No abstract

Cyclophilins, a type of peptidyl-prolyl cis-trans isomerase, function as important molecular chaperones in a series of biological processes. However, the expression pattern and signal transduction pathway of cyclophilins are still unclear. Here, we showed that the promoter of OsCYP2 could function as a tissue-specific promoter by GUS staining. Moreover, we found that the promoter sequence contained not only core elements but also inducible elements. Then, the ABA-responsive element was used for cDNA library screening, and the transcription factor MYC2-like was identified by a yeast one-hybrid assay and confirmed through an electrophoretic mobility shift assay. Furthermore, the relative expression showed that MYC2-like was induced by abscisic acid. In addition, MYC2-like overexpression enhanced salt tolerance in transformants and partially restored the cyp2-RNAi line. In summary, we explored a novel transcriptional signal mediated by MYC2-like, a potential regulator of salt stress-related physiological processes in rice.

Cyclophilins, a type of peptidyl-prolyl cis-trans isomerase, function as an important molecular chaperone in series of biological processes. However, the expression pattern and signal transduction pathway of cyclophilins is still unclear. Hereby, it was clarified that the promoter of OsCYP2 is a constitutive expression pattern which by GUS staining in transformants with pOsCYP2:GUS genetic transformation. Simultaneously, it is found that the sequence of promoter had not only core elements but also contained inducible elements. Then, the ABA responsive element was used to cDNA library screening, a transcription factor myc2-like was identified by yeast one hybrid and confirmed though electrophoretic mobility shift assay. Furthermore, the relative expression showed that myc2-like was induced by abscisic acid. In addition, myc2-like overexpression could enhance salt tolerance in transformants and partial restore cyp2-RNAi line. Together, we explored a novel transcriptional signal mediated by myc2-like, a potential regulator of salt stress physiological processes in rice.