Identification of Genes Involved in Flavonoid Biosynthesis of Chinese Narcissus (Narcissus tazetta L. var. chinensis)

Wang, Guiqing; Yang, Biyun; Wu, Jiacheng; Luo, Peng; Anwar, Muhammad; Allan, Andrew C.; Lin‐Wang, Kui; Espley, Richard V.; Zeng, Lihui

doi:10.1007/s11105-018-1119-6

Cited by 14 publications

(15 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The biosynthetic genes were strongly expressed during inflorescence development demonstrating the crucial importance of this step in the production of secondary metabolites. Consistently with previous transcriptomic studies in Lilium and in Narcissus for phenylpropanoids and flavonoids [64,65] and in Tanacetum cinerariifolium for sesquiterpene lactones [66], this approach allowed to functionally annotate these genes in C. cardunculus and provides valuable information for further investigations on their role as structural genes in the related pathways. Moreover, the use of RNA hybrid-seq enabled us to identify many alternatively spliced isoforms differentially expressed across the inflorescence development stages showing the complex transcriptional regulation system governing this process.…”

Section: Transcriptional Dynamics Of Identified C Cardunculus Secondsupporting

confidence: 78%

Hybrid transcriptome sequencing approach improved assembly and gene annotation in Cynara cardunculus (L.)

et al. 2020

View full text Add to dashboard Cite

Background: The investigation of transcriptome profiles using short reads in non-model organisms, which lack of well-annotated genomes, is limited by partial gene reconstruction and isoform detection. In contrast, long-reads sequencing techniques revealed their potential to generate complete transcript assemblies even when a reference genome is lacking. Cynara cardunculus var. altilis (DC) (cultivated cardoon) is a perennial hardy crop adapted to dry environments with many industrial and nutraceutical applications due to the richness of secondary metabolites mostly produced in flower heads. The investigation of this species benefited from the recent release of a draft genome, but the transcriptome profile during the capitula formation still remains unexplored. In the present study we show a transcriptome analysis of vegetative and inflorescence organs of cultivated cardoon through a novel hybrid RNA-seq assembly approach utilizing both long and short RNA-seq reads. Results: The inclusion of a single Nanopore flow-cell output in a hybrid sequencing approach determined an increase of 15% complete assembled genes and 18% transcript isoforms respect to short reads alone. Among 25, 463 assembled unigenes, we identified 578 new genes and updated 13,039 gene models, 11,169 of which were alternatively spliced isoforms. During capitulum development, 3424 genes were differentially expressed and approximately two-thirds were identified as transcription factors including bHLH, MYB, NAC, C2H2 and MADS-box which were highly expressed especially after capitulum opening. We also show the expression dynamics of key genes involved in the production of valuable secondary metabolites of which capitulum is rich such as phenylpropanoids, flavonoids and sesquiterpene lactones. Most of their biosynthetic genes were strongly transcribed in the flower heads with alternative isoforms exhibiting differentially expression levels across the tissues. Conclusions: This novel hybrid sequencing approach allowed to improve the transcriptome assembly, to update more than half of annotated genes and to identify many novel genes and different alternatively spliced isoforms. This study provides new insights on the flowering cycle in an Asteraceae plant, a valuable resource for plant biology and breeding in Cynara and an effective method for improving gene annotation.

show abstract

Section: Transcriptional Dynamics Of Identified C Cardunculus Secondsupporting

confidence: 78%

Hybrid transcriptome sequencing approach improved assembly and gene annotation in Cynara cardunculus (L.)

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Previous evidence has shown that perianths and coronas of Chinese narcissus have a higher level of flavonols, PAs are accumulated in basal plates, and no anthocyanidins are detected in all organs [21]. Expression analysis indicated that NtMYB3 has a higher expression level in basal plates than petals and corona, which contrasts with NtMYB2 , which has higher expression levels in petals and corona [19].…”

Section: Discussionmentioning

confidence: 99%

“…Expression analysis indicated that NtMYB3 has a higher expression level in basal plates than petals and corona, which contrasts with NtMYB2 , which has higher expression levels in petals and corona [19]. Previous studies have suggested that decreased expression of NtFLS and a lack of ANS expression lead to PAs accumulation in basal plates of Chinese narcissus [21]. Therefore, it is suggested that NtMYB3 may play a repressive role in basal plates of Chinese narcissus by down-regulating NtFLS expression.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies showed that PAs are accumulated in the scales, roots, and basal plates of Chinese narcissus, but not in flowers. A high expression level of the genes that encode the FLS or DFR steps play a key role in regulating flux through the different branches of the flavonoid biosynthetic pathway [21]. Understanding the regulation of different branches in flavonoid biosynthesis will provide knowledge to produce anthocyanins by genetic engineering in narcissus.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

NtMYB3, an R2R3-MYB from Narcissus, Regulates Flavonoid Biosynthesis

Anwar

Hong

et al. 2019

IJMS

Self Cite

View full text Add to dashboard Cite

R2R3-MYB transcription factors play important roles in the regulation of plant flavonoid metabolites. In the current study, NtMYB3, a novel R2R3-MYB transcriptional factor isolated from Chinese narcissus (Narcissus tazetta L. var. chinensis), was functionally characterized. Phylogenetic analysis indicated that NtMYB3 belongs to the AtMYB4-like clade, which includes repressor MYBs involved in the regulation of flavonoid biosynthesis. Transient assays showed that NtMYB3 significantly reduced red pigmentation induced by the potato anthocyanin activator StMYB-AN1 in agro-infiltrated leaves of tobacco. Over-expression of NtMYB3 decreased the red color of transgenic tobacco flowers, with qRT-PCR analysis showing that NtMYB3 repressed the expression levels of genes involved in anthocyanin and flavonol biosynthesis. However, the proanthocyanin content in flowers of transgenic tobacco increased as compared to wild type. NtMYB3 showed expression in all examined narcissus tissues; the expression level in basal plates of the bulb was highest. A 968 bp promoter fragment of narcissus FLS (NtFLS) was cloned, and transient expression and dual luciferase assays showed NtMYB3 repressed the promoter activity. These results reveal that NtMYB3 is involved in the regulation of flavonoid biosynthesis in narcissus by repressing the biosynthesis of flavonols, and this leads to proanthocyanin accumulation in the basal plate of narcissus.

show abstract

“…It is one of the top ten traditional flowers in China and has great application value in ornamental as well as refining essential oil (Jian et al 2006). The research on its flowering mechanism is also a hotspot topic in recent years (Wang et al 2018). Narcissus tazetta var.…”

mentioning

confidence: 99%

Complete plastome sequence of the Narcissus tazetta Var. chinensis: an important flower resource

Zhang

et al. 2020

Mitochondrial DNA Part B

View full text Add to dashboard Cite

Narcissus tazetta var. chinensis is an important economic perennial herb in China. In this study, the complete plastid genome was assembled from the next-generation sequencing data. The circular genome is 159,376 bp long, and comprises a pair of inverted repeat regions (28,492 bp for each), a large single-copy region (85,941 bp), and a small single-copy region (16,451 bp). It encodes 133 genes, including 87 protein-coding genes, 38 tRNAs genes, and 8 rRNA genes. The GC content of N. tazetta var. chinensis cp genome is 38%. Phylogenetic analysis indicates that N. tazetta var. chinensis was closely relate to Narcissus porticus. The complete plastome genome will provide a useful genetic resource for the conservation and garden utilization in the future.

show abstract

Identification of Genes Involved in Flavonoid Biosynthesis of Chinese Narcissus (Narcissus tazetta L. var. chinensis)

Cited by 14 publications

References 26 publications

Hybrid transcriptome sequencing approach improved assembly and gene annotation in Cynara cardunculus (L.)

Hybrid transcriptome sequencing approach improved assembly and gene annotation in Cynara cardunculus (L.)

NtMYB3, an R2R3-MYB from Narcissus, Regulates Flavonoid Biosynthesis

Complete plastome sequence of the Narcissus tazetta Var. chinensis: an important flower resource

Contact Info

Product

Resources

About