Identification of genomic binding sites and direct target genes for the transcription factor DDIT3/CHOP

Transcriptional landscape of mitochondrial electron transport chain inhibition in renal cells

“…The authors propose DDIT3 to be protective in mitochondrial stress by preventing overactivation of ATF4 . DDIT3 is a basic leucine zipper transcription factor (bZIP TF) of the dimer-forming CAAT/Enhancer Binding Protein (C/EBP) family, forming homodimers with other bZIP TFs including C/EBPβ , ATF3 , MAF and ATF4 (Neill and Masson 2023 ; Osman et al 2023 ). All these genes were induced in the present study by ETC inhibitors.…”

“…All these genes were induced in the present study by ETC inhibitors. ChiP sequencing experiments has shown that DDIT3 overexpression, acts predominantly as a transcription repressor, but can also upregulate certain genes (Osman et al 2023 ). Interestingly, GADD45A , highly upregulated in this study, was amongst the gene targets supressed by DDIT3 overexpression (non-stressed conditions) (Osman et al 2023 ).…”

“…ChiP sequencing experiments has shown that DDIT3 overexpression, acts predominantly as a transcription repressor, but can also upregulate certain genes (Osman et al 2023 ). Interestingly, GADD45A , highly upregulated in this study, was amongst the gene targets supressed by DDIT3 overexpression (non-stressed conditions) (Osman et al 2023 ). It is plausible that ATF4/DDIT3 heterodimers exhibits different transcriptional outcomes and indeed GADD45A , which promotes cell cycle inhibition, has also been previously characterised as an ATF4 upregulated gene (Wang et al 2015 ).…”