Identification of Human-Derived Volatile Chemicals That Interfere With Attraction of the Scottish Biting Midge and Their Potential Use as Repellents

Logan, James G.; Seal, N. J.; Cook, James; Stanczyk, Nina M.; Birkett, Michael A.; Clark, S. J.; Gezan, Salvador A.; Wadhams, L. J.; Pickett, John A.; Mordue, A. J.

doi:10.1603/033.046.0205

Cited by 53 publications

(58 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The antenna was placed 0.5 cm from the outlet of this tube. For EAD recordings, we modified the method described by Logan et al (2009). In short, the head of a C. nubeculosus female was separated from the body, and the tip of each antenna was cut.…”

Section: Electrophysiologymentioning

confidence: 99%

“…It is noteworthy that 2-ethyl-1-hexanol is a precursor of the common plasticiser dioctyl phthalate (Thorat et al 1992) and may, therefore, be an artefact or pollutant. However The ketone, 6-methyl-5-hepten-2-one, has also been shown to regulate differential attraction of C. impunctatus (Logan et al 2009) and of the mosquito Aedes egypti (Logan et al 2008) to humans, as well as of the horn fly, Heamatobia irritans, to cattle (Birkett et al 2004). Similar to these studies, we observed attraction, behavioral inhibition or nonpreference depending on the concentration tested.…”

Section: Behavioral Assaymentioning

confidence: 99%

See 1 more Smart Citation

Identification of Cattle-Derived Volatiles that Modulate the Behavioral Response of the Biting Midge Culicoides nubeculosus

et al. 2015

View full text Add to dashboard Cite

Identification of host-derived volatiles is an important step towards the development of novel surveillance and control tools for Culicoides biting midges. In this study, we identified compounds from headspace collections of cattle hair and urine that modulate the behavioral response of Culicoides nubeculosus, a research model species with a similar hostrange as the vectors of Bluetongue disease and Schmallenberg disease in Europe. Combined gas chromatography and electroantennographic detection (GC-EAD) analysis revealed 23 bioactive compounds, of which 17, together with octanal, were evaluated in a two-choice behavioral assay in the presence of CO2. Decanal, 2-phenylethanal, 1-octen-3-ol, 2-ethylhexanol, 3-methylindole, phenol, and 3-ethylphenol elicited attraction of host seeking C. nubeculosus, whereas heptanal, octanal, nonanal, 3-propylphenol, and 4-action to CO2, when compared to CO2 alone. 6-Methyl-5-hepten-2-one, 3-methylphenol, 4-methylphenol, and 4-ethylphenol elicited both attraction and inhibition. The behavioral responses were dependent on the concentration tested. Our results show that cattle-derived odors have the potential to be used for the manipulation of the behavior of Culicoides biting midges.

show abstract

Section: Electrophysiologymentioning

confidence: 99%

Section: Behavioral Assaymentioning

confidence: 99%

Identification of Cattle-Derived Volatiles that Modulate the Behavioral Response of the Biting Midge Culicoides nubeculosus

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Bed bugs are able to detect C 7 -C 10 aldehydes and sulcatone present in human emanates, similar to other blood-feeding insects (Gikonyo et al, 2002;Birkett et al, 2004;Ghaninia et al, 2008;Logan et al, 2008;Logan et al, 2009;Syed and Leal, 2009;Logan et al, 2010). Quantitative differences in the amount of these compounds in headspace volatile extracts collected from a group of volunteers modulate the behavioural response of bed bugs.…”

Section: Discussionmentioning

confidence: 69%

Smelling your way to food: can bed bugs use our odour?

Harraca

Ryne

Birgersson

et al. 2012

Journal of Experimental Biology

View full text Add to dashboard Cite

SUMMARYThe resurgence in developed countries of the common bed bug, Cimex lectularius, has led to a search for new sustainable methods to monitor and control this human ectoparasite. Because of increased resistance to insecticides, traps baited with attractive cues are considered a promising method to be developed into efficient monitoring tools for bed bugs. Despite their potential as attractants, only a few studies have investigated the odorant cues implicated in the attraction of bed bugs to human hosts. In this study, we used aeration extracts from human volunteers to assess the role of olfaction in host searching by bed bugs. By coupled gas chromatography and single sensillum recordings on all the antennal sensilla, we measured the electrophysiological response elicited by the compounds present in our human odour extracts. Only five compounds were clearly detected by the olfactory receptor neurons housed in the smooth-peg sensilla of the bed bugs. We tested the behavioural effect of these extracts in a still-air arena and showed a gradient of repellence linked to the dose, as well as a higher propensity of local search behaviour associated with human odours containing a lower ratio of 6-methyl-5-hepten-2-one to C 7 -C 10 aldehydes. We conclude that human odour alone has a weak influence on the behaviour of C. lectularius and we propose that human kairomones may have a significant impact on bed bug behaviour in combination with heat and carbon dioxide, the only two currently known attractive vertebrate cues used by bed bugs for host seeking.

show abstract

“…[1][2][3][4][5][6][7][8][9][10] The molecules in the cloud penetrate in their cuticular pores and are captured by the olfactory binding proteins (OBP). Next, these molecules are transported to the olfactory receptor neurons (ORN), located in the dendritic membranes at the antenna sensory bristles, driving the mosquito's attention to the potential meal.…”

Section: Introductionmentioning

confidence: 99%

“…Next, these molecules are transported to the olfactory receptor neurons (ORN), located in the dendritic membranes at the antenna sensory bristles, driving the mosquito's attention to the potential meal. 3,9,[11][12][13][14] Studies by Biessmann et al 11 have proved the importance of OBP for smell recognition in the search for food, in mating and other activities. 3,12,14 Among the 60 types of OBP coded in the A. gambiae genoma, the Anopheles gambiae odorant binding protein (AgOBP 1 ) was found in higher levels in the antennas of females when compared to males and its levels were observed to reduce when the females feed on blood.…”

Section: Introductionmentioning

confidence: 99%

Applications of molecular modeling in the design of new insect repellents targeting the odorant binding protein of Anopheles gambiae

Affonso¹,

Guimarães²,

Oliveira³

et al. 2013

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

Mosquitos são responsáveis pela transmissão de várias doenças causadas por vírus, parasitas e helmintos. Considerando o custo e a complexidade do tratamento dessas doenças, o uso de repelentes para proteção contra mosquitos vetores se torna uma alternativa interessante. Nesse contexto, no presente trabalho foram feitos estudos por ancoramento e dinâmica molecular (DM) de potenciais ligantes da proteína odorante do mosquito Anopheles gambiae (AgOBP 1 ), o principal vetor da malária. Os modos de ligação na AgOBP 1 de moléculas com conhecida atividade atrativa e dos principais componentes do óleo do cravo da índia (Syzygium aromaticum), com potencial atividade repelente, foram comparados com o conhecido repelente N,N-dietil-3-metilbenzamida (DEET). Os resultados sugerem que o acetato de eugenila é um repelente melhor do que o DEET e também revelam as principais características do sítio de ligação da AgOBP 1 importantes para o planejamento de novos e mais eficientes repelentes.Mosquitoes are responsible for conveying various diseases caused by viruses, parasites and helminthes. Considering the cost and complexity of the treatment of these diseases, the use of repellents for protection from the mosquito vectors becomes an interesting alternative. In the present work, docking and molecular dynamics (MD) studies were performed on potential ligands to the odorant binding protein of the mosquito Anopheles gambiae (AgOBP 1 ), the main vector of malaria. The binding modes on AgOBP 1 of molecules with known attractive activities and the main components of the oil of indian clove (Syzygium aromaticum) with potential repellent activities were compared to the known repellent N, . Results suggest the eugenyl acetate as a better repellent than DEET and also reveal the main features of the binding site of AgOBP 1 important to the design of new and more efficient repellents.

show abstract

Identification of Human-Derived Volatile Chemicals That Interfere With Attraction of the Scottish Biting Midge and Their Potential Use as Repellents

Cited by 53 publications

References 31 publications

Identification of Cattle-Derived Volatiles that Modulate the Behavioral Response of the Biting Midge Culicoides nubeculosus

Identification of Cattle-Derived Volatiles that Modulate the Behavioral Response of the Biting Midge Culicoides nubeculosus

Smelling your way to food: can bed bugs use our odour?

Applications of molecular modeling in the design of new insect repellents targeting the odorant binding protein of Anopheles gambiae

Contact Info

Product

Resources

About