Identification of five Mitragyna alkaloids in urine using liquid chromatography-quadrupole/time of flight mass spectrometry

Basiliere, Stephanie; Bryand, Kelsie; Kerrigan, Sarah

doi:10.1016/j.jchromb.2018.02.010

Cited by 29 publications

(17 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Lee et al [ 121 ] developed an analytical method in LC-MS/MS, where they used SPE and enzymatic hydrolysis as a method of pre-treatment of the urine sample, to quantify 16-carboxy mitragynine, 9- O -demethyl mitragynine and mitragynine. More recently, Basiliere et al [ 122 ] developed an analytical method using LC-Q/TOF-MS equipment for the quantification of mitragynine, 7-hydroxymitragynine, among other compounds. One milliliter of urine, pre-treated with SPE, was used, obtaining a LOD of 0.25–1 ng/mL and a LOQ of 0.5–1 ng/mL [ 122 ].…”

Section: Mitragyna Speciosa (Kratom)mentioning

confidence: 99%

See 1 more Smart Citation

Psychoactive Substances of Natural Origin: Toxicological Aspects, Therapeutic Properties and Analysis in Biological Samples

et al. 2021

View full text Add to dashboard Cite

The consumption of new psychoactive substances (NPSs) has been increasing, and this problem affects several countries worldwide. There is a class of NPSs of natural origin, consisting of plants and fungi, which have a wide range of alkaloids, responsible for causing relaxing, stimulating or hallucinogenic effects. The consumption of some of these substances is prompted by religious beliefs and cultural reasons, making the legislation very variable or even ambiguous. However, the abusive consumption of these substances can present an enormous risk to the health of the individuals, since their metabolism and effects are not yet fully known. Additionally, NPSs are widely spread over the internet, and their appearance is very fast, which requires the development of sophisticated analytical methodologies, capable of detecting these compounds. Thus, the objective of this work is to review the toxicological aspects, traditional use/therapeutic potential and the analytical methods developed in biological matrices in twelve plant specimens (Areca catechu, Argyreia nervosa, Ayahuasca, Catha edulis, Datura stramonium, Lophophora williamsii, Mandragora officinarum, Mitragyna speciosa, Piper methysticum Forst, Psilocybe, Salvia divinorum and Tabernanthe iboga).

show abstract

Section: Mitragyna Speciosa (Kratom)mentioning

confidence: 99%

“…More recently, Basiliere et al [ 122 ] developed an analytical method using LC-Q/TOF-MS equipment for the quantification of mitragynine, 7-hydroxymitragynine, among other compounds. One milliliter of urine, pre-treated with SPE, was used, obtaining a LOD of 0.25–1 ng/mL and a LOQ of 0.5–1 ng/mL [ 122 ].…”

Section: Mitragyna Speciosa (Kratom)mentioning

confidence: 99%

Psychoactive Substances of Natural Origin: Toxicological Aspects, Therapeutic Properties and Analysis in Biological Samples

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Mitragynine, 7‐hydroxymitragynine, and mitrajavine are three of the psychoactive compounds found in kratom. Mitragynine and 7‐hydroxymitragynine have central nervous system activity and are agonists at kappa‐ and mu‐subtype opioid receptors 9 …”

Section: Overview Of Natural Productsmentioning

confidence: 99%

The analysis of commercially available natural products recommended for use in electronic cigarettes

Peace

Smith

Poklis

2020

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

Rationale: Natural plant products have been used to promote health, prevent sickness, and treat various ailments. These products often consist of leaves, flowers, bark, roots, seeds, and/or other parts of the plant. Many of the pharmacologically active constituents of these products are known, but the pharmacology of these constituents may not be fully elucidated. Natural plant-based products are also available in various forms other than the raw plant material. A wide array of commercial products such as capsules, powders, extracts, and electronic cigarette (e-cigarette) electronic liquids (e-liquids) are readily available and can be purchased from various outlets, both store-based retailers and online. Newer e-cigarettes are often advertised as "heat not burn" and are used for "vaping" various forms of extracts including "waxes" and "dabs" and raw plant material.Methods: A single manufacturer was found online selling "24 different herbs" in powders, extracts, or e-liquids. These were advertised as "legal in the USA" and each product listed multiple effects. Eight e-liquids, six extracts (resins), and four powders from eight different "herbs," namely African dream, areca nut, blue lotus, damiana, kra thum na, kra thum kok, klip dagga, and wild lettuce, were purchased. An advertisement for these products stated, "Most people use the leaves, powder or resin in vaporizers." Direct analysis in real time AccuTOF™ mass spectrometry (DART-MS) was used to identify the psychoactive components in the natural products. Results:The psychoactive compounds that were identified in only two of the eight e-liquids, three of the five resins, and three of the four powders were arecaidine, arecoline, coumarin, entadamide, mitragynine, 7-hydroxymitragynine, and nuciferine. Conclusions:Psychoactive and potentially harmful substances were present in the powders and resins of the natural products. The newer types of e-cigarettes made for consuming natural products may increase their abuse potential.

show abstract

“…7 The activity of kratom as an antimicrobial cannot be separated from the composition contained in the kratom leaves, including the high content of phenolics and flavonoids in the methanol extract of kratom leaves. 8 Phytochemical studies have shown that there are several chemical constituents of Mitragyna speciosa plants including indole alkaloids, including mitragynine, 7-hydroxymitraginine, 5-desmethylmitragynine, 17-desmethyldihydro-mitragynine, speciogynine, speciocilliatine, and paynantheine, 9 and also contain secondary metabolites such as flavonoids, saponin, monoterpenes, triterpenoids, secoirioids and polyphenolic compounds. 10 The antimicrobial potential of kratom is also expected to have an effect on the bacteria that cause respiratory and digestive tract infections.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial mechanism of Kratom (Mitragyna speciosa) methanol extract on Streptococcus pneumoniae and Eschericia coli bacteria

Salim¹,

Puspitarini

Setiwati³

et al. 2021

BHSJ

View full text Add to dashboard Cite

Introduction: Kratom (Mitragyna speciosa) is a tropical herbal plant native to the North Malay Peninsula, Malaysia and Thailand. In Indonesia, kratom us a typical plant of West Kalimantan, especially in Putussibau. Kratom leaf has many pharmacological effects, one of them is antioxidant. However, the antioxidant and antimicrobial activities of Mitragyna speciosa leaf extracts are lacking. This study was to examine the effect of the methanol extract of Mitragyna speciosa (Rubiaceae Family) leaves on microorganisms Escherichia coli and Strepococcus pneumoniae.Methods: Kratom leaves were extracted with methanol as solvent. In this study, S. pneumoniae and E. coli bacteria have been used. Antibacterial activity tests were carried out at concentrations of 0.78%, 1.56%, 3.125%, 6.25%, 12.5%, 25%, 50% and 100%. Insilico was used to search for secondary metabolites and metabolite interactions in Mytragina speciosa. Results: Mitragyna speciosa leaf methanol extract was effective in inhibiting the growth of E. coli and S. pneumoniae significantly (P<0.001). The growth of e.coli was not identified from a concentration of 25% to 100%. However, there was no bacterial growth in S. pneumoniae from a concentration of 6.25% to 100%. In the insilico study it was found that the secondary metabolite of Mitragyna speciosa is quercetin, which interacts with the ATP1 protein as a predictor in the mechanism of Mitragyna speciosa as an antibacterial. Conclusion: In conclusion, there is no correlation between nutritional status and remission outcome of patients with ALL in the induction phase of therapy. However, high percentage of underweight patients shows nutrition needs special attention to improve therapy outcomes.

show abstract

Identification of five Mitragyna alkaloids in urine using liquid chromatography-quadrupole/time of flight mass spectrometry

Cited by 29 publications

References 28 publications

Psychoactive Substances of Natural Origin: Toxicological Aspects, Therapeutic Properties and Analysis in Biological Samples

Psychoactive Substances of Natural Origin: Toxicological Aspects, Therapeutic Properties and Analysis in Biological Samples

The analysis of commercially available natural products recommended for use in electronic cigarettes

Antibacterial mechanism of Kratom (Mitragyna speciosa) methanol extract on Streptococcus pneumoniae and Eschericia coli bacteria

Contact Info

Product

Resources

About