Identification of genes differentially expressed in association with acquired cisplatin resistance

Abstract

The goal of this study was to identify genes whose mRNA levels are differentially expressed in human cells with acquired cisplatin (cDDP) resistance. Using the parental UMSCC10b head and neck carcinoma cell line and the 5.9-fold cDDP-resistant subline, UMSCC10b/Pt-S15, two suppressive subtraction hybridization (SSH) cDNA libraries were prepared. One library represented mRNAs whose levels were increased in the cDDP resistant variant (the UP library), the other one represented mRNAs whose levels were decreased in the resistant cells (the DOWN library). Arrays constructed with inserts recovered from these libraries were hybridized with SSH products to identify truly differentially expressed elements. A total of 51 cDNA fragments present in the UP library and 16 in the DOWN library met the criteria established for differential expression. The sequences of 87% of these cDNA fragments were identified in Genbank. Among the mRNAs in the UP library that were frequently isolated and that showed high levels of differential expression were cytochrome oxidase I, ribosomal protein 28S, elongation factor 1α, α-enolase, stathmin, and HSP70. The approach taken in this study permitted identification of many genes never before linked to the cDDP-resistant phenotype. © 2000 Cancer Research Campaign

Role of cystine transport in intracellular glutathione level and cisplatin resistance in human ovarian cancer cell lines

“…The enhanced system x c À activity may result from the genetic changes that occurred in CDDP-resistant cells. Recently several genes, which are differentially expressed in association with CDDP resistance, have been identified (Johnsson et al, 2000). Among them the genes highly expressed in the resistant cells include cytochrome oxidase I, ribosomal protein 28S, elongation factor 1a, a-enolase, stathmin and HSP70.…”

EEF1A1 (eukaryotic translation elongation factor 1 alpha 1)

“…Note eEF1A1 overexpression is observed in cisplatinresistant human head and neck cancer cell lines (Johnsson et al, 2000).…”

Increased Expression of Dihydrodiol Dehydrogenase Induces Resistance to Cisplatin in Human Ovarian Carcinoma Cells

“…Indeed, utility of techniques such as suppressive subtraction hybridization in a cisplatinresistant head and neck cell line (16) and genome wide screening in yeast (17) have resulted recently in the identification of changes in the expression of such unlikely drug resistance candidate genes as cytochrome oxidase I, ribosomal protein 28 S, elongation factor 1␣, ␣-enolase, hsp70, PDE2, and ZDS2. However, the differential expression of these genes was not confirmed by quantitative PCR and/or Northern blot in either study.…”