Identification of genes involved in flowering in Stevia rebaudiana using expressed sequence tags (ESTs)

Razali, Amin; Samsulrizal, Nurul Hidayah; Zainuddin, Zarina

doi:10.35118/apjmbb.2020.028.2.09

Cited by 2 publications

(1 citation statement)

References 30 publications

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“…Stevia rebaudiana Bertoni, also known as Stevia, is a perennial herbaceous plant belonging to the Asteraceae family [1]. S. rebaudiana can be utilised as a food and medicine due to its significant level of non-caloric sweetening flavouring ingredient [2][3][4][5][6]. The two major steviol glycosides, stevioside and rebaudioside A, are responsible for the sweet flavor of stevia [2].…”

Section: Introductionmentioning

confidence: 99%

In Silico Analysis and 3D Structure Prediction of Putative UDP-Glycosyltransferase 76G1 Protein in Stevia rebaudiana MS007

Mokhtar¹,

Samsulrizal

Khan³

et al. 2022

J.Trop Life Science

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Stevia rebaudiana is a plant of the Asteraceae family that is used as a natural sweetener. Stevia has been shown to be safe for human consumption and has been utilised as a sweetener substitute for diabetic and obese people. In this study, the structure and gene content involved in the synthesis of putative UDP-glycosyltransferase 76G1 (UGT76G1) protein in S. rebaudiana MS007 were analysed using an in-silico method. Homologous search using BlastP revealed the highest percentage of identity, score, and E-value for UDP-glycosyltransferase 76G1-like of Helianthus annuus (ID: XP_021973845.1). The presence of IPR002213 UDP-glucuronosyl/UDP-glucosyltransferase entry, which is available at locations 89bp to 246 bp, was also verified by the protein family search using InterPro. MEGA-X software was used to construct a molecular phylogeny study, revealing that this protein belongs to the Asteraceae family. To predict the primary, secondary, and tertiary protein structures of the putative UGT76G1 protein, the ProtParam, ExPasy, PSIPRED, and Phyre2 programmes were implemented. The putative UGT76G1 protein’s tertiary structure prediction was given a score of 100.0% confidence by the single highest scoring template and a coverage of 98%, with the dimension of the model being (Å) of X: 52.453, Y: 61.270, and Z: 48.102. The UGT76G1 model fulfilled the quality standards and was approved for further analysis after validation performed by PROCHECK, VERIFY3D, and ERRAT. Thus, the findings of this work have contributed to a better knowledge of putative UDP-glycosyltransferase 76G1 features and target recognition processes, which will lead to better information on protein-protein interaction in S. rebaudiana MS007. Keywords: Phylogenetic, Stevia rebaudiana, UGT76G1, 3D Structure Prediction

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Section: Introductionmentioning

confidence: 99%

In Silico Analysis and 3D Structure Prediction of Putative UDP-Glycosyltransferase 76G1 Protein in Stevia rebaudiana MS007

Mokhtar¹,

Samsulrizal

Khan³

et al. 2022

J.Trop Life Science

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Stevia rebaudiana: Reporting data from the sweetener crop for researchers and smallholder farming assistants

Carneiro,

Zanco,

Gonçalves

et al. 2024

Acta Sci. Agron.

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In science, technology and assistance to smallholder farming or large crop acreages of Stevia rebaudiana is necessary to report data using information components to facilitate investors to understand the local field conditions to make assertive economic decisions for long periods. Stevia rebaudiana produces consistent natural sweeteners with safe performance as reported in a multitude of scientific publications. The leaf chemicals components are suitable to humans with healthy disorders affected by the consumption of sugarcane or many other industrial sweeteners. Otherwise, aspects related to the agriculture of sweeteners production still require additional discussion to improve qualitative and quantitative traits necessary for cultivating cost-effective crops. The current text highlighted some agriculture decisions necessary to make the crop more profitable to investors as researchers, agriculture influencers, farmer assistants and consultants of companies looking for alternatives within agro-ecological systems.

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Identification of genes involved in flowering in Stevia rebaudiana using expressed sequence tags (ESTs)

Cited by 2 publications

References 30 publications

In Silico Analysis and 3D Structure Prediction of Putative UDP-Glycosyltransferase 76G1 Protein in Stevia rebaudiana MS007

In Silico Analysis and 3D Structure Prediction of Putative UDP-Glycosyltransferase 76G1 Protein in Stevia rebaudiana MS007

Stevia rebaudiana: Reporting data from the sweetener crop for researchers and smallholder farming assistants

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