Identification of differentially expressed proteins of Arthrospira (Spirulina) plantensis-YZ under salt-stress conditions by proteomics and qRT-PCR analysis

Wang, Huili; Yang, Yanmei; Chen, Wei; Ding, Li; Li, Peizhen; Zhao, Xiaokai; Wang, Xuedong; Li, Aiying; Bao, Qiyu

doi:10.1186/1477-5956-11-6

Cited by 33 publications

(16 citation statements)

References 37 publications

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“…The selected spots were in-gel digested with trypsin as previously described [ 23 , 24 ] with minor modification. Briefly, protein spots were washed twice with ultrapure water, destained twice with 25mM NH 4 HCO 3 in 50% acetonitrile (ACN), dehydrated with 50% ACN and 100% ACN, and then lyophilized in an ALPHA 2–4 LSC Freeze Dryer (Martin Christ, GmbH, Osterode, Germany).…”

Section: Methodsmentioning

confidence: 99%

Proteome Dynamics and Physiological Responses to Short-Term Salt Stress in Brassica napus Leaves

Jia

Shao

et al. 2015

PLoS ONE

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Salt stress limits plant growth and crop productivity and is an increasing threat to agriculture worldwide. In this study, proteomic and physiological responses of Brassica napus leaves under salt stress were investigated. Seedlings under salt treatment showed growth inhibition and photosynthesis reduction. A comparative proteomic analysis of seedling leaves exposed to 200 mM NaCl for 24 h, 48 h and 72 h was conducted. Forty-four protein spots were differentially accumulated upon NaCl treatment and 42 of them were identified, including several novel salt-responsive proteins. To determine the functional roles of these proteins in salt adaptation, their dynamic changes in abundance were analyzed. The results suggested that the up-accumulated proteins, which were associated with protein metabolism, damage repair and defense response, might contribute to the alleviation of the deleterious effect of salt stress on chlorophyll biosynthesis, photosynthesis, energy synthesis and respiration in Brassica napus leaves. This study will lead to a better understanding of the molecular basis of salt stress adaptation in Brassica napus and provides a basis for genetic engineering of plants with improved salt tolerance in the future.

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Section: Methodsmentioning

confidence: 99%

Proteome Dynamics and Physiological Responses to Short-Term Salt Stress in Brassica napus Leaves

Jia

Shao

et al. 2015

PLoS ONE

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“…Another proteomic study of Arthrospira was focused on protein expression under salt stress (Wang et al, 2013 ). The strain A. platensis -YZ was grown under salt-stress conditions: 0.02, 0.5, 1.0 M NaCl, and the protein expression patterns were compared.…”

Section: Geneticsmentioning

confidence: 99%

Edible Cyanobacterial Genus Arthrospira: Actual State of the Art in Cultivation Methods, Genetics, and Application in Medicine

et al. 2017

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The cyanobacterial genus Arthrospira appears very conserved and has been divided into five main genetic clusters on the basis of molecular taxonomy markers. Genetic studies of seven Arthrospira strains, including genome sequencing, have enabled a better understanding of those photosynthetic prokaryotes. Even though genetic manipulations have not yet been performed with success, many genomic and proteomic features such as stress adaptation, nitrogen fixation, or biofuel production have been characterized. Many of above-mentioned studies aimed to optimize the cultivation conditions. Factors like the light intensity and quality, the nitrogen source, or different modes of growth (auto-, hetero-, or mixotrophic) have been studied in detail. The scaling-up of the biomass production using photobioreactors, either closed or open, was also investigated to increase the production of useful compounds. The richness of nutrients contained in the genus Arthrospira can be used for promising applications in the biomedical domain. Ingredients such as the calcium spirulan, immulina, C-phycocyanin, and γ-linolenic acid (GLA) show a strong biological activity. Recently, its use in the fight against cancer cells was documented in many publications. The health-promoting action of “Spirulina” has been demonstrated in the case of cardiovascular diseases and age-related conditions. Some compounds also have potent immunomodulatory properties, promoting the growth of beneficial gut microflora, acting as antimicrobial and antiviral. Products derived from Arthrospira were shown to successfully replace biomaterial scaffolds in regenerative medicine. Supplementation with the cyanobacterium also improves the health of livestock and quality of the products of animal origin. They were also used in cosmetic preparations.

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“…These genome investigations paved the way for studies of the response of Arthrospira to various stresses using transcriptomic and/or proteomic studies ( e . g ., response to light/dark cycle [ 32 ], temperature stress [ 33 ] or salt stress [ 34 ]). So far, a few studies have investigated the response of Arthrospira to N limitation on the proteomic level [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Trade-Off between Growth and Carbohydrate Accumulation in Nutrient-Limited Arthrospira sp. PCC 8005 Studied by Integrating Transcriptomic and Proteomic Approaches

et al. 2015

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Cyanobacteria have a strong potential for biofuel production due to their ability to accumulate large amounts of carbohydrates. Nitrogen (N) stress can be used to increase the content of carbohydrates in the biomass, but it is expected to reduce biomass productivity. To study this trade-off between carbohydrate accumulation and biomass productivity, we characterized the biomass productivity, biomass composition as well as the transcriptome and proteome of the cyanobacterium Arthrospira sp. PCC 8005 cultured under N-limiting and N-replete conditions. N limitation resulted in a large increase in the carbohydrate content of the biomass (from 14 to 74%) and a decrease in the protein content (from 37 to 10%). Analyses of fatty acids indicated that no lipids were accumulated under N-limited conditions. Nevertheless, it did not affect the biomass productivity of the culture up to five days after N was depleted from the culture medium. Transcriptomic and proteomic analysis indicated that de novo protein synthesis was down-regulated in the N-limited culture. Proteins were degraded and partly converted into carbohydrates through gluconeogenesis. Cellular N derived from protein degradation was recycled through the TCA and GS-GOGAT cycles. In addition, photosynthetic energy production and carbon fixation were both down-regulated, while glycogen synthesis was up-regulated. Our results suggested that N limitation resulted in a redirection of photosynthetic energy from protein synthesis to glycogen synthesis. The fact that glycogen synthesis has a lower energy demand than protein synthesis might explain why Arthrospira is able to achieve a similar biomass productivity under N-limited as under N-replete conditions despite the fact that photosynthetic energy production was impaired by N limitation.

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Identification of differentially expressed proteins of Arthrospira (Spirulina) plantensis-YZ under salt-stress conditions by proteomics and qRT-PCR analysis

Cited by 33 publications

References 37 publications

Proteome Dynamics and Physiological Responses to Short-Term Salt Stress in Brassica napus Leaves

Proteome Dynamics and Physiological Responses to Short-Term Salt Stress in Brassica napus Leaves

Edible Cyanobacterial Genus Arthrospira: Actual State of the Art in Cultivation Methods, Genetics, and Application in Medicine

Trade-Off between Growth and Carbohydrate Accumulation in Nutrient-Limited Arthrospira sp. PCC 8005 Studied by Integrating Transcriptomic and Proteomic Approaches

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