Identification of economically important <i>Liriomyza</i> species by PCR‐RFLP analysis*

Kox, L. F. F.; Beld, H. E. van den; Lindhout, B. I.; Goffau, L. J. W. de

doi:10.1111/j.1365-2338.2005.00807.x

Cited by 19 publications

(18 citation statements)

References 10 publications

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“…All Liriomyza leafminer species were maintained at 27 ± 2 ºC with a photoperiod of 12L : 12D and 30% R.H. Species were identified using PCR‐restriction fragment length polymorphism (PCR‐RFLP) adapted from Scheffer and Lewis (2001, 2005) and Kox et al. (2005) with assistance from B. Wagener ( icipe ).…”

Section: Methodsmentioning

confidence: 99%

Host plant effects on morphometric characteristics of Liriomyza huidobrensis, L. sativae and L. trifolii (Diptera: Agromyzidae)

Musundire

Chabi-Olaye

Krüger

2010

J Applied Entomology

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Body size of herbivorous insects may be directly related to host plant characteristics (e.g. nutrition, chemical composition) and positively linked to performance of the herbivore and its parasitoids. Differences in adult body size of Liriomyza huidobrensis, L. sativae and L. trifolii reared on Pisum sativum, Phaseolus vulgaris, Vicia faba and Solanum lycopersicum were assessed to determine the effect of host plants on adult size. Furthermore, morphometric wing and hind tibia measurements were used to determine suitability as proxy measures for adult size. Based on canonical and linear discriminant analyses, two distinct Liriomyza morphospecies groups were recognized across all plant species. The first cluster represented L. huidobrensis and the second L. sativae and L. trifolii. The overall degree of correct classification of the three species across all plants tested (correct observation) with cross validation was estimated at 85%. Liriomyza huidobrensis, the largest species, showed no differences in the variables measured when reared on the four plant species. However, different plant species seem to influence wing and hind tibia measurements in L. trifolii to a greater extent than in L. huidobrensis and L. sativae. The first two canonical variates could not separate the populations of L. huidobrensis and L. sativae reared on different plant species, but showed separation of L. trifolii populations into two clusters: firstly, insects reared on V. faba and secondly, insects reared on P. vulgaris and S. lycopersicum. Hind tibia length strongly correlated with wing length. Both can be used to determine adult size of the three Liriomyza species. This study provides some new evidence for the existence of differential preferences by Liriomyza species for the four host plant species. This, therefore, warrants larger scale field studies to test for positive preference‐performance of Liriomyza species on these plant species and to determine possible consequences at the third tritrophic level.

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Section: Methodsmentioning

confidence: 99%

Host plant effects on morphometric characteristics of Liriomyza huidobrensis, L. sativae and L. trifolii (Diptera: Agromyzidae)

Musundire

Chabi-Olaye

Krüger

2010

J Applied Entomology

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“…However, proteins are not stable and subject to environmental influences, and the method is less sensitive than DNA methods (Hoy ; Michael et al ). DNA techniques have been widely used for the detection of Liriomyza species, for example, the random amplified polymorphic DNA (RAPD)‐PCR techniques (Chiu et al ; Morgan et al ) and PCR‐restriction fragment length polymorphism (PCR‐RFLP) method (Scheffer et al ; Kox et al ). Out of the two methods, RAPD‐PCR can only be used for very close comparisons and must be used cautiously because of the increased chance of non‐homology of co‐migrating fragments (Bowditch et al ).…”

Section: Introductionmentioning

confidence: 99%

Multiplex real‐time PCR assay for the detection of three invasive leafminer species: Liriomyza huidobrensis, L. sativae and L. trifolii (Diptera: Agromyzidae)

Sooda

Gunawardana

et al. 2016

Austral Entomology

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Rapid and precise identification of immature stages of leafminers of the genus Liriomyza Mik associated with imported fresh produce is essential to ensure appropriate biosecurity decisions at the border, in quarantine and post border. The leafminers Liriomyza huidobrensis, Liriomyza sativae and Liriomyza trifolii are not present in New Zealand and classified as regulated pests when detected at New Zealand's border. To assist rapid species identification of the immature stages of interceptions, a multiplex real-time TaqMan PCR assay was developed to identify these three species simultaneously in a single test. Species-specific primers and probes were designed by amplifying the mitochondrial COI gene of each targeting species, respectively. The multiplex real-time PCR assay demonstrated high specificity for all three target species and the assay detected DNA quantities as low as 0.1 pg for all species. Linear responses and high correlation coefficients between the amount of DNA and C q values for each species were also achieved. Therefore, the assay demonstrated its sensitivity and reliability for the identification of these three invasive Liriomyza species.

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“…Amplification of a 790-bp fragment of mitochondrial cytochrome oxidase II (COII) DNA followed by restriction fragment length polymorphism (RFLP) analysis was developed by Kox et al (2005). RFLPbased method is a powerful diagnostic tool for reliable identification of all life stages of economically important Liriomyza species, but this method is somewhat time-consuming, needing post-amplification digestion and requiring the time for diagnosis (Fettene and Temu 2003;Kampen et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Molecular identification of Liriomyza trifolii (Burgess) (Dipt., Agromyzidae) based on real‐time PCR

Feng

Chen

et al. 2007

J Applied Entomology

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Rapidly identifying juvenile individuals of Liriomyza trifolii (Burgess) from Liriomyza sativae Blanchard is crucial in plant quarantine. We report a molecular method to identify L. trifolii based on real-time polymerase chain reaction (PCR). By comparing partial DNA sequences of mitochondrial COI genes of L. trifolii samples collected from Guangdong and Taiwan provinces in China, Japan, Philippine, Israel, Germany, the USA, Mexico and Honduras sequenced by authors, and those of related species recorded in GenBank, a L. trifolii-specific probe was developed. There was no difference in individuals of different stages tested by this probe. The total time for real-time PCR assay system was 2 h, and it would save 3-7 h compared with conventional PCR.

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Identification of economically important Liriomyza species by PCR‐RFLP analysis*

Cited by 19 publications

References 10 publications

Host plant effects on morphometric characteristics of Liriomyza huidobrensis, L. sativae and L. trifolii (Diptera: Agromyzidae)

Host plant effects on morphometric characteristics of Liriomyza huidobrensis, L. sativae and L. trifolii (Diptera: Agromyzidae)

Multiplex real‐time PCR assay for the detection of three invasive leafminer species: Liriomyza huidobrensis, L. sativae and L. trifolii (Diptera: Agromyzidae)

Molecular identification of Liriomyza trifolii (Burgess) (Dipt., Agromyzidae) based on real‐time PCR

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