Identification of frozen/thawed beef based on label-free detection of hemin (Iron Porphyrin) with solution-gated graphene transistor sensors

Gao, Shengjie; Wang, Rongrong; Bi, Yulong; Qu, Hao; Chen, Ying; Zheng, Lei

doi:10.1016/j.snb.2019.127167

Cited by 21 publications

(12 citation statements)

References 47 publications

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“…A large number of publications estimate the sensor specificity by monitoring real-time but absolute signal change induced by different substances . Although these absolute changes (e.g., effective gate voltage and Dirac shift) are efficient to indicate sensor specificity, it is difficult to compare differential sensors quantitatively for the device-to-device variations and buffer categories. , Therefore, the normalized sensor response and the ratio of target/nontarget response could be much more convincing assisted by statistical analysis. , In particular, the specificity and detection range should exceed the relevant range of targeted analytes.…”

Section: Fundamentals and Motivationmentioning

confidence: 99%

“…Proteins, composed of one or more amino acid chains, are present in all living organisms and perform many biological functions. , Thus, protein sensors have attracted tremendous attention in enzymatic activity, protein-to-protein interaction, disease diagnosis, and epidemic control . This section summarizes advances in 2D FET protein sensors by categorizing specific receptor probes (Table ).…”

Section: Biological Sensorsmentioning

confidence: 99%

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Two-Dimensional Field-Effect Transistor Sensors: The Road toward Commercialization

2022

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The evolutionary success in information technology has been sustained by the rapid growth of sensor technology. Recently, advances in sensor technology have promoted the ambitious requirement to build intelligent systems that can be controlled by external stimuli along with independent operation, adaptivity, and low energy expenditure. Among various sensing techniques, field-effect transistors (FETs) with channels made of two-dimensional (2D) materials attract increasing attention for advantages such as label-free detection, fast response, easy operation, and capability of integration. With atomic thickness, 2D materials restrict the carrier flow within the material surface and expose it directly to the external environment, leading to efficient signal acquisition and conversion. This review summarizes the latest advances of 2D-materials-based FET (2D FET) sensors in a comprehensive manner that contains the material, operating principles, fabrication technologies, proof-of-concept applications, and prototypes. First, a brief description of the background and fundamentals is provided. The subsequent contents summarize physical, chemical, and biological 2D FET sensors and their applications. Then, we highlight the challenges of their commercialization and discuss corresponding solution techniques. The following section presents a systematic survey of recent progress in developing commercial prototypes. Lastly, we summarize the long-standing efforts and prospective future development of 2D FET-based sensing systems toward commercialization.

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Section: Fundamentals and Motivationmentioning

confidence: 99%

Section: Biological Sensorsmentioning

confidence: 99%

Two-Dimensional Field-Effect Transistor Sensors: The Road toward Commercialization

2022

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“…However, due to its high protein content and nutritional value, beef is easily perishable and has a short shelf life. In this sense, freezing has been commonly adopted as the safest and most cost-effective method of meat preservation, used in both the meat industry and in household kitchens, and it also plays a vital role in both export–import and regional trade [ 4 , 5 ]. Furthermore, freezing effectively inhibits microbial, endogenous enzymes, oxygen, and heat-induced biochemical activity, making it ideal for extending meat shelf life [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

The Investigation of Protein Profile and Meat Quality in Bovine Longissimus thoracic Frozen under Different Temperatures by Data-Independent Acquisition (DIA) Strategy

Qian

Huang

et al. 2022

Foods

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The influence of freezing on the protein profile and quality traits in bovine Longissimus thoracic (LT) muscle was investigated by the data-independent acquisition (DIA) technique. Compared to fresh meat, a total of 262 proteins were identified as differential abundance proteins (DAPs) in four frozen groups (−12 °C, −18 °C, −38 °C, and −80 °C). According to the bioinformatics analysis, most of the DAPs in the significant Go terms and the KEGG pathway were structure proteins and enzymes. Proteome changes in the frozen bovine muscle at −12 °C and −18 °C were more significant than those at −38 °C and −80 °C. The result was consistent with the deterioration trend of the meat quality. The correlation analysis revealed that 17 proteins were correlated closely with the color, shear force, thawing loss, and cooking loss of the frozen meat, which could be used as putative biomarkers for frozen meat quality. MYO18A and ME3 are newly discovered proteins that are associated with frozen beef quality. In addition, CTTN and SERPINB6 were identified in frozen groups, which exhibited a significant inverse correlation with thawing loss (p < 0.01). These findings reveal the quality changes induced by freezing at the protein molecular level and provide new insights into the control of quality deterioration.

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“…with extremely high sensitivity due to the inherent signal amplification. Our previous studies have shown that SGGTs have also great potential for practical applications in detection of hazardous components in food samples for food safety inspection purposes. − However, when used as ion sensors, one of the greatest limitations for SGGTs is lack of selective response to a specific ion, since the detection mechanism is solely the change in dirac point voltages ( V dirac ) of the devices induced by nonspecific electrochemical reactions of ions at the gate , or changes in electrolyte conditions by these ions. , …”

Section: Introductionmentioning

confidence: 99%

DNA-Gated Graphene Field-Effect Transistors for Specific Detection of Arsenic(III) in Rice

Wang

et al. 2021

J. Agric. Food Chem.

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As one of the most toxic forms of arsenic, inorganic As(III) is easy to accumulate in rice, leading to severe public health problems. Effective control of As(III) requires the development of fast analytical methods for its detection with high sensitivity and specificity. Toward this end, in this work, we report the fabrication of an As(III) electrochemical sensor based on a solution-gated graphene transistor (SGGT) platform with a novel sensing mechanism. The gold gate electrode of the SGGT was modified with DNA probes and then blocked with bovine serum albumin (BSA). The specific interaction between As(III) and gold disrupted the adsorption states of DNA probes, redistributing surface charges on the gate electrode, further leading to potential drop changes at the interfaces of the gate electrode and graphene active layer. This new mechanism based on DNA-charge-redistributioninduced SGGT current responses (denoted as "DNA-SGGT") was found to greatly improve the selectivity of the sensor: the response of DNA-SGGT to As(III) was effectively enhanced fourfold, while to other interfering cations, it was significantly reduced. The optimized sensor showed a detection limit as low as 5 nM with superior selectivity to As(III). The as-prepared DNA-SGGTbased sensor has also been successfully applied to the detection of As(III) in practical rice samples with a high recovery rate, showing great potential for heavy metal detection in many types of food samples.

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Identification of frozen/thawed beef based on label-free detection of hemin (Iron Porphyrin) with solution-gated graphene transistor sensors

Cited by 21 publications

References 47 publications

Two-Dimensional Field-Effect Transistor Sensors: The Road toward Commercialization

Two-Dimensional Field-Effect Transistor Sensors: The Road toward Commercialization

The Investigation of Protein Profile and Meat Quality in Bovine Longissimus thoracic Frozen under Different Temperatures by Data-Independent Acquisition (DIA) Strategy

DNA-Gated Graphene Field-Effect Transistors for Specific Detection of Arsenic(III) in Rice

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