Identification of Free Radical Scavengers from Brazilian Green Propolis Using Off‐Line HPLC‐DPPH Assay and LC‐MS

Zhang, Cuiping; Shen, Xiaoge; Chen, Jiawei; Jiang, Xuesheng; Hu, Fuliang

doi:10.1111/1750-3841.13730

Cited by 39 publications

(42 citation statements)

References 26 publications

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“…This activity is attributed mainly to the presence of flavonoids and polyphenols [ 28 ], among them, some caffeic and cinnamic acid derivatives [ 29 – 31 ]. EPP-AF® presents, unlike other propolis presentations studied, high content of artepillin C, another agent with marked antioxidant activity [ 32 ]. In this study, we showed the in vivo antioxidant effect of EPP-AF® on healthy young participants where low oxidation formation was expected and oxidative balance was preserved.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant Effect of Standardized Extract of Propolis (EPP‐AF®) in Healthy Volunteers: A “Before and After” Clinical Study

Diniz

Lorencini

Berretta

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Background. Propolis is rich in polyphenols, especially flavonoids and phenolic acids, and has significant antioxidant activity, shown mainly in “in vitro” studies. Objective. The aim of this study was to evaluate the antioxidant efficacy and safety of a standardized propolis extract in healthy volunteers. Design. A two-phase sequential, open-label, nonrandomized, before and after clinical trial. Methods. Healthy participants received two EPP-AF® doses (375 and 750 mg/d, P.O, tid) during 7 ± 2 days, starting with the lower doses. Immediately before starting EPP-AF® administration and at the end of each 7-day dosing schedule, blood and urine samples were collected for quantification of 8-OHDG (8-hydroxydeoxyguanosine) and 8-ISO (8-isoprostanes) in urine and GSH (reduced glutathione), GSSG (oxidized glutathione), SOD (superoxide dismutase), FRAP (Ferric Reducing Antioxidant Power), vitamin E, and MDA (malondialdehyde) in plasma. Results. In our study, we had 34 healthy participants (67.7% women, 30 ± 8 years old, 97% white). The 8-ISO, a biomarker of lipid peroxidation, decreased with both doses of EPP-AF® compared to baseline (8-ISO, 1.1 (0.9–1.3) versus 0.85 (0.75–0.95) and 0.89 (0.74–1.0), ng/mg creatinine, P < 0.05 , for 375 and 750 mg/d EPP-AF® doses versus baseline, mean and CI 95%, respectively). 8-OHDG, a biomarker of DNA oxidation, was also reduced compared to baseline with 750 mg/d doses (8-OHDG, 15.7 (13.2–18.1) versus 11.6 (10.2–13.0), baseline versus 750 mg/d, respectively, ng/mg creatinine, P < 0.05 ). Reduction of biomarkers of oxidative stress damage was accompanied by increased plasma SOD activity (68.8 (66.1–73.3) versus 78.2 (72.2–80.5) and 77.7 (74.1–82.6), %inhibition, P < 0.0001 , 375 and 750 mg/d versus baseline, median and interquartile range 25–75%, respectively) and by increased GSH for 375 mg/d EPP-AF® doses (1.23 (1.06–1.34) versus 1.33 (1.06–1.47), μmol/L, P < 0.05 ). Conclusion. EPP-AF® reduced biomarkers of oxidative stress cell damage in healthy humans, with increased antioxidant enzymatic capacity, especially of SOD. This trial is registered with the Brazilian Registry of Clinical Trials (ReBEC, RBR-9zmfs9).

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Section: Discussionmentioning

confidence: 99%

Antioxidant Effect of Standardized Extract of Propolis (EPP‐AF®) in Healthy Volunteers: A “Before and After” Clinical Study

Diniz

Lorencini

Berretta

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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“…In the same in vitro studies, the antioxidant capacity of propolis extracts was found to be similar to that of the synthetic antioxidant butylated hydroxytoluene or ascorbic acid [ 4 , 20 ]. Importantly, the antioxidant capacity of propolis is dependent on its content, but the studies aiming at finding the distinct relationships between these two parameters are not consistent [ 3 , 35 , 36 ]. In general, according to the literature data, the total phenolic content of propolis extracts ranged from about 30 to 200 mg of gallic acid equivalents (GAE)/g of dry weight, and the flavonoid content ranged from about 30 to 70 mg of quercetin equivalents (QE)/g, whereas DPPH free radical-scavenging activity ranged from about 20 to 190 μ g/mL [ 3 , 19 , 20 , 31 , 35 , 36 , 38 ].…”

Section: Propolis: “Bee Glue”mentioning

confidence: 99%

“…The phenolic compounds, but the different ones than the flavonoids, are believed to be responsible for the antioxidant activity of Brazilian propolis. According to Zhang et al [ 36 ], 3,4,5-tricaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, and artepillin C seem to be responsible for the strong antioxidant activity of Brazilian green propolis. Unlike in the case of Brazilian propolis, the antioxidant activity of poplar propolis appears to be largely influenced by both total polyphenol and total flavonoid contents [ 3 , 34 , 38 ].…”

Section: Propolis: “Bee Glue”mentioning

confidence: 99%

Antioxidant Potential of Propolis, Bee Pollen, and Royal Jelly: Possible Medical Application

Kocot

Kiełczykowska

Luchowska-Kocot

et al. 2018

Oxidative Medicine and Cellular Longevity

365

358

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Honeybees products comprise of numerous substances, including propolis, bee pollen, and royal jelly, which have long been known for their medicinal and health-promoting properties. Their wide biological effects have been known and used since antiquity. Bee products are considered to be a potential source of natural antioxidants such as flavonoids, phenolic acids, or terpenoids. Nowadays, the still growing concern in natural substances capable of counteracting the effects of oxidative stress underlying the pathogenesis of numerous diseases, such as neurodegenerative disorders, cancer, diabetes, and atherosclerosis, as well as negative effects of different harmful factors and drugs, is being observed. Having regarded the importance of acquiring drugs from natural sources, this review is aimed at updating the current state of knowledge of antioxidant capacity of selected bee products, namely, propolis, bee pollen, and royal jelly, and of their potential antioxidant-related therapeutic applications. Moreover, the particular attention has been attributed to the understanding of the mechanisms underlying antioxidant properties of bee products. The influence of bee species, plant origin, geographic location, and seasonality as well as type of extraction solutions on the composition of bee products extracts were also discussed.

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“…Then, 20 µL of the mixture was injected into the HPLC and analyzed as described above. A blank control of carob extracts with the same volume of methanol was also analyzed [23].…”

Section: Hplc Coupled On a Pre-column Dpph Assaymentioning

confidence: 99%

Utilization of Carob Fruit as Sources of Phenolic Compounds with Antioxidant Potential: Extraction Optimization and Application in Food Models

Goulas

Georgiou

2019

Foods

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The goal of this study was to explore the potential of carob extracts to act as lipid inhibitors in model food systems. First, the extraction efficacy of fourteen solvents on the phenolic and flavonoid contents as well as on the antioxidant activity was assessed. Results showed that the phenolic composition and antioxidant activity of the extracts were strongly affected by solvents. Subsequently, the antioxidant potential of the most promising extracts (water, methanol, acidic acetone, and acetone–water) against four model food systems were evaluated. The acidic acetone extract had the highest antioxidant activity (70.3 ± 5.3%) in the β-carotene-linoleic acid system, followed by the acetone–water extract (62.1 ± 4.9%). Both extracts significantly prevented the lipid oxidation in sunflower oil and cooked comminuted pork; the inhibition activity at the end of storage period was 36.7–50.5% and 17.4–24.8%, respectively. A reduction of 49.5–54.8% in the formation of dienes in the oil-in-water emulsion was also found. The inhibitory effect of methanolic and aqueous extracts was significantly lower. Qualitative and quantitative variations in extracts are responsible for this antioxidant behavior in food systems. Gallic acid, myrecetin, rutin, and catechin are the main components of the extracts while myricetin and quercetin play an essential role in the antioxidant activity, according to the biochromatograms.

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Identification of Free Radical Scavengers from Brazilian Green Propolis Using Off‐Line HPLC‐DPPH Assay and LC‐MS

Cited by 39 publications

References 26 publications

Antioxidant Effect of Standardized Extract of Propolis (EPP‐AF®) in Healthy Volunteers: A “Before and After” Clinical Study

Antioxidant Effect of Standardized Extract of Propolis (EPP‐AF®) in Healthy Volunteers: A “Before and After” Clinical Study

Antioxidant Potential of Propolis, Bee Pollen, and Royal Jelly: Possible Medical Application

Utilization of Carob Fruit as Sources of Phenolic Compounds with Antioxidant Potential: Extraction Optimization and Application in Food Models

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