Identification of GABAB Receptor in Rat Testis and Sperm

He, Xiao; Hu, Jing; Wu, Qi; Yuan, Yan; Koide, S. S.

doi:10.1006/bbrc.2001.4732

Cited by 39 publications

(36 citation statements)

References 27 publications

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“…We have previously shown that GABA A and GABA B receptors are expressed in rat testis and sperm [14,15]. GABA B receptors are localized in the dorsal region of the acrosome of rat spermatozoa [15], while the GABA A receptors are located principally on the ventral region of the acrosome (unpublished data).…”

Section: Discussionmentioning

confidence: 79%

“…Activation of GABA B receptors has the long-lasting, modulatory e ects of in¯uencing presynaptic neurotransmission by decreasing Ca 2 ‡ conductanc e and by increasing the postsynaptic K ‡ conductance whereby generating late IPSPs [1,29]. GABA B receptors are present in rat testis and sperm [14], although its function has not been clari®ed. Since rat sperm contain both the GABA A and GABA B receptors, the present study was performed to determine the relationship of these subtypes of GABA receptors in regulating AR.…”

mentioning

confidence: 99%

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Biphasic Effect of GABA on Rat Sperm Acrosome Reaction: Involvement of GABA A and GABA B Receptors

et al. 2002

Archives of Andrology

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Section: Discussionmentioning

confidence: 79%

mentioning

confidence: 99%

Biphasic Effect of GABA on Rat Sperm Acrosome Reaction: Involvement of GABA A and GABA B Receptors

et al. 2002

Archives of Andrology

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“…Similar to progesterone, GABA was able to induce the acrosome reaction in human sperm at 0.5 mM [Castelli et al 1999]. Recent studies show that the GABA A and GABA B receptors were expressed in rat sperm, which might be part of the acrosome reaction mediated by GABA and progesterone [Shi and Roldan 1995;Calogero et al 1999;He et al 2001;. These findings suggested that GABA and its receptors were important components of the reproductive system.…”

Section: Introductionmentioning

confidence: 89%

Expression of GAT1 in Male Reproductive System and its Effects on Reproduction in Mice

Zhang

Gui

Yuan

et al. 2009

Systems Biology in Reproductive Medicine

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The present study was carried out to identify GABA (g-aminobutyric acid) transport protein I (GAT1) in male reproductive organs and to study the effect of GAT1 overexpression on the male reproductive system in GAT1 transgenic mice (TG). Expression and location of GAT1 in testes, epididymis, and sperm of wild-type (WT) mice were identified by immunohistochemistry and western-blot. Histological changes of testes, epididymis, and sperm of transgenic mice overexpressing GAT1 were detected by immunofluorenscent staining and haematoxylin and eosin (HE) staining. GAT1 expression was detected in the testes, epididymis, and sperm of non-transgenic mice. Vacuolization and deformity of spermatogenic cells were observed in the transgenic mice, but the epididymis was unremarkable. Immunofluorenscent staining showed that the number of diastrophic and decapitated sperm increased significantly in transgenic mice to 46.9% from 7.3% in nontransgenic mice. These results suggest that abnormal expression of GAT1 could result in spermiogenesis function injury, sperm paramorphia and dysgenesis.

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“…Moreover, GABA at relatively low concentrations (0.5 µM) mimics the effect of progesterone to induce the acrosome reaction in human spermatozoa [7]. Recent researches showed that GABA A and GABA B receptors were existed in rat testis and sperm [7][8][9], and these receptors might mediate the acrosome reaction induced by GABA and progesterone [10][11][12]. The findings suggested that GABA and its receptors played an important role in reproductive system.…”

Section: Introductionmentioning

confidence: 99%

Impaired reproduction in transgenic mice overexpressing γ-aminobutyric acid transporter I (GAT1)

Zhang

Hua

et al. 2004

Cell Res

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It is well documented that γ-aminobutyric acid (GABA) system existed in reproductive organs. Recent researches showed that GABA A and GABA B receptors were present in testis and sperm, and might mediate the acrosome reaction induced by GABA and progesterone. GABA transporter I (GAT1) also existed in testis and sperm, but its physiological function was unknown. In the present study, we used GAT1 overexpressing mice to explore GAT1 function in male reproductive system. We found that the expression level of GAT1 continuously increased in wild-type mouse testis from 1 month to 2 months after birth. GAT1 overexpression in mouse affected testis development, which embodied reduced testis mass and slowed spermatogenesis in transgenic mice. Moreover, transgenic mice showed increase of the percentage of broken sperm. The further study revealed that the reproductive capacity was impaired in GAT1 overexpressing mice. In addition, testosterone level was significantly low in transgenic mice compared with that in wild-type mice. Our findings provided the first evidence that abnormal expression of GAT1 could result in dysgenesis, and indicated that GAT1 might be therapeutically targeted for contraception or dysgenesis treatment.

show abstract

Identification of GABAB Receptor in Rat Testis and Sperm

Cited by 39 publications

References 27 publications

Biphasic Effect of GABA on Rat Sperm Acrosome Reaction: Involvement of GABA A and GABA B Receptors

Biphasic Effect of GABA on Rat Sperm Acrosome Reaction: Involvement of GABA A and GABA B Receptors

Expression of GAT1 in Male Reproductive System and its Effects on Reproduction in Mice

Impaired reproduction in transgenic mice overexpressing γ-aminobutyric acid transporter I (GAT1)

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