Identification of GmGPATs and their effect on glycerolipid biosynthesis through seed-specific expression in soybean

Liu, Hongbo; Wei, Limin; Zhu, Jinbo; Zhang, Bingxin; Gan, Yi; Zheng, Yueping

doi:10.1007/s11033-022-07852-w

Cited by 9 publications

(5 citation statements)

References 31 publications

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Section: Resultsmentioning

confidence: 99%

“…Liu et al showed that only GmGPAT9-2 (Glyma.09G119200) out of sixteen tested GmGPATs with high acyltransferase activity may not play a direct role in TAG formation. However, they found that seed-specific expression of GmGPAT9-2 in Arabidopsis increased the proportion of arachidic acid (C20:0) and erucic acid (C22:1) without an increase in the total oil content (H. Liu et al 2022). Except for Glyma.08G085800, the GmGPATs mentioned above were corroborated by Liu et al However, we found that this gene is highly expressed in seeds, specially in embryo and cotyledons (Figure 2.B, Supplementary Table 7), supporting its role in TAG synthesis.…”

Section: Candidate Oil Families and Expression Patterns Of Genes From...mentioning

confidence: 99%

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Multiomic analysis of genes related to oil traits in legumes provide insights into lipid metabolism and oil richness in soybean

Turquetti-Moraes,

Cardoso-Silva,

Almeida-Silva

et al. 2024

Preprint

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Soybean (Glycine max) and common bean (Phaseolus vulgaris) diverged approximately 19 million years ago. While these species share a whole-genome duplication (WGD), the Glycine lineage experienced a second, independent WGD. Despite the significance of these WGDs, their impact on gene families related to oil-traits remains poorly understood. Here, we report an in-depth investigation of oil-related gene families in soybean, common bean, and twenty-eight other legume species. We adopted a systematic approach that included transcriptome and co-expression analysis, identification of orthologous groups, gene duplication modes and evolutionary rates, and family expansions and contractions. We curated a list of oil candidate genes and found that 91.5% of the families containing these genes expanded in soybean in comparison to common bean. Notably, we observed an expansion of triacylglycerol (TAG) biosynthesis (~3:1) and an erosion of TAG degradation (~1.4:1) families in soybean in comparison to common bean. In addition, TAG degradation genes were two-fold more expressed in common bean than in soybean, suggesting that oil degradation is also important for the sharply contrasting seed oil contents in these species. We found 17 transcription factor hub genes that are likely regulators of lipid metabolism. Finally, we inferred expanded and contracted families and correlated these patterns with oil content found in different legume species. In summary, our results do not only shed light on the evolution of oil metabolism genes in soybean, but also present multifactorial evidence supporting the prioritization of candidates for crop improvement.

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Section: Resultsmentioning

confidence: 99%

Section: Candidate Oil Families and Expression Patterns Of Genes From...mentioning

confidence: 99%

Multiomic analysis of genes related to oil traits in legumes provide insights into lipid metabolism and oil richness in soybean

Turquetti-Moraes,

Cardoso-Silva,

Almeida-Silva

et al. 2024

Preprint

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“…The network of uptake and transport of inorganics and organics were carried out through vascular tissue [ 82 ], and it usually existed in all vegetative tissues in shoots and roots. Compared with other tissues, studies about seed-specific expression in soybean have been widely reported [ 14 , 83 , 84 , 85 , 86 , 87 , 88 ].…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Tissue-Specific Genes Identification for Novel Tissue-Specific Promoters Discovery in Soybean

Zhang

Guan

et al. 2023

Genes

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Promoters play a crucial role in controlling the spatial and temporal expression of genes at transcriptional levels in the process of higher plant growth and development. The spatial, efficient, and correct regulation of exogenous genes expression, as desired, is the key point in plant genetic engineering research. Constitutive promoters widely used in plant genetic transformation are limited because, sometimes, they may cause potential negative effects. This issue can be solved, to a certain extent, by using tissue-specific promoters. Compared with constitutive promoters, a few tissue-specific promoters have been isolated and applied. In this study, based on the transcriptome data, a total of 288 tissue-specific genes were collected, expressed in seven tissues, including the leaves, stems, flowers, pods, seeds, roots, and nodules of soybean (Glycine max). KEGG pathway enrichment analysis was carried out, and 52 metabolites were annotated. A total of 12 tissue-specific genes were selected via the transcription expression level and validated through real-time quantitative PCR, of which 10 genes showed tissue-specific expression. The 3-kb 5′ upstream regions of ten genes were obtained as putative promoters. Further analysis showed that all the 10 promoters contained many tissue-specific cis-elements. These results demonstrate that high-throughput transcriptional data can be used as effective tools, providing a guide for high-throughput novel tissue-specific promoter discovery.

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“…In the yeast genetic complementation assay, the yeast mutant ZAFU1 ( BY4742 , gat1Δgat2Δ +[ pGAL1::AtGPAT1 LEU2 ]) was used for screening acyltransferase activity. Subsequently, yeast genetic complementation procedures were conducted according to Lei et al [ 20 ] and Liu et al [ 24 ]. In a serial dilution (1:5) assay, a single clone was inoculated into SC-Ura-His-Leu + galactose or glucose medium with an initial optical density (OD 600 ) of 1.0 and cultured for 5 d at 30 °C.…”

Section: Methodsmentioning

confidence: 99%

The functional divergence of homologous GPAT9 genes contributes to the erucic acid content of Brassica napus seeds

Liu,

Zhu,

Zhang

et al. 2024

BMC Plant Biol

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Background The early allopolyploid Brassica napus was a hybrid of two Brassica species, that had undergone a whole genome duplication event followed by genome restructuring, including deletions and small scale duplications. A large number of homologous genes appeared functional divergence during species domestication. Due to the high conservation of de novo glycerolipid biosynthesis, multiple homologues of glycerol-3-phosphate acyltransferases (GPATs) have been found in B. napus. Moreover, the functional variances among these homologous GPAT-encoding genes are unclear. Results In this study, four B. napus homologous genes encoding glycerol-3-phosphate acyltransferase 9 (BnaGPAT9) were characterized. Although a bioinformatics analysis indicated high protein sequence similarity, the homologues demonstrated tissue-specific expression patterns and functional divergence. Yeast genetic complementation assays revealed that BnaGPAT9-A1/C1 homologues but not BnaGPAT9-A10/C9 homologues encoded functional GPAT enzymes. Furthermore, a single nucleotide polymorphism of BnaGPAT9-C1 that occurred during the domestication process was associated with enzyme activity and contributed to the fatty acid composition. The seed-specific expression of BnGPAT9-C11124A increased the erucic acid content in the transformant seeds. Conclusions This study revealed that BnaGPAT9 gene homologues evolved into functionally divergent forms with important roles in erucic acid biosynthesis.

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Identification of GmGPATs and their effect on glycerolipid biosynthesis through seed-specific expression in soybean

Cited by 9 publications

References 31 publications

Multiomic analysis of genes related to oil traits in legumes provide insights into lipid metabolism and oil richness in soybean

Multiomic analysis of genes related to oil traits in legumes provide insights into lipid metabolism and oil richness in soybean

Genome-Wide Tissue-Specific Genes Identification for Novel Tissue-Specific Promoters Discovery in Soybean

The functional divergence of homologous GPAT9 genes contributes to the erucic acid content of Brassica napus seeds

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