Identification of genomic regions associated with cereal cyst nematode (Heterodera avenae Woll.) resistance in spring and winter wheat

Abstract

Cereal cyst nematode (CCN) is a major threat to cereal crop production globally including wheat (Triticum aestivum L.). In the present study, single-locus and multi-locus models of Genome-Wide Association Study (GWAS) were used to find marker trait associations (MTAs) against CCN (Heterodera avenae) in wheat. In total, 180 wheat accessions (100 spring and 80 winter types) were screened against H. avenae in two independent years (2018/2019 “Environment 1” and 2019/2020 “Environment 2”) under controlled conditions. A set of 12,908 SNP markers were used to perform the GWAS. Altogether, 11 significant MTAs, with threshold value of −log10 (p-values) ≥ 3.0, were detected using 180 wheat accessions under combined environment (CE). A novel MTA (wsnp_Ex_c53387_56641291) was detected under all environments (E1, E2 and CE) and considered to be stable MTA. Among the identified 11 MTAs, eight were novel and three were co-localized with previously known genes/QTLs/MTAs. In total, 13 putative candidate genes showing differential expression in roots, and known to be involved in plant defense mechanisms were reported. These MTAs could help us to identify resistance alleles from new sources, which could be used to identify wheat varieties with enhanced CCN resistance.

Mapping of QTLs and meta-QTLs for Heterodera avenae Woll. resistance in common wheat (Triticum aestivum L.)

“…This suggests that the level of resistance in different RILs inferred in R1 and R2 do not differ, which supports the conclusion that the differences are due to microenvironment. Similar results were also reported in the previous studies on CCN resistance in wheat from our own laboratory [ 39 , 40 ]. The occurrence of g×r interaction has perhaps interfered with detection of some QTLs for CCN resistance in both the repeats, so that none of the eight QTLs detected in the current study was discovered in both the repeats (R1 and R2), although some QTLs were detected in the combined data (CD).…”

Biotechnological Approaches for Plant Protection and Growth Promotion

“…In Solanum tuberosum, six different PIs were also identified [42][43][44][45][46][47][48][49][50]. Plant-parasitic nematodes (PPNs) are a serious global problem to cereal production [51][52][53][54][55]. Different types of PIs, such as cowpea trypsin inhibitor (CpTI), cystatins and serine proteinase inhibitors [56], have been reported to act following attacks by PPNs.…”