Identification of genetic polymorphisms in FABP3 and FABP4 and putative association with back fat thickness in Korean native cattle

Cho, Seoae; Park, Toe Sung; Yoon, D. H.; Cheong, Hyun Sub; Namgoong, Suhg; Park, Byung Lae; Lee, Hye Won; Han, Chang Soo; Kim, Eun Mi; Cheong, I. C.; Kim, Hee Bal; Shin, Hyoung Doo

doi:10.5483/bmbrep.2008.41.1.029

Cited by 68 publications

(56 citation statements)

References 14 publications

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“…Studies have shown that FABP4 plays a critical role in fatty acid absorption and metabolism and seems to be involved in the hydrolysis of lipids (Michal et al, 2006 of Michal et al (2006), who observed an association between this gene and marbling score and backfat deposition in a Wagyu x Limousin F 2 population. Our results also conflict with those of Cho et al (2008), who found an association between 2 SNPs in FABP4 including the one investigated in our study and fat deposition in steers from the progeny testing program of the National Livestock Research Institute of Korea. Our previous association analysis with backfat data obtained by ultrasound in this Nelore population (data not shown) suggested a significant association between this gene and BFT.…”

Section: Resultscontrasting

confidence: 56%

“…From previous reports of effects on production traits (Bishop et al, 1994;Weikard et al, 2005;Cho et al, 2008), 3 candidate genes were selected for analysis. To amplify the promoter region of the IGF1 gene, which contains the microsatellite repeat (CA) n , primers described by Bishop et al (1994) were used, and the forward primer was labeled with fluorescence.…”

Section: Dna Extraction and Genotypic Datamentioning

confidence: 99%

“…The polymorphisms of PPARGC1A and FABP4 were genotyped with PCR-restriction fragment length polymorphism. To genotype the single-nucleotide polymorphism (G/A) in exon 2 of the FABP4 gene (Cho et al, 2008), we designed primers -forward, 5ꞌ-AATACACACACACACACCTGCTC-3ꞌ, and reverse, 5ꞌ-GAATGGCTTTCCTCCTTCTACA-3ꞌ -and used the restriction enzyme NmuCI. PCR was performed in 20-µL reactions with 1X buffer reaction, 1.5 mM MgCl 2 , 0.2 mM of each deoxyribonucleotide triphosphate, 0.4 μM of each primer, 1 U Taq DNA polymerase, and 100 ng genomic DNA.…”

Section: Dna Extraction and Genotypic Datamentioning

confidence: 99%

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Candidate genes for production traits in Nelore beef cattle

Tizioto

Meirelles²,

Tulio³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. Meat quality is an important trait for the beef industry. Backfat thickness, ribeye area, and shear force are traits measured late in life, and the investigation of molecular markers associated with these traits can help breeding programs. In cattle, some polymorphisms have been related to production traits. Thus, the purpose of this study was to assess the presence of polymorphisms in the candidate genes insulinlike growth factor 1 (IGF1), fatty acid-binding protein 4 (FABP4), and peroxisome proliferative active receptor gamma coactivator 1 A (PPARGC1A) and associate them with production traits in reference families of Nelore cattle. We used 270 steers descendent from 20 sires that were chosen to represent variability in this breed. The investigation of marker effects on the traits was performed using a mixed model under the restricted maximum likelihood method. A significant allele substitution effect was found for IGF1 and yearling weight (P ≤ 0.017). 4139©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 11 (4): 4138-4144 (2012) Candidate genes in NeloreThe mean allele substitution effect was 6.9 kg, with the 229 allele associated with reduced yearling weight in this Nelore population.

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Section: Resultscontrasting

confidence: 56%

Section: Dna Extraction and Genotypic Datamentioning

confidence: 99%

Section: Dna Extraction and Genotypic Datamentioning

confidence: 99%

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Candidate genes for production traits in Nelore beef cattle

Tizioto

Meirelles²,

Tulio³

et al. 2012

Genet. Mol. Res.

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“…However, this polymorphism was significantly associated with marbling and subcutaneous fat depth in Wagyu × Limousin F2 population (Michal et al, 2006) and in Korean native cattle (Cho et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

ANXA9, SLC27A3, FABP3 and FABP4 single nucleotide polymorphisms in relation to milk production traitsin Jersey cows

Kulig¹,

Kowalewska-Łuczak²,

Kmiec³

et al. 2010

CZECH J ANIM SCI

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Milk components originating from blood plasma substrates are synthesized in epithelial cells of the mammary gland. Milk lipids are synthesized from fatty acids which bind to specific proteins – FABPs (fatty acid binding proteins). FABPs are a family of small cytoplasmic proteins; nine members of the family have been identified so far (FABP1–FABP9) (Chmurzyńska et al., 2006). Their main roles include fatty acid uptake, transport and metabolism. FABPs can modulate the fatty acid concentration in cells and therefore they affect different cellular processes, especially lipid metabolism. FABP3 and FABP4 are present in tissues with a high demand for fatty acids, such as heart muscle, skeletal muscles, lactating mammary gland, liver or adipose tissue (Roy et al., 2003). FABP3 gene was mapped to bovine chromosome 2 (Calvo et al., 2004), where QTLs affecting milk fat yield and content were described (Khatkar et al., 2004). FABP4 gene was mapped to BTA14 (Michal et al., 2006), which is very rich in QTLs for milk production traits (Khatkar et al., 2004). Fatty acid transport is assisted by the specific proteins called FATPs (fatty acid transport proteins). This protein group includes SLC27A3 (solute carrier family 27, member 3). It belongs to the family of proteins that facilitate long-chain fatty acid transport across the cytoplasmic membrane. Another protein with similar functions is ANXA9 (annexin A9), the member of Ca2+ and phospholipid-binding protein family (Calvo et al., 2006b). Genes encoding SLC27A3 and ANXA9 were mapped to chromosome 3, within the region where QTLs for milk fat content and the other milk traits have been mapped. Both of the above-mentioned genes are expressed in the mammary gland (Calvo et al., 2006b). The polymorphic sites (SNPs – single nucleotide polymorphisms) within the bovine FABP3, FABP4, SLC27A3 and ANXA9 genes were identified (Wu et al., 2005; Calvo et al., 2006b; Michal et al., 2006; Cho et al., 2008). Associations between SNPs in these genes and milk production traits in cattle have not been reported so far. However, polymorphism in the FABP4 gene has been significantly associated with carcass traits in cattle (Michal et al., 2006; Cho et al., 2008). Due to their expression sites, physiological properties and chromosomal localisation, the described genes might be considered as candidate genes for milk production traits. The aim of this study was to determine allele and genotype frequencies and to establish possible associations between the ANXA9, SLC27A3, FABP3 and FABP4 SNPs, and selected milk traits in Jersey cows.

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“…These results are supported by previous studies where two alleles were reported in the CAST gene using the RFLP technique (Palmer et al 1998;Shahroudi et al 2006). Additionally, four alleles have been identified in the intron 12 region in New Zealand crossbred sheep by SSCP (Byun et al 2009 (Li et al 2010) and cattle (Cho et al 2008). Some variations in these genes are associated with milk fat content in sheep (Calvo et al 2004;Scata et al 2009 Identification of SNPs associated with mutton traits may not only help in improvement of the production but also provide economic gains to the rearers.…”

Section: Discussionmentioning

confidence: 99%

Identification of novel single nucleotide polymorphisms in candidate genes for mutton quality in Indian sheep

Arora

Yadav²,

Yadav³

2014

amb

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The study reports the identification of novel SNPs in candidate genes for mutton quality across a panel of eleven phenotypically and geographically diverse Indian sheep breeds. The investigated candidate genes were CAPN4, CAST, FABP3 and DGAT1 which are known to be polymorphic. Nine novel SNPs could be identified across the studied gene loci which were in the heterozygous condition. One SNP in the exon 1 of DGAT1 was non-synonymous. The average expected heterozygosity determined in the eleven Indian sheep breeds at these loci was 0.328. The allele and genotype frequencies for all the identified SNPs were determined. The average haplotype and nucleotide diversity across all loci were 0.545 and 0.206 respectively. The information generated provides preliminary indication of the functional diversity present in Indian sheep. Future studies will be required to establish the effect of the reported SNPs in the Indian sheep populations.

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Identification of genetic polymorphisms in FABP3 and FABP4 and putative association with back fat thickness in Korean native cattle

Cited by 68 publications

References 14 publications

Candidate genes for production traits in Nelore beef cattle

Candidate genes for production traits in Nelore beef cattle

ANXA9, SLC27A3, FABP3 and FABP4 single nucleotide polymorphisms in relation to milk production traitsin Jersey cows

Identification of novel single nucleotide polymorphisms in candidate genes for mutton quality in Indian sheep

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