ABSTRACT

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Kencur (Kaempferia galanga, L.) rhizome contain ethyl-p-methoxycinnamate (EPMS) which has sunscreen properties. Temu kunci (Boesenbergia pandurata (Roxb) Schlecht) rhizome contains flavonoid and essential oils with radical scavenging properties. The aims of this study were to obtain the optimum physical properties of sunscreen and antioxidant cream and to compare the sun protection factor (SPF) values and antioxidant activity of kencur and temu kunci rhizomes extract before and after formulated. This research used Simplex Latice Design (SLD) model with 2 components of kencur extract and temu kunci extract. Based on the SLD model obtained optimum formula design, then the SPF values and antioxidant activity were studied by UV spectrophotometric method and DPPH method respectively. Based on SLD model obtained optimum formula that containing kencur : temu kunci extract 80%:20% (formula A) and 70%:30% (formula B). The SPF values of kencur extract, formula A and formula B were 4.505, 5.024 and 4.511 respectively. Antioxidant activity showed that the IC 50 of formula A, formula B, temu kunci extract, BHT and Vitamin E were 109.15 µg/mL, 95.23 µg/mL, 10.20 µg/mL, 22.33 µg/mL and 8.78 µg/mL respectively. Cream formula A and formula B have optimum physical properties. The SPF value of the optimum formulations were higher than SPF value of kencur extract. Antioxidant activity of that combinations were lower than temu kunci extract, BHT and Vitamin E.

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AbstrakPerbedaan harga daging yang tinggi, membuat beberapa penjual yang tidak etis mengganti daging sapi dengan daging anjing secara sengaja untuk mendapatkan keuntungan ekonomis. Penelitian ini bertujuan untuk mengetahui kemampuan GCMS yang dikombinasi dengan kemometrika PCA untuk analisis cepat dari asam lemak anjing pada bakso formulasi dan bakso sapi di pasaran. Metode penelitian adalah non eksperimental berupa identifikasi profil asam lemak daging anjing pada bakso sapi formulasi dan bakso sapi di pasaran. Hasil penelitian yang didapat berupa profil kromatogram GCMS menunjukkan bahwa terdapat perbedaan komposisi asam lemak antara daging anjing dan sapi. Pada lemak daging anjing muncul beberapa asam lemak yang tidak dimiliki oleh lemak daging sapi diantaranya asam kaproat, asam siklopentanetridekanoat, asam arakhidonat, asam 7,10,13-eikosatrienoat, asam 9,12,15-oktadekatrienoat. Analisis kualitatif dari kromatogram GCMS menggunakan PCA menunjukkan lemak sapi, anjing, kambing, babi dan ayam dapat diidentifikasi dan dibedakan. ). The difference of meat prices is high, some unethical seller replaces beef intentionally with dog meat to get economical profits. The study aims to assess the capability of GCMS coupled with chemometrics of PCA for rapid screening of dog fat in beef meatball formulation and beef meatballs in the market. The method research is non experimental that is identification of fatty acid profile of dog meat in beef meatball simulation and beef meatballs in the market. The result obtained from GCMS chromatogram profile showed that there is a difference in fatty acid composition between beef and dog meat. In dog meat fat appears some fatty acids that are not owned by beef fat i.e caproic acid, cyclopentanetridecanoic acid, arachidonate acid, 7,10, 9,12, Abstract Fatty Acid Analysis of Dog Meat in Beef Meatball by Gas Chromatography Mass Spectrometry (GCMS) Combined With PCA (Principle Component Analysis