Identification of fatty acid binding proteins as markers associated with the initiation and/or progression of renal cell carcinoma

Seliger, Barbara; Lichtenfels, Rudolf; Atkins, Derek; Bukur, Jürgen; Halder, Thomas; Kersten, Michael; Harder, Alois; Ackermann, Andreas; Malenica, B; Brenner, Walburgis; Zobawa, Monica; Lottspeich, Friedrich

doi:10.1002/pmic.200401264

Cited by 34 publications

(45 citation statements)

References 43 publications

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“…Of these, 24 proteins have been described previously [16][17][18] and almost all of the presently obtained results were consistent with the earlier observations. For instance, our finding of higher levels of proteins involved in glycolysis (such as glyceraldehyde-3-phosphate dehydrogenase and fructose-bisphosphate aldolase A) was consistent with earlier reports [16][17][18][19][20][21].…”

Section: Discussionsupporting

confidence: 93%

Quantitative proteomic analysis to discover potential diagnostic markers and therapeutic targets in human renal cell carcinoma

et al. 2008

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Renal cell carcinoma (RCC) is relatively resistant to chemotherapy and radiotherapy. Recent advances in drug development are providing novel agents for the treatment of RCC, but the effects are still minimal. In addition, there is an urgent need to identify diagnostic markers for RCC. In this report, to discover potential diagnostic markers and therapeutic targets, we subjected RCC samples to a quantitative proteomic analysis utilizing 2-nitrobenzenesulfenyl (NBS) reagent. Proteins were extracted from RCC and adjacent normal tissue, obtained surgically from patients, and labeled with NBS reagent containing six 12 C or 13 C. This was followed by trypsin digestion and the enrichment of labeled peptides. Samples were then subjected to analysis by MALDI-TOF MS. NBS-labeled peptides with a 6 Da difference were identified by MS/MS. Thirtyfour proteins were upregulated in more than 60% of the patients of which some were previously known, and some were novel. The identity of a few proteins was confirmed by Western blotting and quantitative real time RT-PCR. The results suggest that NBS-based quantitative proteomic analysis is useful for discovering diagnostic markers and therapeutic targets for RCC.

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Section: Discussionsupporting

confidence: 93%

Quantitative proteomic analysis to discover potential diagnostic markers and therapeutic targets in human renal cell carcinoma

et al. 2008

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“…Five selected chimeric transcript predictions were confirmed in DLBCL cell lines and we conducted further analysis of one particular chimera, namely LTR2-FABP7, which we confirmed encodes a novel isoform of the FABP7 protein with an alternative first coding exon. FABP7 is normally expressed in brain, but not in blood, and is up-regulated in various solid tumor types, where it is a marker for poor prognosis (28)(29)(30)(31). However, it has not been associated with DLBCL or any other lymphoma before this report.…”

mentioning

confidence: 74%

“…Although FABP7 expression has not been linked with blood cancers before, up-regulation has been reported in various solid cancer types, including malignant glioma (28), melanoma (31), renal cell carcinoma (30), and aggressive triple negative breast cancer (47) and is associated with poor prognosis and also increased cell growth in vitro (48). To assess the requirement for chimeric FABP7 in DLBCL proliferation and cell-cycle progression in vitro, DLBCL cell lines were nucleofected to express three independent RNAi sequences targeting FABP7 and a nonsilencing scrambled control sequence.…”

Section: Activation Of Te-gene Chimeras Is Associated With Epigeneticmentioning

confidence: 99%

Distinct isoform of FABP7 revealed by screening for retroelement-activated genes in diffuse large B-cell lymphoma

Lock

Rebollo

Miceli-Royer

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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Significance Sequences derived from transposable elements (TEs) are abundant in the human genome and can influence gene expression. In normal cells, most TEs are silenced by epigenetic mechanisms such as DNA methylation but, in cancer, normally dormant TEs can become active. We hypothesized that cancer-specific release of epigenetic suppression of TEs could result in gene expression perturbations, which could promote oncogenesis. Using a bioinformatics method, we identified many genes expressed in diffuse large B-cell lymphoma (DLBCL) via activation of TE promoters. Further analysis of one, FABP7 , showed it was expressed in some DLBCL samples through use of a TE promoter. The TE-driven FABP7 transcript encodes a novel isoform of the protein, which is required for optimal DLBCL cell line proliferation.

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“…previous studies revealed that B-FABp was overexpressed in renal cell carcinoma tissues (7,12,13). the present study proves that B-FABP influences the proliferation and migration in renal cell carcinoma cell lines whereas the invasion ability of the cells did not depend on their B-FABp content.…”

Section: Discussionmentioning

confidence: 89%

Importance of brain‑type fatty acid binding protein for cell-biological processes in human renal carcinoma cells

Tölle

Krause²,

Miller³

et al. 2011

Oncol Rep

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Abstract. the molecular mechanisms underlying renal cell carcinoma (rcc) development and progression are still not completely understood. the importance of fatty acid binding proteins (FABp) for the progression of carcinomas has been shown for several tumors. However, the importance of braintype FABp (B-FABp) in cell-biological processes in renal carcinoma cells is unknown. therefore, it was the aim of this study to evaluate the role of B-FABp in processes such as proliferation, migration and invasion. By using the approach of down-and up-regulation of B-FABp in human kidney carcinoma cells caki-2 and caki-1, the potential participation of B-FABp in proliferation, migration and invasion was demonstrated. B-FABp was down-regulated at both mrnA and protein levels following treatment of caki-2 cells with B-FABp sirnA. Down-regulation of B-FABp decreased cell proliferation and migration but did not affect invasion. the transfection of caki-1 cells with human B-FABp cDnA generated an increment of B-FABp mrnA but the protein was not detectable. transfected caki-1 cells developed a faster proliferation compared to untreated cells. An effect on the process of invasion was not observed. our data suggest that B-FABp is involved in cell proliferation and migration of human renal carcinoma cells. the detailed molecular mechanisms remain to be elucidated.

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Identification of fatty acid binding proteins as markers associated with the initiation and/or progression of renal cell carcinoma

Cited by 34 publications

References 43 publications

Quantitative proteomic analysis to discover potential diagnostic markers and therapeutic targets in human renal cell carcinoma

Quantitative proteomic analysis to discover potential diagnostic markers and therapeutic targets in human renal cell carcinoma

Distinct isoform of FABP7 revealed by screening for retroelement-activated genes in diffuse large B-cell lymphoma

Importance of brain‑type fatty acid binding protein for cell-biological processes in human renal carcinoma cells

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