymerase chanin reaction technique was used. Substantial changes were identified in the expression levels of genes encoding a mitochondrial transcription termination factor (mTERF)-related protein, GRP22 (GLYCINE-RICH PROTEIN22), myrosinase, and 3-ketoacyl-CoA thiolase [82]. Transcripts of mTERF-related protein, GRP22, and myrosinase were present at approximately two-, four-, and sixfold higher levels, respectively, in treated seedlings before heat stress, whereas those of 3-ketoacyl-CoA thiolase rose to higher levels in treated seedlings during exposure to heat stress.…”

Section: Systematic Study To Dissect the Role Of Br In Abiotic Stressmentioning

confidence: 99%

Brassinosteroids Confer Stress Tolerance

Divi¹,

Krishna²

2009

Plant Stress Biology

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IntroductionBrassinosteroids (BRs) are a group of plant steroidal hormones that are structurally related to animal and insect steroid hormones. BRs regulate a wide range of physiological responses in plants, including cell elongation, photomorphogenesis, xylem differentiation, seed germination [1], and stress responses [2,3]. Although the growth-promoting properties of BRs were recognized in the early 1970s, the first genetic evidence to suggest that BRs are essential for proper plant development came with the isolation of the BR-deficient mutants det2 (de-etiolated2) and cpd (constitutive photomorphogenic dwarf ) [4,5]. Isolation and sequence analysis of DET2 and CPD genes revealed that the encoded proteins share sequence similarities with steroid 5a-reductases and steroid hydroxylases, respectively, indicating a role for these proteins in steroid metabolism. Indeed, feeding det2 and cpd mutant seedlings with BRs rescued their mutant phenotypes to wild-type in a dose-dependent manner, clearly establishing the roles of DET2 and CPD in BR biosynthesis. Numerous other Arabidopsis BR-deficient and BR-insensitive mutants, displaying phenotypic alterations such as dwarfism, small dark-green leaves, a compact rosette structure, delayed flowering and senescence, and reduced fertility [1], were instrumental in the identification of BR signaling components and in understanding to some extent how BR regulates gene expression [6,7]. Numerous reviews detailing BR effects on plant growth and development and BR signaling mechanisms have surfaced in the recent literature [6-10]; these aspects have therefore been discussed only briefly here. The present chapter is focused on the relatively less explored topic of BR-mediated stress responses in plants [3] and highlights the progress made towards understanding the molecular basis of BRmediated plant stress tolerance.Plant Stress Biology. Edited by H. Hirt BR is perceived at the cell surface by BRI1 (BR-INSENSITIVE1), a plasma membrane-localized leucine-rich repeat (LRR) receptor-like kinase (RLK) [11,12]. BR binding to BRI1 induces a series of biochemical events, such as autophosphorylation of BRI1 in its C-terminal domain, dissociation of an inhibitory protein, BKI1 (BRI1 KINASE INHIBITOR1), and association of BRI1 with another LRR RLK, BAK1 (BRI1-ASSOCIATED RECEPTOR KINASE1) [13,14]. The recent demonstration that BR-dependent activation of BRI1 precedes association with BAK1 has led to a model in which BAK1 enhances signaling output through reciprocal BRI1 transphosphorylation [15]. However, whether the main function of BAK1 is to enhance signaling output of BRI1, promote endocytosis of BRI1, or to help link BRI1 with a downstream signaling protein remains to be determined [7]. Other known components of the BR signaling pathway include GSK3 (GLYCO-GEN SYNTHASE KINASE3), BIN2 (BR-INSENSITIVE2), which negatively regulates the transcription factors BZR1 (BRASSINAZOLE-RESISTANT1) and BES1 (BRI1-EMS SUPPRESSOR1) by phosphorylating them [16,17], while the phosphatase BSU1 (BRI1 SUPPRE...

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Identification of Differentially Expressed Genes in Brassinosteroid-Treated Brassica napus Seedlings

Cited by 20 publications

References 44 publications

Arabidopsis MDA1, a Nuclear-Encoded Protein, Functions in Chloroplast Development and Abiotic Stress Responses

Arabidopsis MDA1, a Nuclear-Encoded Protein, Functions in Chloroplast Development and Abiotic Stress Responses

Root-applied brassinolide can alleviate the NaCl injuries on cotton

Brassinosteroids Confer Stress Tolerance

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