Identification of Genomic Regions Associated with Fusarium Wilt Resistance in Cowpea

Abstract

Fusarium wilt (FW), caused by the soil-borne fungal pathogen Fusarium oxysporum f. sp. Tracheiphilum, is a serious threat to cowpea production worldwide. Understanding the genetic architecture of FW resistance is a prerequisite to combatting this disease and developing FW resistance varieties. In the current study, a genetic diversity panel of 99 cowpea accessions was collected, and they were infected by a single strain, FW-HZ. The disease index (DI) based on the two indicators of leaf damage (LFD) and vascular discoloration (VD) varied highly across the population: most accessions were susceptible, and only seven accessions showed resistant phenotypes by both indicators. Through a genome-wide association study (GWAS), 3 and 7 single nucleotide polymorphisms (SNPs) significantly associated with LFD and VD were detected, respectively, which were distributed on chromosomes 3, 4, 5, 6 and 9, accounting for 0.68–13.92% of phenotypic variation. Based on the cowpea reference genome, 30 putative genes were identified and proposed as the likely candidates, including leucine-rich repeat protein kinase family protein, protein kinase superfamily protein and zinc finger family protein. These results provide novel insights into the genetic architecture of FW resistance and a basis for molecular breeding of FW resistant cultivars in cowpea.

A Salt-Tolerant Strain of Trichoderma longibrachiatum HL167 Is Effective in Alleviating Salt Stress, Promoting Plant Growth, and Managing Fusarium Wilt Disease in Cowpea

“…Fusarium oxysporum is a common soil-borne fungal plant pathogen that infects a wide range of host plants, including cowpea [ 13 , 14 ]. F. oxysporum infects cowpea roots and destroys the vascular cells, inhibiting growth and productivity, with yield losses reaching 70% [ 15 , 16 , 17 ]. Chemical fungicides are most often used to control this disease.…”

Genome-wide association study revealed genetic loci for resistance to fusarium wilt in tomato germplasm

“…GWA for Fusarium wilt resistance has been reported in many crop plants. Dong et al (2022) also identified 3 and 7 SNPs significantly associated with LFD and VD in response to Fusarium wilt in cowpea, respectively, on chromosomes 3, 4, 5, 6, and 9. Thirty candidate genes were identified, including leucine-rich repeat protein kinase family proteins, protein kinase superfamily proteins, and zinc finger family proteins.…”

“…This powerful approach allows the detection of genes/quantitative trait loci (QTLs) controlling complex traits, and tightly linked markers can be developed for markerassisted selection (MAS). However, successful GWA for Fusarium wilt resistance has been reported in many crop plants, such as cowpea (Dong et al 2022), castor bean (Shaw et al 2022), sweet basil (Gonda et al 2022), strawberry (Pincot et al 2018), cape gooseberry (Osorio-Guarín et al 2016), cotton (Mei et al 2014), pigeon pea (Patil et al 2017), and bottle gourd (Li et al 2021), but not in tomatoes. Therefore, this study aims to identify SNP markers linked to Fusarium wilt resistance in the natural germplasm of tomatoes using the genome-wide association approach.…”

Parameter Optimization Design of Precision Seeding Device Based on the Bp Neural Network for Panax Notoginseng

“…The BP neural network-based constrained optimization method is a new approach for the optimization of black box problems and includes single-objective optimization methods and multiobjective optimization methods (Dong et al, 2021;Wang et al, 2017;Dong et al, 2018a;Dong et al, 2019). The proposed method is based on the principle of iterative optimization via mathematical programming methods and the functional relationship fitting of BP neural networks.…”