Identification of female sex pheromone of a plant bug, <i>Adelphocoris fasciaticollis</i> Reuter (Hemiptera: Miridae)

Zhang, T.; Mei, Xiangdong; Zhang, L.; Wu, Kongming; Ning, Jun

doi:10.1111/jen.12153

Cited by 15 publications

(25 citation statements)

References 22 publications

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“…The responses of siRNA‐injected and water‐injected A. lineolatus to these six compounds were investigated by EAG assays. Tridecanal and hexyl alcohol are plant volatiles released by several host plants of miridae bugs (Chinta et al ., ; Gu et al ., ), and butyl butanoate, 1‐hexyl butyrate, ( E )‐2‐hexenyl butyrate and hexyl hexanoate are putative sex pheromone components in miridae bugs (Zhang et al ., ; Zhang et al ., ). EAG values were reduced in RNAi‐treated bug antennae compared with controls.…”

Section: Resultsmentioning

confidence: 97%

Silencing of odorant binding protein gene AlinOBP4 by RNAi induces declining electrophysiological responses of Adelphocoris lineolatus to six semiochemicals

Zhang,

Zhu,

et al. 2016

Insect Science

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Odorant binding proteins (OBPs) are believed to be important for transporting semiochemicals through the aqueous sensillar lymph to the olfactory receptor cells within the insect antennal sensilla. Here, we injected AlinOBP4-siRNA into the conjunctivum between prothorax and mesothorax of the lucerne plant bug, Adelphocoris lineolatus and evaluated the silencing of AlinOBP4 by reverse transcription polymerase chain reaction (RT-PCR) analysis, quantitative real-time PCR (qPCR) test and electroantennogram (EAG) assay. The combination of RT-PCR and qPCR analyses revealed that the levels of messenger RNA transcript were significantly reduced ∼95% in AlinOBP4-siRNA-treated A. lineolatus males and ∼75% in RNAi-treated females within 48 hours. It was found that there are different EAG responses between male and female bugs when the AlinOBP4 gene was silenced by RNAi. The EAGs of A. lineolatus to two plant volatiles, tridecanal and hexyl alcohol, were reduced 9.09% and 79.45% in RNAi-treated males, 62.08% and 62.08% in RNAi-treated females compared to the controls, separately. Antennae of RNAi-treated bugs showed significantly lower electrophysiological responses to four sex pheromone analogs, butyl butanoate, 1-hexyl butyrate, (E)-2-hexenyl butyrate and hexyl hexanoate. The EAG recordings were reduced 35.43%, 35.24%, 39.96% and 78.47% in RNAi-treated males and 64.52%, 18.13%, 36.88% and 49.52% in RNAi-treated females, respectively. The results suggested that AlinOBP4 might play dual-roles in the identification of plant volatiles and sex pheromones. It was suspected that AlinOBP4 may have different functions in odor perception between male and female A. lineolatus.

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Section: Resultsmentioning

confidence: 97%

Silencing of odorant binding protein gene AlinOBP4 by RNAi induces declining electrophysiological responses of Adelphocoris lineolatus to six semiochemicals

Zhang,

Zhu,

et al. 2016

Insect Science

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“…Previously, we used septa as dispensers for the pheromone of A . fasciaticollis , but the lures lost attractiveness after 3 days. Novel dispensers for long‐term use with mirid pheromones have been developed more recently.…”

Section: Discussionmentioning

confidence: 93%

“…fasciaticollis , Ad . suturalis and Halticus minutus . Almost all of the sex pheromone components that have been published for mirid species were blends of acetate, butyrate, hexanoate and 4‐OHE (Table S1).…”

Section: Discussionmentioning

confidence: 99%

“…Pheromone was collected by whole‐body extraction, as described previously . Briefly, at 1–2 h into the scotophase, a virgin adult (3‐day‐old) or fifth‐instar nymph of A .…”

Section: Methodsmentioning

confidence: 99%

“…To measure the release rates of the pheromone components, lures containing 4‐OHE and E2HB (9 + 6 mg, respectively) in 200 μL of sunflower oil were exposed to a laboratory wind tunnel (27 °C, 8 km h −1 wind speed). At regular intervals, the volatiles emitted from the lures were collected using the head space method . Briefly, individual lures were placed in glass aeration chambers (30 cm long × 3.5 cm diameter), and volatiles were collected for 1 h. Humidified, charcoal‐filtered air was pushed into the chambers at a flow rate of 200 mL min −1 .…”

Section: Methodsmentioning

confidence: 99%

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Identification and field evaluation of the sex pheromone of Apolygus lucorum (Hemiptera: Miridae) in China

Tao

Mei

Zhang

et al. 2020

Pest Management Science

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BACKGROUND The plant bug, Apolygus lucorum Meyer‐Dür, has begun a resurgence and has become a key pest in cotton in northern China, with the wide‐scale adoption of transgenic Bt cotton. We attempted to develop a new approach to the control of this plant bug by identifying and utilizing its sex pheromone. RESULTS Extracts from A. lucorum adults and nymphs were analyzed using gas chromatography–mass spectrometry (GC–MS) and gas chromatography–electroantennogram detection (GC–EAD) to identify the sex pheromone components. (E)‐4‐Oxo‐2‐hexenal and (E)‐2‐hexenyl butyrate were the major sex pheromone components from females and were also electrophysiology‐active towards male antennae. For males, (E)‐4‐oxo‐2‐hexenal and hexyl butyrate were the major sex pheromone components. These three components, however, were not detected in nymphs. Field tests showed that binary blends of (E)‐4‐oxo‐2‐hexenal and (E)‐2‐hexenyl butyrate at a ratio of 3:2 resulted in trapping the greatest numbers of A. lucorum males. A long‐term dispenser was developed by loading the pheromone components dissolved in sunflower oil into polyethylene vials. Further field evaluation showed that the polyethylene dispensers attracted significantly more bugs even when deployed in the field for 5 weeks. CONCLUSION We identified the sex pheromone of A. lucorum and developed a type of high‐efficiency and long‐term lure. Our results could expand knowledge of the pheromones of plant bugs, and provide novel technologies to monitor and control this pest. © 2019 Society of Chemical Industry

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Optimization and field demonstration of the Lygus pratensis (Hemiptera: Miridae) sex pheromone

Zhang

Wyckhuys

et al. 2020

Pest Management Science

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BACKGROUND The plant bug Lygus pratensis Linnaeus is a widely distributed polyphagous herbivore that increasingly attains outbreak population levels on cotton in northwestern China. Although the sex pheromone of L. pratensis from the United Kingdom has been identified as hexyl butyrate, (E)‐2‐hexenyl butyrate and (E)‐4‐oxo‐2‐hexenal, at a ratio of 100:25:24, this volatile blend does not prove attractive to Chinese field populations. RESULTS In this study, we identified and optimized the sex pheromone of L. pratensis strains from northwestern China. In coupled gas chromatography and electro‐antennogram detection (GC–EAD) assays, three compounds within whole‐body extracts of virgin L. pratensis females elicited antennal responses: hexyl butyrate, (E)‐2‐hexenyl butyrate and (E)‐4‐oxo‐2‐hexenal. In field trials, a 20:1:30 ratio blend was the most attractive to L. pratensis males. CONCLUSION Traps baited with this synthetic pheromone blend present considerable advantages over traditional sweep‐net sampling for L. pratensis population monitoring. It can readily be incorporated into monitoring schemes and integrated pest management packages.

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Identification of female sex pheromone of a plant bug, Adelphocoris fasciaticollis Reuter (Hemiptera: Miridae)

Cited by 15 publications

References 22 publications

Silencing of odorant binding protein gene AlinOBP4 by RNAi induces declining electrophysiological responses of Adelphocoris lineolatus to six semiochemicals

Silencing of odorant binding protein gene AlinOBP4 by RNAi induces declining electrophysiological responses of Adelphocoris lineolatus to six semiochemicals

Identification and field evaluation of the sex pheromone of Apolygus lucorum (Hemiptera: Miridae) in China

Optimization and field demonstration of the Lygus pratensis (Hemiptera: Miridae) sex pheromone

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