Identification of distinct lipopolysaccharide patterns among Yersinia enterocolitica and Y. enterocolitica-like bacteria

Skurnik, Mikael; Toivonen, Susanna

doi:10.1134/s0006297911070133

Cited by 12 publications

(7 citation statements)

References 45 publications

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“…Compared with P. mirabilis -pAY100.1, P. mirabilis did not express the full O-antigen chain ( Figure 2B ). O-antigen expression by Y. enterocolitica and Y. pseudotuberculosis at 37°C was inhibited ( 75 , 76 ) ( Figure 2A ).…”

Section: Resultsmentioning

confidence: 99%

Proteus mirabilis Targets Atherosclerosis Plaques in Human Coronary Arteries via DC-SIGN (CD209)

Xue

Park

et al. 2021

Front. Immunol.

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Bacterial DNAs are constantly detected in atherosclerotic plaques (APs), suggesting that a combination of chronic infection and inflammation may have roles in AP formation. A series of studies suggested that certain Gram-negative bacteria were able to interact with dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin [DC-SIGN; cluster of differentiation (CD) 209] or langerin (CD207), thereby resulting in deposition of CD209s at infection sites. We wondered if Proteus mirabilis (a member of Proteobacteria family) could interact with APs through CD209/CD207. In this study, we first demonstrated that CD209/CD207 were also receptors for P. mirabilis that mediated adherence and phagocytosis by macrophages. P. mirabilis interacted with fresh and CD209s/CD207-expressing APs cut from human coronary arteries, rather than in healthy and smooth arteries. These interactions were inhibited by addition of a ligand-mimic oligosaccharide and the coverage of the ligand, as well as by anti-CD209 antibody. Finally, the hearts from an atherosclerotic mouse model contained higher numbers of P. mirabilis than that of control mice during infection-challenging. We therefore concluded that the P. mirabilis interacts with APs in human coronary arteries via CD209s/CD207. It may be possible to slow down the progress of atherosclerosis by blocking the interactions between CD209s/CD207 and certain atherosclerosis-involved bacteria with ligand-mimic oligosaccharides.

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Section: Resultsmentioning

confidence: 99%

Proteus mirabilis Targets Atherosclerosis Plaques in Human Coronary Arteries via DC-SIGN (CD209)

Xue

Park

et al. 2021

Front. Immunol.

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“…An aliquot of 10 μl was loaded on the gel and analysed in 12% DOC-PAGE and the LPS bands were visualized by silver staining as described earlier [55]. The DOC-PAGE-based LPS classification of Y. enterocolitica and Y. enterocolitica –like bacteria has been described elsewhere [56]. Briefly, based on the O-polysaccharide (O-PS) the strains are classified into four main LPS types: (i) type A, LPS with homopolymeric O-PS, (ii) type B, LPS with ladder-forming heteropolymeric O-PS, (iii) type C, LPS with single-length O-PS, and (iv) type D, rough or semi-rough LPS without O-PS or with a lipid A core substituted with a single O-repeat unit, respectively.…”

Section: Methodsmentioning

confidence: 99%

Clinical isolates of Yersinia enterocolitica Biotype 1A represent two phylogenetic lineages with differing pathogenicity-related properties

et al. 2012

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BackgroundY. enterocolitica biotype (BT) 1A strains are often isolated from human clinical samples but their contribution to disease has remained a controversial topic. Variation and the population structure among the clinical Y. enterocolitica BT 1A isolates have been poorly characterized. We used multi-locus sequence typing (MLST), 16S rRNA gene sequencing, PCR for ystA and ystB, lipopolysaccharide analysis, phage typing, human serum complement killing assay and analysis of the symptoms of the patients to characterize 298 clinical Y. enterocolitica BT 1A isolates in order to evaluate their relatedness and pathogenic potential.ResultsA subset of 71 BT 1A strains, selected based on their varying LPS patterns, were subjected to detailed genetic analyses. The MLST on seven house-keeping genes (adk, argA, aroA, glnA, gyrB, thrA, trpE) conducted on 43 of the strains discriminated them into 39 MLST-types. By Bayesian analysis of the population structure (BAPS) the strains clustered conclusively into two distinct lineages, i.e. Genetic groups 1 and 2. The strains of Genetic group 1 were more closely related (97% similarity) to the pathogenic bio/serotype 4/O:3 strains than Genetic group 2 strains (95% similarity). Further comparison of the 16S rRNA genes of the BT 1A strains indicated that altogether 17 of the 71 strains belong to Genetic group 2. On the 16S rRNA analysis, these 17 strains were only 98% similar to the previously identified subspecies of Y. enterocolitica. The strains of Genetic group 2 were uniform in their pathogenecity-related properties: they lacked the ystB gene, belonged to the same LPS subtype or were of rough type, were all resistant to the five tested yersiniophages, were largely resistant to serum complement and did not ferment fucose. The 54 strains in Genetic group 1 showed much more variation in these properties. The most commonly detected LPS types were similar to the LPS types of reference strains with serotypes O:6,30 and O:6,31 (37%), O:7,8 (19%) and O:5 (15%).ConclusionsThe results of the present study strengthen the assertion that strains classified as Y. enterocolitica BT 1A represent more than one subspecies. Especially the BT 1A strains in our Genetic group 2 commonly showed resistance to human serum complement killing, which may indicate pathogenic potential for these strains. However, their virulence mechanisms remain unknown.

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“…Y. enterocolitica includes a very heterogeneous group of strains which are classified biochemically to 6 biovars (1A, 1B, 2, 3, 4 and 5). The strains of biovars1B, 2, 3, 4 and 5 are considered pathogenic due to their possession of the virulence plasmid (pYV) and the chromosomal genes ail, ystA, myfA and hreP however the strains that belong to biotype 1A are nonpathogenic because they have not pYV plasmid and the chromosomally encoded genes (Bahgat and Virdi, 2007;Shurnik and Toivonen, 2011;Anna and Jordi, 2012). Serologically, more than 70 serotypes were detected and the most frequent serotypes that cause human infection are O: 3, O: 5, 27, O: 8 and O: 9 (Vazlerova and steinhauserova, 2006).…”

Section: Introductionmentioning

confidence: 99%

Viability of The Isolated Yersinia enterocolitica Strains from Damietta Cheese and Ice Cream at Different Refrigeration and Freezing Temperatures

Thabet

Thabet²

2021

Journal of Applied Veterinary Sciences

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Yersinia enterocolitica is considered one of the most prevalent pathogens transmitted through milk and milk products. Therefore, we aimed to detect the prevalence of these bacteria in cheese and ice cream and study the influence of refrigeration and freezing on its growth patterns. A total of 80 samples of cheese and ice cream were collected from Assiut city, Egypt (40 samples each). The collected samples were examined for the isolation of Y. enterocolitica by the classical culture method and improved by the PCR technique. The incidence of Y. enterocolitica was 17.5 and 25.0 % in the examined cheese and ice cream samples by culture method, respectively, its prevalence in the tested cheese and ice cream samples basing on PCR were 7.5 and 15% since 42.8 and 60.0% of the isolated Y. enterocolotica were confirmed positive. Bio-typing and serotyping of the isolated strains revealed that 8 out of the confirmed strains were pathogenic ; Y. enterocolitica serotype O: 3 was the most prevalent strain, and all of the pathogenic strains carried the virulent ail gene. Nearly similar growth patterns of Y. enterocolitica were recorded during storage of cheese at 4 ± 2C and 30 ± 2ºC and, a significant difference was observed in the 3 rd week, the organism found to be survived for 18 weeks with a mean value of 7.4 ± 0.5 and 6.9 ± 0.16 log cfu/ g for cheese stored at 4 ± 2C and 30 ± 2C respectively. In the case of ice cream, there was a significant difference between the behavior of Y. enterocolitica during freezing storage at-6 ± 2C and-18± 2ºC in the 2 nd week, the mean value for the organism count on the 16 th week was 5.3 ± 0.26 and 5.6 ± 0.39 log cfu/ g, respectively.

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Identification of distinct lipopolysaccharide patterns among Yersinia enterocolitica and Y. enterocolitica-like bacteria

Cited by 12 publications

References 45 publications

Proteus mirabilis Targets Atherosclerosis Plaques in Human Coronary Arteries via DC-SIGN (CD209)

Proteus mirabilis Targets Atherosclerosis Plaques in Human Coronary Arteries via DC-SIGN (CD209)

Clinical isolates of Yersinia enterocolitica Biotype 1A represent two phylogenetic lineages with differing pathogenicity-related properties

Viability of The Isolated Yersinia enterocolitica Strains from Damietta Cheese and Ice Cream at Different Refrigeration and Freezing Temperatures

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