Identification of glabridin as a bioactive compound in licorice (Glycyrrhiza glabra L.) extract that activates human peroxisome proliferator-activated receptor gamma (PPARγ)

Rebhun, John F.; Glynn, Kelly M.; Missler, Stephen R.

doi:10.1016/j.fitote.2015.08.004

Cited by 30 publications

(11 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cell lines containing two different vectors were needed to evaluate activation of the individual LBD of ERα and/or ERβ. These stable cell lines were generated by first transfecting CHO-K1 cells with pGL4.35 [luc2P/9XGAL4 UAS/Hygro] as described previously 21, 23. The ERα and ERβ LBD containing cell lines were then created by transfecting the stable pGL4.35 9XUAS Luc2P CHO-K1 cell line with the pBIND Gal4-ERα-LBD or pBIND3 Gal4-ERβ-LBD and selecting pBIND Gal4-ER-LBD containing cells with 600 μg/mL of G418.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of selected traditional Chinese medical extracts for bone mineral density maintenance: A mechanistic study

Rebhun¹,

Du²,

Hood³

et al. 2019

Journal of Traditional and Complementary Medicine

Self Cite

View full text Add to dashboard Cite

Objective To investigate the development of a minimal traditional Chinese medicine (TCM) formula using selected TCM ingredients and evaluating their biological activity with bone-specific in vitro tests. Finally, determining if the minimal formula can maintain bone mineral density (BMD) in a low bone mass (LBM)/osteoporosis (OP) model system. Methods and results Sixteen different TCM plant extracts were tested for estrogenic, osteogenic and osteoclastic activities. Despite robust activation of the full-length estrogen receptors α and β by Psoralea corylifolia and Epimedium brevicornu , these extracts do not activate the isolated estrogen ligand binding domains (LBD) of either ERα or ERβ; estrogen (17-β estradiol) fully activates the LBD of ERα and ERβ. E. brevicornu and Drynaria fortunei extracts activated cyclic AMP response elements (CRE) individually and when combined these ingredients stimulated the production of osteoblastic markers Runx2 and Bmp4 in MC3T3-E1 cells. E. brevicornu , Salvia miltiorrhiza , and Astragalus onobrychis extracts inhibited the Il-1β mediated activation of NF-κβ and an E. brevicornu / D. fortunei combination inhibited the development of osteoclasts from precursor cells. Further, a minimal formula containing the E. brevicornu / D. fortunei combination with or without a third ingredient ( S. miltiorrhiza, Angelica sinensis, or Lycium barbarum ) maintained bone mineral density (BMD) similar to an estradiol-treated control group in the ovariectomized rat; a model LBM/OP system. Conclusion A minimal formula consisting of TCM plant extracts that activate CRE and inhibit of NF-κβ activation, but do not behave like estrogen, maintain BMD in a LBM/OP model system.

show abstract

Section: Methodsmentioning

confidence: 99%

Evaluation of selected traditional Chinese medical extracts for bone mineral density maintenance: A mechanistic study

Rebhun¹,

Du²,

Hood³

et al. 2019

Journal of Traditional and Complementary Medicine

Self Cite

View full text Add to dashboard Cite

show abstract

“…The two groups were both blank control groups. In addition, cells were cultured by adding different concentrations of T0070907 for 12 h (0, 20, 40 and 50 µmol/L) [ 26 , 27 , 28 , 29 , 30 , 31 ] and then adding 4 µmol/L of DBDCT for 24 h.…”

Section: Methodsmentioning

confidence: 99%

Proteomics Analysis Reveals an Important Role for the PPAR Signaling Pathway in DBDCT-Induced Hepatotoxicity Mechanisms

Liu

Lin

et al. 2017

Molecules

View full text Add to dashboard Cite

A patented organotin di-n-butyl-di-(4-chlorobenzohydroxamato)tin (DBDCT) with high a antitumor activity was designed, however, its antitumor and toxic mechanisms have not yet been clearly illustrated. Hepatic proteins of DBDCT-treated rats were identified and analyzed using LC-MS/MS with label-free quantitative technology. In total, 149 differentially expressed proteins were successfully identified. Five protein and mRNA expressions were involved in the peroxisome proliferator-activated receptor (PPAR) signaling pathway, including a scavenger receptor (CD36), adipocyte fatty acid binding protein 4 (FABP4), enoyl-CoA hydratase (EHHADH), acetyl-CoA acyltransferase 1 (ACAA1), and phosphoenolpyruvate carboxykinase (PEPCK) in DBDCT-treated Rat Liver (BRL) cells. PPAR-α and PPAR-λ were also significantly decreased at both protein and mRNA levels. Furthermore, compared with the DBDCT treatment group, a special blocking agent of PPAR-λ T0070907 was used to evaluate the relationship between PPAR-λ and its downstream genes. Our studies indicated that DBDCT may serve as a modulator of PPAR-λ, further up-regulating CD36, FABP4 and EHHADH on the PPAR signal pathway.

show abstract

“…Glycyrrhizin (GL) is a triterpene glycoside extracted from Glycyrrhiza uralensis Fisher (Fabaceae) . GL has many pharmacological effects such as anti‐inflammatory, anti‐viral, anti‐oxidation and immunomodulatory activities .…”

Section: Introductionmentioning

confidence: 99%

“…22,27 Glycyrrhizin (GL) is a triterpene glycoside extracted from Glycyrrhiza uralensis Fisher (Fabaceae). 28 GL has many pharmacological effects such as anti-inflammatory, anti-viral, antioxidation and immunomodulatory activities. 29,30 Studies have shown that GL could effectively inhibit the carbon tetrachlorideinduced liver damage, liver fibrosis and other liver diseases.…”

Section: Introductionmentioning

confidence: 99%

Injectable hydrogels based on glycyrrhizin, alginate, and calcium for three‐dimensional cell culture in liver tissue engineering

Xiao-fang

Zhao

Ren

et al. 2018

J Biomedical Materials Res

View full text Add to dashboard Cite

Injectable hydrogels have been paid more attentions on cell therapy and tissue regeneration resulting from the applications in minimally invasive surgical procedures with ease of handling and complete filling of defect area. Here, a biodegradable and injectable in situ hydrogel formed by glycyrrhizin (GL), alginate (Alg), and calcium (Ca) was developed for three‐dimensional (3D) cell culture. Differential scanning calorimetry (DSC), X‐ray diffraction (XRD), scanning electron microscope (SEM) and rheology analysis were performed to characterize GL‐Alg‐Ca hydrogel and evaluate its formation mechanism, properties, and morphology. The biocompatibility of hydrogel was investigated by cell viability, morphology, and liver specific functions. The results of DSC, XRD, and rheology suggested that hydrogel was homogenous complex with stable structure and well viscoelasticity. Human hepatoma HepG2 cells cultured in hydrogels showed well morphology. Compared with the control group, cells in hydrogels showed good biocompatibility, and could maintain the viability, proliferation and liver function for longer periods of time. Furthermore, the hydrogel improved the mRNA expression of cytochrome P450, which were key enzyme to the metabolization of hepatocytes. The GL‐Alg‐Ca hydrogel could be a potential 3D cells culture system for liver tissue engineering. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 3292–3302, 2018.

show abstract

Identification of glabridin as a bioactive compound in licorice (Glycyrrhiza glabra L.) extract that activates human peroxisome proliferator-activated receptor gamma (PPARγ)

Cited by 30 publications

References 35 publications

Evaluation of selected traditional Chinese medical extracts for bone mineral density maintenance: A mechanistic study

Evaluation of selected traditional Chinese medical extracts for bone mineral density maintenance: A mechanistic study

Proteomics Analysis Reveals an Important Role for the PPAR Signaling Pathway in DBDCT-Induced Hepatotoxicity Mechanisms

Injectable hydrogels based on glycyrrhizin, alginate, and calcium for three‐dimensional cell culture in liver tissue engineering

Contact Info

Product

Resources

About