IDENTIFICATION OF<i>MYCOBACTERIUM GENAVENSE</i>IN A DIANA MONKEY (<i>CERCOPITHECUS DIANA</i>) BY POLYMERASE CHAIN REACTION AND HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

Kelly, Kathleen M.; Wack, Allison N.; Bradway, Daniel S.; Simons, Brian W.; Bronson, Ellen; Osterhout, Gerard; Parrish, Nicole; Montali, Richard J.

doi:10.1638/2013-0246r2.1

Cited by 5 publications

(2 citation statements)

References 26 publications

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“…To identify the agent, DNA was extracted from the formalin-fixed, paraffin-embedded tissues (QIAamp DNA mini-kit, Qiagen, Valencia, CA), amplified using universal Mycobacterium genus-specific primers for the 16S-23S ribosomal RNA ITS gene, directly sequenced, and analyzed as previously described. 11,15 ITS gene sequence alignment revealed 100% sequence identity between the Atlantic guitarfish sample sequence and banked M. chelonae sequences (DQ866772 and AJ294537). The Atlantic guitarfish sequence was <94% similar to the ITS sequences from the next most closely related species, Mycobacterium salmoniphilum (DQ866764).…”

Section: Casementioning

confidence: 99%

“…There were large numbers of organisms with relatively mild inflammatory responses, mirroring the course and morphologic features of mycobacteriosis described in sygnathids, 3,4 whereas in teleosts the disease tends to be chronic and granulomatous. 7,11 Fish can display a wide range of clinical signs, from no outward signs of illness to severe changes leading to death. Unlike these 2 cases, chronic granulomatous disease similar to what is described in teleosts 8,14 was observed in an epaulette shark with M. avium 10 and an Atlantic guitarfish with M. chelonae found in the spleen.…”

Section: Casementioning

confidence: 99%

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Acute disseminated mycobacteriosis in captive Atlantic guitarfish (Rhinobatos lentiginosus)

et al. 2017

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An adult female captive-born Atlantic guitarfish ( Rhinobatos lentiginosus) was found acutely moribund on exhibit and died soon after presentation. Abnormalities on autopsy were focal cutaneous erythema on the tail, a small liver, many variably sized friable ovarian follicles, and coelomic effusion. Histologic examination revealed systemic bacterial embolization, and yolk coelomitis with minimal associated inflammation and some mineralization. Bacterial culture of blood and coelomic effusion grew a rapidly growing Mycobacterium species that was further identified as Mycobacterium chelonae by PCR amplification and sequencing of the RNA polymerase subunit beta ( rpoB) gene of isolated genomic DNA. Concurrent reproductive disease may have caused immunosuppression, thus predisposing to the mycobacterial infection. At another institution, an adult male wild-caught Atlantic guitarfish was found dead on exhibit with no premonitory signs. Abnormalities on autopsy were a thin body condition, small liver, and coelomic effusion. Histologic examination revealed acute mycobacterial septicemia. M. chelonae was also identified in this fish by PCR amplification and sequencing. Mycobacteriosis has rarely been reported in captive elasmobranchs. Guitarfish may have greater susceptibility to mycobacteriosis than other elasmobranchs, and acute and chronic manifestations of the disease may exist in this species.

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Section: Casementioning

confidence: 99%

Section: Casementioning

confidence: 99%

Acute disseminated mycobacteriosis in captive Atlantic guitarfish (Rhinobatos lentiginosus)

et al. 2017

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Mycobacterium genavense detection in a blood smear from a domestic ferret

Fonseca,

Silveira,

Duarte

et al. 2024

Vet Record Case Reports

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The domestic ferret is sensitive to mycobacterial infections, which are likely underdiagnosed. There are limited reports of therapy for mycobacteriosis in ferrets and most have failed. A ferret in poor body condition revealed marked organomegaly on diagnostic imaging. The complete blood count revealed a mild pseudoeosinophilia, which might be attributed to negatively stained bacilli phagocytised by monocytes observed in the blood smear. Ziehl–Neelsen stain was performed and showed acid‐fast bacilli within monocytes. Fine‐needle aspirates of the spleen and liver revealed the same finding. Mycobacterium genavense was identified by PCR and DNA sequencing from peripheral blood. Treatment with rifampicin, clarithromycin and enrofloxacin was initiated. The disseminated mycobacteriosis was suspected from the observation of bacilli on the blood smear. After 16 months, the ferret is still undergoing treatment. To prevent recurrence, continued monitoring of drug toxicity and effective therapy will be maintained even after the resolution of clinical signs (mostly organomegaly).

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