Identification of Genes Related to Arsenic Detoxification in Rice Roots Using Microarray Analysis

Nguyen, Quynh-Thi Thuy; Huang, Tsai-Lien; Huang, Hsiang‐Po

doi:10.7763/ijbbb.2014.v4.304

Cited by 6 publications

(6 citation statements)

References 35 publications

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“…With application of metal(loid)s in the present study, an activation of the antioxidant apparatus in pigeonpea as well as pea indicated that increased oxidative burst is not attributed to the regulation of only one enzyme, but rather to the complex upregulation of several ROSscavenging enzymes (SOD, CAT, POX). An increase in the activity of antioxidant enzymes can be attributed to the induced transcription of their genes, probably mediated by free radicals (Duquesnoy et al, 2010;Nguyen et al, 2014;Ovečka and Takáč, 2014). In the present study, antioxidative activities varied among different organs, which could be related to the amount of metal(loid)s that accumulated differentially in the analyzed organs, to a much higher extent in roots than in leaves.…”

Section: Discussionmentioning

confidence: 48%

“…An increase in the activity of antioxidant enzymes can be attributed to the induced transcription of their genes, probably mediated by free radicals (Duquesnoy et al, 2010;Nguyen et al, 2014;Ovečka and Takáč, 2014). In the present study, antioxidative activities varied among different organs, which could be related to the amount of metal(loid)s that accumulated differentially in the analyzed organs, to a much higher extent in roots than in leaves.…”

Section: Discussionmentioning

confidence: 69%

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Metal uptake, oxidative metabolism, and mycorrhization in pigeonpeaand pea under arsenic and cadmium stress

Garg¹,

Singla²,

Bhandari³

2015

Turk J Agric For

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Bolan et al., 2014). The Comprehensive Environmental Response, Compensation, and Liability Act permanently listed As as no. 1 and Cd as no. 7 out of 275 in its priority list of hazardous materials (ATSDR, 2007).Arsenic and Cd toxicity cause oxidative stress by changing the composition and fluidity of membrane lipids, displacing essential metals in plant pigments or enzymes and thereby inactivating photosynthesis and respiration, and obstructing uptake and transport of water and nutrients, further reducing protein synthesis and carbohydrate metabolism along with diminished growth and yield (

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Section: Discussionmentioning

confidence: 48%

Section: Discussionmentioning

confidence: 69%

Metal uptake, oxidative metabolism, and mycorrhization in pigeonpeaand pea under arsenic and cadmium stress

Garg¹,

Singla²,

Bhandari³

2015

Turk J Agric For

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“…The As treatment affected transcription-, signaling- and hormone-related proteins, all of which are involved in the direct and retrograde communication between the nucleus and the organelles; their modulation could well disturb the normal development of the fruit [ 10 , 53 ]. Proteins related to transport and cell metabolism, also essential for ripening, probably served as a means for the tissue to sequester As into non-essential cellular structures and to increase the concentration of antioxidants needed to maintain an appropriate cellular redox state [ 54 ]. Supplementation with Si had a marked influence over the abundance of proteins associated with transcription, signaling and hormone metabolism, as also noted in the interaction of tomato with its pathogen Ralstonia solanacearum [ 55 ].…”

Section: Discussionmentioning

confidence: 99%

Target proteins reprogrammed by As and As + Si treatments in Solanum lycopersicum L. fruit

et al. 2017

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BackgroundArsenic is an important contaminant of many arable soils worldwide, while silicon, one of the most abundant elements in the earth’s crust, interacts with As in the context of plant metabolism. As toxicity results largely from its stimulation of reactive oxygen species, and it is believed that Si can mitigate this process through reduction of the level of oxidative stress. Experiments targeting the proteomic impact of exposure to As and Si have to date largely focused on analyses of root, shoot and seed of a range of mainly non-solanaceous species, thus it remains unclear whether oxidative stress is the most important manifestation of As toxicity in Solanum lycopersicum fruit which during ripening go through drastic physiological and molecular readjustments. The role of Si also needs to be re-evaluated.ResultsA comparison was drawn between the proteomic responses to As and As + Si treatments of the fruit of two tomato cultivars (cvs. Aragon and Gladis) known to contrast for their ability to take up these elements and to translocate them into fruits. Treatments were applied at the beginning of the red ripening stage, and the fruit proteomes were captured after a 14 day period of exposure. For each cultivar, a set of differentially abundant fruit proteins (from non-treated and treated plants) were isolated by 2DGE and identified using mass spectrometry. In the fruit of cv. Aragon, the As treatment reprogrammed proteins largely involved in transcription regulation (growth- regulating factor 9-like), and cell structure (actin-51), while in the cv. Gladis, the majority of differentially expressed proteins were associated with protein ubiquitination and proteolysis (E3 ubiquitin protein, and hormones (1-aminocyclopropane 1-carboxylase).ConclusionsThe present experiments were intended to establish whether Si supplementation can be used to reverse the proteomic disturbance induced by the As treatment; this reprogram was only partial and more effective in the fruit of cv. Gladis than in that of cv. Aragon. Proteins responsible for the protection of the fruits’ quality in the face of As-induced stress were identified. Moreover, supplementation with Si seemed to limit to a degree the accumulation of As in the tomato fruit of cv. Aragon.Electronic supplementary materialThe online version of this article (10.1186/s12870-017-1168-2) contains supplementary material, which is available to authorized users.

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“…Results showed that transgenic expression of both OsGrxs in A. thaliana was sufficient to increase plant tolerance to As. Earlier studies reported that expression of OsGrx_C7 and OsGrx_C2.1 was induced during As stress in rice in addition to defense and stress responsive genes, transporters, heat-shock proteins, metallothioneins, sulfate-metabolizing proteins ( Norton et al, 2008 ; Chakrabarty et al, 2009 ; Gautam et al, 2012 ; Nguyen et al, 2014 ). In this study, similar results were observed through RT-PCR analysis, upon AsIII and AsV exposure ( Figure 1 ) supporting the possible involvement of OsGrxs in As metabolism and transport.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of Rice Glutaredoxin OsGrx_C7 and OsGrx_C2.1 Reduces Intracellular Arsenic Accumulation and Increases Tolerance in Arabidopsis thaliana

Verma

Pande

et al. 2016

Front. Plant Sci.

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Glutaredoxins (Grxs) are a family of small multifunctional proteins involved in various cellular functions, including redox regulation and protection under oxidative stress. Despite the high number of Grx genes in plant genomes (48 Grxs in rice), the biological functions and physiological roles of most of them remain unknown. Here, the functional characterization of the two arsenic-responsive rice Grx family proteins, OsGrx_C7 and OsGrx_C2.1 are reported. Over-expression of OsGrx_C7 and OsGrx_C2.1 in transgenic Arabidopsis thaliana conferred arsenic (As) tolerance as reflected by germination, root growth assay, and whole plant growth. Also, the transgenic expression of OsGrxs displayed significantly reduced As accumulation in A. thaliana seeds and shoot tissues compared to WT plants during both AsIII and AsV stress. Thus, OsGrx_C7 and OsGrx_C2.1 seem to be an important determinant of As-stress response in plants. OsGrx_C7 and OsGrx_C2.1 transgenic showed to maintain intracellular GSH pool and involved in lowering AsIII accumulation either by extrusion or reducing uptake by altering the transcript of A. thaliana AtNIPs. Overall, OsGrx_C7 and OsGrx_C2.1 may represent a Grx family protein involved in As stress response and may allow a better understanding of the As induced stress pathways and the design of strategies for the improvement of stress tolerance as well as decreased As content in crops.

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Identification of Genes Related to Arsenic Detoxification in Rice Roots Using Microarray Analysis

Cited by 6 publications

References 35 publications

Metal uptake, oxidative metabolism, and mycorrhization in pigeonpeaand pea under arsenic and cadmium stress

Metal uptake, oxidative metabolism, and mycorrhization in pigeonpeaand pea under arsenic and cadmium stress

Target proteins reprogrammed by As and As + Si treatments in Solanum lycopersicum L. fruit

Overexpression of Rice Glutaredoxin OsGrx_C7 and OsGrx_C2.1 Reduces Intracellular Arsenic Accumulation and Increases Tolerance in Arabidopsis thaliana

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