Identification of genomic islands in Chilean <i>Piscirickettsia salmonis</i> strains and analysis of gene expression involved in virulence

Lagos, F; Cartes, C; Vera, Tamara; Haussmann, Denise; Figueroa, Jaime

doi:10.1111/jfd.12604

Cited by 13 publications

(11 citation statements)

References 37 publications

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“…The process was performed in a total volume of 20 μl using M‐MLV Reverse Transcriptase (Promega) according to the manufacturer's instructions. A preparation consisting of 1 μl (25 μM) of specific oligo (Lagos et al., 2017), 1 µl (10 mM) dNTP mix, 1 μg of total RNA and nuclease‐free water was set. It was incubated for 10 min at 60°C and cooled on ice.…”

Section: Methodsmentioning

confidence: 99%

Analysis of genes encoding for proteolytic enzymes and cytotoxic proteins as virulence factors of Piscirickettsia salmonis in SHK‐1 cells

Figueroa

Villagrán

Cartes

et al. 2021

Journal of Fish Diseases

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Piscirickettsia salmonis is the causative agent of Piscirickettsiosis, a systemic disease generating high mortality rates in farmed salmon cultures of southern Chile. Proteolytic enzymes are important virulence factors since they play a key role in bacterial invasion and proliferation within the host. Bacteria growing in muscle tissues are known to secrete proteases, but no proteolytic enzymes have been described in P. salmonis to date. A battery of putative protease genes was found in the genomes and available strains of P. salmonis by bioinformatics analyses, and their identity was established through comparison with protease genes in databases. The transcript levels of five candidate genes were analysed by in vitro infection and qPCR. All strains were found to generate protease activity to varying degrees, and this was significantly increased when bacteria infected a salmon cell line. Gene expression of several types of proteases was also evidenced, with the highest levels corresponding to the type 1 secretion system (T1SS), which is also involved in the transport of haemolysin A, although transcripts with significant levels of peptidase M4 (thermolysin) and CLP protease were also found.

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Section: Methodsmentioning

confidence: 99%

Analysis of genes encoding for proteolytic enzymes and cytotoxic proteins as virulence factors of Piscirickettsia salmonis in SHK‐1 cells

Figueroa

Villagrán

Cartes

et al. 2021

Journal of Fish Diseases

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“…Only one report of genomic islands exists for P. salmonis (Lagos et al . ). To obtain this information, bioinformatics analysis was performed on partially assembled complete genomes.…”

Section: Piscirickettsia Salmonismentioning

confidence: 97%

“…The assessed new strains had, on average, between 9 and 15 additional islands than LF‐89 (Lagos et al . ).…”

Section: Piscirickettsia Salmonismentioning

confidence: 97%

“…Comparisons were conducted against the Chilean P. salmonis strains and the type strain LF-89 (ATCC VR-1361). Indeed, two almost-parallel genomic studies on possible genes involved in conferring antibiotic resistance exist Cartes et al 2017). The MIC was determined for the type strain LF-89, which showed values ranging from 0.08 to 0.25 lg/mL for oxytetracycline and florfenicol.…”

Section: P Salmonis: Antibioticsmentioning

confidence: 99%

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Addressing viral and bacterial threats to salmon farming in Chile: historical contexts and perspectives for management and control

Figueroa

Cárcamo

Yáñez

et al. 2019

Reviews in Aquaculture

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The rapid growth of the salmon farming industry in Chile has led to the appearance of various viral, bacterial, parasitic, and fungal pathogens affecting farmed fish. The Chilean salmon industry has suffered several health crises associated with high fish mortalities, such as caused by the infectious salmon anaemia virus (2007) and harmful algal blooms (2016). In addition to these events, marine farms are continuously affected by outbreaks of harmful pathogens, including the bacteria Piscirickettsia salmonis and, most recently, a reappearance of Renibacterium salmoninarum, and the infectious pancreatic necrosis virus. These outbreaks have led to stricter regulations, but the salmon farming industry continues to struggle despite reforms. In addition to the aforementioned pathogens, which are a continuous threat, other apparently under control pathogens have also reappeared in recent years. In this review, we analyse the current state of knowledge on four of the main pathogens affecting salmon farming in Chile. The infectious pancreatic necrosis virus is relevant as it affects freshwater‐stage fish, with survivors then acting as carriers. The infectious salmon anaemia virus currently appears to be under control; however, P. salmonis and R. salmoninarum continue to be the cause for high mortalities in the Chilean aquaculture industry.

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“…Recently, pathogenic genomic islands have been identified in P . salmonis [ 16 ]. However, the repertoire and the potential roles of non-coding RNAs (ncRNAs) in P .…”

Section: Introductionmentioning

confidence: 99%

Core non-coding RNAs of Piscirickettsia salmonis

Segovia¹,

Arias-Carrasco²,

Yáñez³

et al. 2018

PLoS ONE

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Piscirickettsia salmonis, a fastidious Gram-negative intracellular facultative bacterium, is the causative agent o Piscirickettsiosis. P. salmonis has broad host range with a nearly worldwide distribution, causing significant mortality. The molecular regulatory mechanisms of P. salmonis pathogenesis are relatively unknown, mainly due to its difficult in vitro culture and genomic differences between genogroups. Bacterial non-coding RNAs (ncRNAs) are important post-transcriptional regulators of bacterial physiology and virulence that are predominantly transcribed from intergenic regions (trans-acting) or antisense strand of open reading frames (cis-acting). The repertoire of ncRNAs present in the genome of P. salmonis and its possible role in bacterial physiology and pathogenesis are unknown. Here, we predicted and analyzed the core ncRNAs of P. salmonis base on structure and correlate this prediction to RNA sequencing data. We identified a total of 69 ncRNA classes related to tRNAs, rRNA, thermoregulators, antitoxins, ribozymes, riboswitches, miRNAs and antisense-RNAs. Among these ncRNAs, 29 classes of ncRNAs are shared between all P. salmonis genomes, constituting the core ncRNAs of P. salmonis. The ncRNA core of P. salmonis could serve to develop diagnostic tools and explore the role of ncRNA in fish pathogenesis.

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Identification of genomic islands in Chilean Piscirickettsia salmonis strains and analysis of gene expression involved in virulence

Cited by 13 publications

References 37 publications

Analysis of genes encoding for proteolytic enzymes and cytotoxic proteins as virulence factors of Piscirickettsia salmonis in SHK‐1 cells

Analysis of genes encoding for proteolytic enzymes and cytotoxic proteins as virulence factors of Piscirickettsia salmonis in SHK‐1 cells

Addressing viral and bacterial threats to salmon farming in Chile: historical contexts and perspectives for management and control

Core non-coding RNAs of Piscirickettsia salmonis

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