Identification of heat shock protein 27 as a radioresistance-related protein in nasopharyngeal carcinoma cells

Zhang, Bin; Qu, Jia-Quan; Liang, Xiao; Yi, Hong; Zhang, Peng-Fei; Li, Maoyu; Hu, Rong; Wan, Xun-Xun; He, Qianyu; Li, Jianhuang; Xu, Ye; Xiao, Zhi‐Qiang; Feng, Xueping

doi:10.1007/s00432-012-1293-0

Cited by 25 publications

(23 citation statements)

References 33 publications

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“…Expression of HSPB1 also correlated with tumor differentiation in esophageal squamous cell carcinoma 27. HSPB1 has been reported as a radioresistance-related protein in the NPC cell lines, inhibition of which enhanced radiosensitivity 28. Accordingly, the reduced expression of HSPB1 in the poorly differentiated might associate with the clinical relevance of the NPC histological types that differentiation in fact conferred a worse response to radiotherapy in endemic area 8.…”

Section: Discussionmentioning

confidence: 89%

Proteomic Characterization Reveals a Molecular Portrait of Nasopharyngeal Carcinoma Differentiation

Xiao

et al. 2017

J. Cancer

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Nasopharyngeal carcinoma (NPC) is categorized into three different differentiated subtypes by World Health Organization (WHO). Based on an earlier comparative proteomic database of the three histological subtypes, the study was to deepen our understanding of molecular mechanisms associated with NPC differentiation through bio-information mining. Among the three subtypes were 194 differentially expressed proteins (DEPs) of 725 identified proteins. Two DEPs, heat shock protein family B (small) member 1 (HSPB1) and keratin 5 (KRT5), were validated in a series of NPC tissue samples by using immunohistochemistry. Quantified protein families including keratins, S100 proteins (S100s) and heat shock proteins exhibited characteristic expression alterations. Comparisons of predicted bio-function activation states among different subtypes, including formation of cellular protrusion, metastasis, cell death, and viral infections, were conducted. Canonical pathway analysis inferred that Rho GTPases related signaling pathways regulated the motility and invasion of dedifferentiated NPC. In conclusion, the study explored the proteomic characteristics of NPC differentiation, which could deepen our knowledge of NPC tumorigenesis and allow the development of novel targets of therapeutic and prognostic value in NPC.

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Section: Discussionmentioning

confidence: 89%

Proteomic Characterization Reveals a Molecular Portrait of Nasopharyngeal Carcinoma Differentiation

Xiao

et al. 2017

J. Cancer

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“…Radioresistant human NPC cell lines CNE2-IR and CNE1-IR cells as well as their corresponding radiosensitive cell lines CNE2 and CNE1 cells were previously established by us (36,37), and cultured with RPMI-1640 medium containing 10% FBS (Invitrogen). Radioresistant CNE2-IR and CNE1-IR cells were derived from parental CNE2 and CNE1 cells, respectively, by treating the cells with four rounds of sublethal ionizing radiation (36,37).…”

Section: Cell Linesmentioning

confidence: 99%

“…Radioresistant CNE2-IR and CNE1-IR cells were derived from parental CNE2 and CNE1 cells, respectively, by treating the cells with four rounds of sublethal ionizing radiation (36,37). Radiosensitive CNE2 and CNE1 cells, used as a control, were treated with the same procedure except sham irradiated.…”

Section: Cell Linesmentioning

confidence: 99%

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MiRNA-203 Reduces Nasopharyngeal Carcinoma Radioresistance by Targeting IL8/AKT Signaling

et al. 2015

Molecular Cancer Therapeutics

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Radioresistance poses a major challenge in nasopharyngeal carcinoma (NPC) treatment, but little is known about how miRNA (miR) regulates this phenomenon. In this study, we investigated the function and mechanism of miR-203 in NPC radioresistance, one of downregulated miRs in the radioresistant NPC cells identified by our previous microarray analysis. We observed that miR-203 was frequently downregulated in the radioresistant NPC tissues compared with radiosensitive NPC tissues, and its decrement significantly correlated with NPC radioresistance and poor patient survival, and was an independent predictor for reduced patient survival. In vitro radioresponse assays showed that miR-203 mimic markedly decreased NPC cell radioresistance. In a mouse model, therapeutic administration of miR-203 agomir dramatically sensitized NPC xenografts to irradiation. Mechanistically, we confirmed that IL8 was a direct target of miR-203, and found that reduced miR-203 promoted NPC cell radioresistance by activating IL8/ AKT signaling. Moreover, the levels of IL8 and phospho-AKT were significantly increased in the radioresistant NPC tissues compared with radiosensitive NPC tissues, and negatively associated with miR-203 level. Our data demonstrate that miR-203 is a critical determinant of NPC radioresponse, and its decrement enhances NPC radioresistance through targeting IL8/AKT signaling, highlighting the therapeutic potential of the miR-203/IL8/AKT signaling axis in NPC radiosensitization. Mol Cancer Ther; 14(11); 2653-64. Ó2015 AACR.

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“…HSPB1 overexpression has been correlated with risk in lung cancer [Guo et al, ; Xu et al, ], pancreatic cancer [Heinrich et al, ; Shiota et al, ], prostate cancer [Shiota et al, ] and ovarian cancer [Zhao et al, ], and is involved in interferon pathway activation in HBV‐associated hepatocellular carcinoma [Tong et al, ]. Several studies have demonstrated that HSPB1 is a chemoradiation‐related protein which may promote drug‐resistance and radioresistance in cancer therapy [Zhang et al, ; Xu et al, ; Pang et al, ]. Therefore, HSPB1 may also play a vital role in the genesis and progression of NPC.…”

Section: Discussionmentioning

confidence: 99%

iTRAQ‐Based Quantitative Proteomic Analysis of Nasopharyngeal Carcinoma

Cai

Zeng

Xiang

et al. 2015

J of Cellular Biochemistry

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Nasopharyngeal carcinoma (NPC) is a common disease in the southern provinces of China with a poor prognosis. To better understand the pathogenesis of NPC and identify proteins involved in NPC carcinogenesis, we applied iTRAQ coupled with two-dimensional LC-MS/MS to compare the proteome profiles of NPC tissues and the adjacent non-tumor tissues. We identified 54 proteins with differential expression in NPC and the adjacent non-tumor tissues. The differentially expressed proteins were further determined by RT-PCR and Western blot analysis. In addition, the up-regulation of HSPB1, NPM1 and NCL were determined by immunohistochemistry using tissue microarray. Functionally, we found that siRNA mediated knockdown of NPM1 inhibited the migration and invasion of human NPC CNE1 cell line. In summary, this is the first study on proteome analysis of NPC tissues using an iTRAQ method, and we identified many new differentially expressed proteins which are potential targets for the diagnosis and therapy of NPC.

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Identification of heat shock protein 27 as a radioresistance-related protein in nasopharyngeal carcinoma cells

Abstract: The data suggest that HSP27 is a radioresistant protein in NPC cells, and its upregulation may be involved in the NPC radioresistance.

Cited by 25 publications

References 33 publications

Proteomic Characterization Reveals a Molecular Portrait of Nasopharyngeal Carcinoma Differentiation

Proteomic Characterization Reveals a Molecular Portrait of Nasopharyngeal Carcinoma Differentiation

MiRNA-203 Reduces Nasopharyngeal Carcinoma Radioresistance by Targeting IL8/AKT Signaling

iTRAQ‐Based Quantitative Proteomic Analysis of Nasopharyngeal Carcinoma

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