Identification of<i>Haemophilus Influenzae</i>

Newman, Robert B.

doi:10.3109/10408367209151323

Cited by 5 publications

(2 citation statements)

References 190 publications

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“…The LA requires at least an 18-h broth culture supernatant (10). If performed on a glass slide it only takes 2 min.…”

Section: Resultsmentioning

confidence: 99%

“…The Quellung reaction (capsular swelling) was used later as a simple typing method (1,2,12). Recently, four other techniques, countercurrent immunoelectrophoresis (CIE) (3,4), the latex agglutination test (LA) (10,11), antiserum agar (culture plates containing specific antibody) (8), and immunofluorescence (4, 6, 13, 15) have been employed as alternative methods for typing H. influenza. This study was designed to determine whether the slide agglutination test, Quellung reaction, CIE, LA, and antiserum agar gave comparable results when used to type nasopharyngeal isolates of H. influenza and to examine the advantages and disadvantages of using each of these tests for this purpose.…”

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confidence: 99%

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Methods for serotyping nasopharyngeal isolates of Haemophilus influenzae: slide agglutination, Quellung reaction, countercurrent immunoelectrophoresis, latex agglutination, and antiserum agar

Ingram¹,

Collier²,

Pendergrass³

et al. 1979

J Clin Microbiol

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Nasopharyngeal isolates of H. influenzae were typed by the slide agglutination test, the Quelling reaction, the latex agglutination test, countercurrent immunoelectrophoresis, and the antiserum agar test. These tests gave essentially comparable results, with countercurrent immunoelectrophoresis and latex agglutination being slightly more sensitive. Cross-reactive problems encountered with latex agglutination and the expense of performing countercurrent immunoelectrophoresis or the antiserum agar test made these tests less practical than the slide agglutination test to identify single strains that were already isolated. The Quellung reaction and slide agglutination were the most rapid tests used to type an organism. For mass screening of multiple samples, countercurrent immunoelectrophoresis was the simplest technique. The antiserum agar test was slow but was the best technique to screen nasopharyngeal swab cultures to identify the presence of any encapsulated strains in the mixed flora. Whether any of the above techniques were as sensitive as the immunofluorescence test was not evaluated in this study.

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“…The LA requires at least an 18-h broth culture supernatant (10). If performed on a glass slide it only takes 2 min.…”

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Methods for serotyping nasopharyngeal isolates of Haemophilus influenzae: slide agglutination, Quellung reaction, countercurrent immunoelectrophoresis, latex agglutination, and antiserum agar

Ingram¹,

Collier²,

Pendergrass³

et al. 1979

J Clin Microbiol

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Ampicillin-Resistant Haemophilus influenzae Colonizing Ambulatory Children

Scheifele¹,

Fussell²

1981

Am J Dis Child

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Antimicrobial Susceptibility of Haemophilus influenzae

Thornsberry

Kirven

1974

Antimicrob Agents Chemother

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Selected clinical isolates of Haemophilus influenzae were tested for their susceptibility to seven antibiotics by a microtiter broth dilution and an agar diffusion method. Eleven of 40 strains tested were resistant to ampicillin, the drug of choice, by both methods. All the strains tested were susceptible to chloramphenicol, and all but one were susceptible to tetracycline. Of the other four antibiotics tested, the ranges of minimal inhibitory concentrations (MICs) were: 0.5 to 4 pg of gentamicin per ml, 0.5 to 4 ,ug of erythromycin per ml, and 2 to 16 pg of clindamycin per ml. MICs of ampicillin for both the susceptible and resistant strains were markedly affected by inoculum concentration. The ampicillin MICs of the resistant strains were also affected by the time of incubation.

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Identification ofHaemophilus Influenzae

Cited by 5 publications

References 190 publications

Methods for serotyping nasopharyngeal isolates of Haemophilus influenzae: slide agglutination, Quellung reaction, countercurrent immunoelectrophoresis, latex agglutination, and antiserum agar

Methods for serotyping nasopharyngeal isolates of Haemophilus influenzae: slide agglutination, Quellung reaction, countercurrent immunoelectrophoresis, latex agglutination, and antiserum agar

Ampicillin-Resistant Haemophilus influenzae Colonizing Ambulatory Children

Antimicrobial Susceptibility of Haemophilus influenzae

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