Identification of
            <i>Bacillus</i>
            Spores by Matrix-Assisted Laser Desorption Ionization–Mass Spectrometry

Hathout, Yetrib; Demirev, Plamen A.; Ho, Yen‐Peng; Bundy, Jonathan; Ryzhov, Victor; Sapp, Lisa; Stutler, James; Jackman, Joany; Fenselau, Catherine

doi:10.1128/aem.65.10.4313-4319.1999

Cited by 116 publications

(74 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…molecules in the lower mass range. 27 DHB alone was previously recognized to be efficient for the detection of lower mass secondary metabolites such as cyclic lipopeptides, 6 whereas the DHBs-mixture is known to be efficient for proteins above 25 kDa in terms of sensitivity and resolution. 28 Whole-cell MALDI-TOFMS of B. subtilis ATCC 6633 grown on Landy agar plates .…”

Section: Whole-cell Sample Preparationmentioning

confidence: 99%

“…Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) 1 has been used for biomarker profiling to type pathogenic and nonpathogenic microorganisms [2][3][4][5][6] as well as metabolic profiling in crude culture supernatants. 7,8 Even catalytic activities were monitored within bacterial cultures without time-consuming isolation procedures.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Whole‐cell matrix‐assisted laser desorption/ionization mass spectrometry for rapid identification of bacteriocin/lantibiotic‐producing bacteria

Stein

2008

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

The potential of whole-cell matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) for the discovery of microorganisms that produce lantibiotics and/or bacteriocins in the mass range 3-5 kDa is demonstrated. Cells of the antibiotic-producing model strain Bacillus subtilis ATCC 6633 were grown on Landy agar plates, and prepared with a 9:1 mixture of 2,5-dihydroxybenzoic acid and 2-hydroxy-5-methoxybenzoic acid (DHBs) as matrix using the bottom-layer method. Time-course analyses showed that cells grown for 1-2 days on agar plates gave the best results in terms of intensity of the bacteriocin/lantibiotic signals. Even after storage of matrix-crystal-embedded cells for several days high-resolution spectra (>10,000) could be obtained. To verify the applicability of this technique, different Bacillus wild-type and gene deletion mutant cells were analyzed. In most of the cases the known antibiotic phenotype of the producer could be corroborated by whole-cell MALDI-TOFMS, particularly for the lantibiotics subtilin and ericin. The preparation recipe for whole-cell analysis by MALDI-TOF was also highly sensitive for hydrophobic lipopeptide antibiotics like surfactin, mycosubtilin, fengycin, and bacillomycin in the lower mass range (1-1.5 kDa).

show abstract

Section: Whole-cell Sample Preparationmentioning

confidence: 99%

mentioning

confidence: 99%

Whole‐cell matrix‐assisted laser desorption/ionization mass spectrometry for rapid identification of bacteriocin/lantibiotic‐producing bacteria

Stein

2008

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

show abstract

“…Several reports have shown the effectiveness of matrixassisted laser desorption/ionization time-of-flight MS (MALDI-TOF MS) to identify small molecule biomarkers that distinguish and characterize different Bacillus strains and species (Hathout et al, 1999;Gebhardt et al, 2002;Bonmatin et al, 2003;Madonna et al, 2003;Vater et al, 2003;Pittenauer et al, 2006). Most identified Bacillus biomarkers are cyclic peptides and lipopeptides, typically in the mass range of 800-5000 Da, and several have distinct antimicrobial properties.…”

Section: Introductionmentioning

confidence: 99%

Mass spectrometric analysis of lipopeptides from Bacillus strains isolated from diverse geographical locations

Price

Rooney

Swezey

et al. 2007

FEMS Microbiology Letters

104

View full text Add to dashboard Cite

Matrix-assisted laser desorption/ionization time-of-flight MS (MALDI-TOF MS) has been applied to characterize lipopeptide biomarkers from 54 different strains of Bacillis from most taxa within the Bacillis subtilis-Bacillis licheniformis clade, isolated from seven geographic locations on five continents. Even the most narrowly defined taxa are diverse in terms of the lipopeptide profiles. Many strains produce previously identified compounds with known antimicrobial properties (e.g. polymyxins and bacitracins), whereas other compounds represent novel classes that were hitherto unknown. Of particular interest is the novel 942/958 Da biomarkers produced by B. s. spizizeni desert strains and several type strains.

show abstract

“…Although an in-depth discussion of the technology is not presented here, it is clear that mass spectrometry methods allow discrimination of bacteria, including Francisella tularensis subspecies and other organisms of bioterrorism interest (71,72). These methods have also been used to identify Burkholderia species and Bacillus spores (73,74). A significant issue with MALDI-TOF mass spectrometry is the misidentification of select agents as nonbioterrorism agents because the database does not include select agents.…”

Section: New Technology and Bioterrorism Preparednessmentioning

confidence: 99%

Bioterrorism and the Role of the Clinical Microbiology Laboratory

Wagar

2016

Clin Microbiol Rev

View full text Add to dashboard Cite

SUMMARYRegular review of the management of bioterrorism is essential for maintaining readiness for these sporadically occurring events. This review provides an overview of the history of biological disasters and bioterrorism. I also discuss the recent recategorization of tier 1 agents by the U.S. Department of Health and Human Services, the Laboratory Response Network (LRN), and specific training and readiness processes and programs, such as the College of American Pathologists (CAP) Laboratory Preparedness Exercise (LPX). LPX examined the management of cultivable bacterial vaccine and attenuated strains of tier 1 agents or close mimics. In the LPX program, participating laboratories showed improvement in the level of diagnosis required and referral of isolates to an appropriate reference laboratory. Agents which proved difficult to manage in sentinel laboratories included the more fastidious Gram-negative organisms, especiallyFrancisella tularensisandBurkholderiaspp. The recent Ebola hemorrhagic fever epidemic provided a check on LRN safety processes. Specific guidelines and recommendations for laboratory safety and risk assessment in the clinical microbiology are explored so that sentinel laboratories can better prepare for the next biological disaster.

show abstract

Identification of Bacillus Spores by Matrix-Assisted Laser Desorption Ionization–Mass Spectrometry

Cited by 116 publications

References 12 publications

Whole‐cell matrix‐assisted laser desorption/ionization mass spectrometry for rapid identification of bacteriocin/lantibiotic‐producing bacteria

Whole‐cell matrix‐assisted laser desorption/ionization mass spectrometry for rapid identification of bacteriocin/lantibiotic‐producing bacteria

Mass spectrometric analysis of lipopeptides from Bacillus strains isolated from diverse geographical locations

Bioterrorism and the Role of the Clinical Microbiology Laboratory

Contact Info

Product

Resources

About