Identification of Dog for Halal Authentification with Gas Chromatography Mass Spectroscopy (GCMS) and Chemometrics

Rahayu, Wiranti Sri; Rohman, Abdul; Sudjadi,; Martono, Sudibyo

doi:10.1166/asl.2018.11940

Cited by 2 publications

(3 citation statements)

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“…The chromatogram data of fatty acid methyl ester content from each Sprague Dawley rats, boars, goats, and cows were grouped using chemometrics PCA with Minitab 19 [25].…”

Section: Discussionmentioning

confidence: 99%

“…In this research, it was added with NaCl, which functions as a precipitate of glycerolate from the breakdown of triglycerides into fatty acid. Sodium in NaCl salt will bind with glycerol and become sodium glycerolate [25]. The supernatant taken is the upper phase since Fatty Acid Methyl Ester (FAME) has the lowest specific gravity compared to other phases.…”

Section: Fat Derivatizationmentioning

confidence: 99%

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Authentication of Sprague Dawley Rats (Rattus Norvegicus) Fat With Gc-MS (Gas Chromatography-Mass Spectrometry) Combined With Chemometrics

Guntarti¹,

Ningrum²,

Gandjar³

et al. 2021

Int J App Pharm

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Objective: Misuse of rat meat as food worried people. Rat meat can come from research waste; one of the rats used in the research was the Sprague Dawley. Analysis of rat meat in food can be done using fat. The aim of this study was to authenticate rat fat with GC-MS combined with chemometrics. Methods: The meat of Sprague Dawley rats, wild boar, goats, cow, and processed meatballs was put in the oven at 90-100 °C for±one hour, then derivatized with BF3 and NaOH in methanol to get the methyl ester for injected in GC-MS instrument. The results obtained were in the form of chromatograms and spectrograms. The data was processed using Principal Component Analysis (PCA) to grouping rat's meat with others (wild boars, goats, cows, and processed meatballs). Results: Rat meat fatty acid analysis results with GC-MS were obtained oleate (43.32±1.43)%, linolenate (32.24±1.46)%, palmitate (19.75±0.09)%, palmitoleate (1.14±0.06)%, stearate (0.26±0.01)%, myristate(0.18±0.01)%, margarate (0.15±0.02)%, and pentadecanoate (0.14±0.01)%. The PCA chemometrics results showed that rat meats had scores that close to cows, which meant they had similar fatty acid composition. Conclusion: The GC-MS method, combined with PCA chemometrics, tested rat fat with other animals and processed meatballs samples on the market.

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“…The chromatogram data of fatty acid methyl ester content from each Sprague Dawley rats, boars, goats, and cows were grouped using chemometrics PCA with Minitab 19 [25].…”

Section: Discussionmentioning

confidence: 99%

Section: Fat Derivatizationmentioning

confidence: 99%

Authentication of Sprague Dawley Rats (Rattus Norvegicus) Fat With Gc-MS (Gas Chromatography-Mass Spectrometry) Combined With Chemometrics

Guntarti¹,

Ningrum²,

Gandjar³

et al. 2021

Int J App Pharm

View full text Add to dashboard Cite

show abstract

“…Analisa GCMS dilakukan untuk mengetahui komposisi masing-masing asam lemak dari sapi, anjing serta hewan lain (ayam, kambing, dan babi) yang diperoleh dari penelitian sebelumnya oleh Rahayu (2017 Berdasarkan tabel 4. kelima sampel lemak yang dianalisa terlihat beberapa perbedaan antara asam lemak hewan satu dengan yang lainnya. Pada lemak anjing muncul beberapa asam lemak yang tidak dimiliki oleh lemak hewan yang lain, diantaranya asam kaproat, asam siklopentanetridekanoat, asam arakhidonat, asam 7,10,13-eikosatrienoat, asam 9,12,15-oktadekatrienoat dengan persentase rendah.…”

Section: Komposisi Asam Lemak Hasil Analisis Gcmsunclassified

Analisis Asam Lemak Daging Anjing pada Bakso Sapi Menggunakan Gas Chromatography Mass Spectrometry (GCMS) yang Dikombinasikan dengan PCA (Principal Component Analysis)

Nugraha

Utami

Rahayu

2018

Indonesia Journal of Halal

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AbstrakPerbedaan harga daging yang tinggi, membuat beberapa penjual yang tidak etis mengganti daging sapi dengan daging anjing secara sengaja untuk mendapatkan keuntungan ekonomis. Penelitian ini bertujuan untuk mengetahui kemampuan GCMS yang dikombinasi dengan kemometrika PCA untuk analisis cepat dari asam lemak anjing pada bakso formulasi dan bakso sapi di pasaran. Metode penelitian adalah non eksperimental berupa identifikasi profil asam lemak daging anjing pada bakso sapi formulasi dan bakso sapi di pasaran. Hasil penelitian yang didapat berupa profil kromatogram GCMS menunjukkan bahwa terdapat perbedaan komposisi asam lemak antara daging anjing dan sapi. Pada lemak daging anjing muncul beberapa asam lemak yang tidak dimiliki oleh lemak daging sapi diantaranya asam kaproat, asam siklopentanetridekanoat, asam arakhidonat, asam 7,10,13-eikosatrienoat, asam 9,12,15-oktadekatrienoat. Analisis kualitatif dari kromatogram GCMS menggunakan PCA menunjukkan lemak sapi, anjing, kambing, babi dan ayam dapat diidentifikasi dan dibedakan. ). The difference of meat prices is high, some unethical seller replaces beef intentionally with dog meat to get economical profits. The study aims to assess the capability of GCMS coupled with chemometrics of PCA for rapid screening of dog fat in beef meatball formulation and beef meatballs in the market. The method research is non experimental that is identification of fatty acid profile of dog meat in beef meatball simulation and beef meatballs in the market. The result obtained from GCMS chromatogram profile showed that there is a difference in fatty acid composition between beef and dog meat. In dog meat fat appears some fatty acids that are not owned by beef fat i.e caproic acid, cyclopentanetridecanoic acid, arachidonate acid, 7,10, 9,12, Abstract Fatty Acid Analysis of Dog Meat in Beef Meatball by Gas Chromatography Mass Spectrometry (GCMS) Combined With PCA (Principle Component Analysis

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Identification of Dog for Halal Authentification with Gas Chromatography Mass Spectroscopy (GCMS) and Chemometrics

Cited by 2 publications

References 0 publications

Authentication of Sprague Dawley Rats (Rattus Norvegicus) Fat With Gc-MS (Gas Chromatography-Mass Spectrometry) Combined With Chemometrics

Authentication of Sprague Dawley Rats (Rattus Norvegicus) Fat With Gc-MS (Gas Chromatography-Mass Spectrometry) Combined With Chemometrics

Analisis Asam Lemak Daging Anjing pada Bakso Sapi Menggunakan Gas Chromatography Mass Spectrometry (GCMS) yang Dikombinasikan dengan PCA (Principal Component Analysis)

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