Identification of ephedrines as their carbon disulfide derivatives

Merwe, P. J. van der; Hendrikz, S.E.

doi:10.1016/0378-4347(94)00429-9

Cited by 22 publications

(8 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Ephedrine (EP), pseudoephedrine (c-EP), norephedrine (Nor-EP) and N-methylephedrine (Me-c-EP) are pairs of diastereoisomeric sympathomimetic amines that act directly and indirectly on the sympathetic nerves [1,2]. Ephedracontaining products, also known as ephedra alkaloids, are usually marketed and labeled as energy-boosters, weightloss aids and athletic performance enhancers.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous chiral separation and determination of ephedrine alkaloids by MEKC‐ESI‐MS using polymeric surfactant I: Method development

et al. 2007

View full text Add to dashboard Cite

In this work, simultaneous separation of eight stereoisomers of ephedrine and related compounds ((+/-)-ephedrine, (+/-)-pseudoephedrine, (+/-)-norephedrine and (+/-)-N-methylephedrine) was accomplished using a polymeric chiral surfactant, i.e. polysodium N-undecenoxycarbonyl-L-leucinate (poly-L-SUCL) by chiral (C)MEKC-ESI-MS. The conditions of CMEKC were first investigated. The baseline separation of all eight stereoisomers of ephedrine and related compounds was achieved under optimum CMEKC conditions (35 mM poly-L-SUCL, 15 mM NH(4)OAc, pH 6.0, 30% v/v ACN, 30 kV and 20 degrees C) in less than 30 min. Next, a central composite design for response surface modeling has been described to evaluate the electrospray chamber parameters and the sheath liquid conditions. Optimum mass abundance of stereoisomers of ephedrine and related compounds was observed using the spray chamber parameters, namely 250 degrees C drying gas temperature and 8 L/min drying gas flow rate at a nebulizer pressure of 4 psi. Furthermore, the experimental design indicates that the optimum mass abundance of the stereoisomers of ephedrine and related compounds can be obtained using a sheath liquid containing 80:20 v/v methanol-water, 5 mM NH(4)OAc at pH 8.5 delivered at 5 microL/min. Finally, compared to MEKC-UV, the use of poly-L-SUCL in MEKC-MS provided significantly higher sensitivity for stereoisomers of ephedrine and related compounds.

show abstract

Section: Introductionmentioning

confidence: 99%

Simultaneous chiral separation and determination of ephedrine alkaloids by MEKC‐ESI‐MS using polymeric surfactant I: Method development

et al. 2007

View full text Add to dashboard Cite

show abstract

“…In forensic analyses, ephedrines are potentially interesting, mainly in investigations of accidents involving intoxications [3,4]. Analysis of ephedrines has been a classical procedure in doping control since the 1960s, using gas chromatography (GC) as the preferred technique [5,6]. Since these compounds are diastereoisomers, the characterization based on mass spectral interpretation is not conclusive for identification purposes.…”

Section: Introductionmentioning

confidence: 99%

Consequences of the formation of 3,4-dimethyl-5-phenyl-1,3-oxazolidine on the analysis of ephedrines in urine by gas chromatography and a new method for confirmation as N-trifluoroacetyl-O-t-butyldimethylsilyl ether derivatives

Sardela

Pereira

et al. 2011

Journal of Chromatography A

View full text Add to dashboard Cite

“…1,2 These substances are ingredients of Ma Huang, which is a traditional Chinese medicine (TCM) derived from ephedrae herba that is used as a stimulant, diaphoretic, and anti-asthmatic. 3 They are also ingredients of many pharmaceuticals commonly used in the treatment of flu, rhinitis, colds, and allergy.…”

Section: Introductionmentioning

confidence: 99%

Separation and determination of ephedra alkaloids in traditional Chinese medicine and human urines by capillary electrophoresis coupled with electrochemiluminescence detection

Liu

Yang

et al. 2012

Can. J. Chem.

View full text Add to dashboard Cite

A new approach for the simultaneous determination of ephedrine, methylephedrine, and pseudoephedrine is developed, using capillary electrophoresis coupled with electrochemiluminescence detection with internal standard method. Separation efficiency and sensitivity were improved by use of an ionic liquid. Parameters affecting separation and detection were investigated in detail. Under optimum conditions, the three ephedra alkaloids were well separated and detected. The limits of detection (S/N = 3) of ephedrine, methylephedrine and pseudoephedrine are 4.0 × 10 -8 , 6.5 × 10 -8 , and 4.6 × 10 -8 mol/L, respectively. The limits of quantitation (S/N = 10) in human urine are 5.3 × 10 -7 mol/L for ephedrine, 9.1 × 10 -7 mol/L for methylephedrine, and 6.9 × 10 -7 mol/L for pseudoephedrine, respectively. The precision (RSD%) of the peak area and the migration time were from 2.2% to 2.5% and from 0.1% to 0.2% within a day and from 2.7% to 3.9% and from 0.4% to 0.8% in three days. The proposed method was successfully applied to the determination of three analytes in traditional Chinese medicine and human urine, and the monitoring of pharmacokinetics of pseudoephedrine in human body.Résumé : On a développé une nouvelle méthode de détermination simultanée de l'éphédrine, de la méthyléphédrine et de la pseudoéphédrine par électrophorèse capillaire couplée à un détecteur à électrochimiluminescence, utilisant un standard interne. On a amélioré l'efficacité et la sensibilité de la séparation en utilisant un liquide ionique. On a étudié en détail les paramètres qui peuvent affecter la séparation et la détection. Dans des conditions optimales, les alcaloïdes de l'éphédra sont bien séparés et bien détectés. Les limites de détection (signal/bruit > 3) de l'éphédrine, de la méthyléphédrine et de la pseudoéphédrine sont respectivement de 4,0 × 1 -8 , 6,5 × 1 -8 et 4,6 × 1 -8 mol/L. Les limites de quantification (signal/bruit > 10) dans l'urine humaine sont pour l'éphédrine, la méthyléphédrine et la pseudoéphédrine sont respectivement de 5,3 × 10 -7 , 9,1 × 10 -7 et 6,9 × 10 -7 mol/L. Les précisions, écarts types relatifs (ETR%), de la surface sous les pics et des temps de migration varient de 2,2 % à 2,5 % et de 0,1 % à 0,2 % au cours d'une journée et de 2, 7 % à 3,9 % et de 0,4 % à 0,8 % en trois jours. La méthode proposée a été appliquée avec succès à la détermination des trois analytes dans des médicaments chinois traditionnels, dans l'urine humaine et au suivi pharmacocinétique de la pseudoéphédrine dans le corps humain.

show abstract

Identification of ephedrines as their carbon disulfide derivatives

Cited by 22 publications

References 8 publications

Simultaneous chiral separation and determination of ephedrine alkaloids by MEKC‐ESI‐MS using polymeric surfactant I: Method development

Simultaneous chiral separation and determination of ephedrine alkaloids by MEKC‐ESI‐MS using polymeric surfactant I: Method development

Consequences of the formation of 3,4-dimethyl-5-phenyl-1,3-oxazolidine on the analysis of ephedrines in urine by gas chromatography and a new method for confirmation as N-trifluoroacetyl-O-t-butyldimethylsilyl ether derivatives

Separation and determination of ephedra alkaloids in traditional Chinese medicine and human urines by capillary electrophoresis coupled with electrochemiluminescence detection

Contact Info

Product

Resources

About