Identification of
            <i>Brucella abortus</i>
            Antibodies in Cattle Serum by Single Radial Diffusion

Brucellosis is a disease caused by members of the genus Brucella that affects animals and humans. The species that infects cattle most often is Brucella abortus, but cattle infections by Brucella melitensis are not rare in areas where there is contact with infected sheep and goats (63, 64). Both B. abortus and B. melitensis are termed smooth (S) because they bear a S-type lipopolysaccharide (S-LPS). Many serological tests have been proposed for the diagnosis of brucellosis caused by S brucellae, and they can be broadly classified as those detecting antibodies to the S-LPS and those detecting antibodies to proteins (21,45). The former tests use either suspensions of S brucellae as antigens (3) or S-LPS extracts. The Rose Bengal test (RBT) and the complement fixation test (CFT) belong to the first group, and are recommended by the Office International des Épizooties for international trade (4). In addition, indirect enzyme-linked immunosorbent assays (ELISA) using S-LPS extracts or its O-chain have been extensively studied (47) and may replace the RBT and CFT. S-LPS tests are the most sensitive for detecting cattle brucellosis, but they may yield false positive results for cattle vaccinated with B. abortus

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Efficacy of Several Serological Tests and Antigens for Diagnosis of Bovine Brucellosis in the Presence of False-Positive Serological Results Due to Yersinia enterocolitica O:9

Muñoz

Marín

Monreal

et al. 2005

Clin Vaccine Immunol

134

107

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Antibody and delayed-type hypersensitivity responses to Ochrobactrum anthropi cytosolic and outer membrane antigens in infections by smooth and rough Brucella spp

Velasco

Díaz

Grilló

et al. 1997

Clin Diagn Lab Immunol

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Immunological cross-reactions between Brucella spp. and Ochrobactrum anthropi were investigated in animals and humans naturally infected by Brucella spp. and in experimentally infected rams (Brucella ovis infected), rabbits (Brucella melitensis infected), and mice (B. melitensis and Brucella abortus infected). In the animals tested, O. anthropi cytosolic proteins evoked a delayed-type hypersensitivity reaction of a frequency and intensity similar to that observed with B. melitensis brucellin. O. anthropi cytosolic proteins also reacted in gel precipitation tests with antibodies in sera from Brucella natural hosts with a frequency similar to that observed with B. melitensis proteins, and absorption experiments and immunoblotting showed antibodies to both Brucella-specific proteins and proteins common to Brucella and O. anthropi. No antibodies to O. anthropi cytosolic proteins were detected in the sera of Brucella-free hosts. Immunoblotting with sera of Brucella-infected sheep and goats showed immunoglobulin G (IgG) to Brucella group 3 outer membrane proteins and to O. anthropi proteins of similar molecular weight. No IgG to the O-specific polysaccharide of O. anthropi lipopolysaccharide was detected in the sera of Brucella-infected hosts. The sera of sheep, goats, and rabbits infected with B. melitensis contained IgG to O. anthropi rough lipopolysaccharide and lipid A, and B. ovis and O. anthropi rough lipopolysaccharides showed equal reactivities with IgG in the sera of B. ovis-infected rams. The findings show that the immunoresponse of Brucella-infected hosts to protein antigens is not necessarily specific for brucellae and suggest that the presence of O. anthropi or some related bacteria explains the previously described reactivities to Brucella rough lipopolysaccharide and outer membrane proteins in healthy animals.

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Radial immunodiffusion test with a Brucella polysaccharide antigen for differentiating infected from vaccinated cattle

Díaz¹,

Garatea²,

Jones³

et al. 1979

J Clin Microbiol

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Identification of Brucella abortus Antibodies in Cattle Serum by Single Radial Diffusion

Abstract: Single radial diffusion combined with the Rose Bengal test permitted rapid identification of all of the Brucella abortus -infected cattle in a study group of 689 animals.

Cited by 4 publications

References 8 publications

Efficacy of Several Serological Tests and Antigens for Diagnosis of Bovine Brucellosis in the Presence of False-Positive Serological Results Due to Yersinia enterocolitica O:9

Efficacy of Several Serological Tests and Antigens for Diagnosis of Bovine Brucellosis in the Presence of False-Positive Serological Results Due to Yersinia enterocolitica O:9

Antibody and delayed-type hypersensitivity responses to Ochrobactrum anthropi cytosolic and outer membrane antigens in infections by smooth and rough Brucella spp

Radial immunodiffusion test with a Brucella polysaccharide antigen for differentiating infected from vaccinated cattle

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