Identification of foodborne pathogenic bacteria using confocal Raman microspectroscopy and chemometrics

Abstract

Rapid and accurate identification of foodborne pathogenic bacteria is of great importance because they are often responsible for the majority of serious foodborne illnesses. The confocal Raman microspectroscopy (CRM) is a fast and easy-to-use method known for its effectiveness in detecting and identifying microorganisms. This study demonstrates that CRM combined with chemometrics can serve as a rapid, reliable, and efficient method for the detection and identification of foodborne pathogenic bacteria without any laborious pre-treatments. Six important foodborne pathogenic bacteria including S. flexneri, L. monocytogenes, V. cholerae, S. aureus, S. typhimurium, and C. botulinum were investigated with CRM. These pathogenic bacteria can be differentiated based on several characteristic peaks and peak intensity ratio. Principal component analysis (PCA) was used for investigating the difference of various samples and reducing the dimensionality of the dataset. Performances of some classical classifiers were compared for bacterial detection and identification including decision tree (DT), artificial neural network (ANN), and Fisher’s discriminant analysis (FDA). Correct recognition ratio (CRR), area under the receiver operating characteristic curve (ROC), cumulative gains, and lift charts were used to evaluate the performance of models. The impact of different pretreatment methods on the models was explored, and pretreatment methods include Savitzky–Golay algorithm smoothing (SG), standard normal variate (SNV), multivariate scatter correction (MSC), and Savitzky–Golay algorithm 1st Derivative (SG 1st Der). In the DT, ANN, and FDA model, FDA is more robust for overfitting problem and offers the highest accuracy. Most pretreatment methods raised the performance of the models except SNV. The results revealed that CRM coupled with chemometrics offers a powerful tool for the discrimination of foodborne pathogenic bacteria.

Dissecting and tracing the gut microbiota of infants with botulism: a cross sectional and longitudinal study

“…Infant botulism is the major form of botulism in the United States (CDC, 2012) and has been found across the world (Drivenes et al, 2017;Dilena et al, 2021;Jeon et al, 2021;Avila et al, 2022;Douillard et al, 2022;Nga et al, 2022;Oliveira et al, 2022;Goldberg et al, 2023). Infant botulism was first reported in China in 1990 (Wu et al, 1990) and more cases have been reported in recent years with better diagnostic technologies (Xin et al, 2019;Ge et al, 2020;Zhang et al, 2022).…”

Establishment and comparison of in situ detection models for foodborne pathogen contamination on mutton based on SWIR-HSI

“…Optical rapid detection technology can use the unique optical characteristics of microbial samples to identify them, which opens up new avenues for microbial research. Due to their fast, green and easy operation, they have shown good application prospects in the detection of pathogenic bacteria in recent years (5)(6)(7). Among them, hyperspectral imaging (HSI) technology can simultaneously collect spatial, spectral and radiation information of the object to be measured, and has become one of the most powerful technologies for food quality and safety detection (8).…”

Dissecting and Tracing the Gut Microbiota of Infants with Botulism: A Cross Sectional and Longitudinal Study

“…Infant botulism accounted for around 70% of botulism cases over the past decade [5] and was found across the world [6][7][8][9][10][11][12][13]. Infant botulism was rstly reported in China in 1990 [14] and more cases have been reported in recent years with better diagnostic technologies [15][16][17].…”