Identification of Pseudomonas syringae pv. actinidiae as Causal Agent of Bacterial Canker of Yellow Kiwifruit (Actinidia chinensis Planchon) in Central Italy

Ferrante, Patrizia; Scortichini, M.

doi:10.1111/j.1439-0434.2009.01550.x

Cited by 102 publications

(91 citation statements)

References 8 publications

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“…actinidiae, which causes kiwifruit canker, is another devastating plant pathogen and has received significant attention in recent years. [3][4][5][6] Thus, there has been considerable interest in development of bactericides for Ralstonia solanacearum and Pseudomonas syringae pv. actinidiae.…”

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confidence: 99%

Synthesis and Antibacterial Activities of Yanglingmycin Analogues

Dan

Tan

et al. 2015

Chem. Pharm. Bull.

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The synthesis of Yanglingmycin and its enantiomer, along with eighteen Yanglingmycin analogues is reported. The structures were confirmed mainly by analyses of NMR spectral data. Antibacterial activity assays showed that Yanglingmycin and some of its analogues exhibited significant antibacterial activities against two important agricultural pathogenic bacteria, Ralstonia solanacearum and Pseudomonas syringae pv. actinidiae, with minimum inhibitory concentration (MIC) values ranging from 3.91 to 15.62 µg/mL. The antibacterial activities exhibited by Yanglingmycin and its analogues are promising, suggesting potential in the development of compounds for novel bactericides.Key words Yanglingmycin; structural analogue; substituted cyclic azole; antibacterial activity Bacterial diseases pose a major threat to agricultural production worldwide. For example, Ralstonia solanacearum, which causes plant bacterial wilt, is one of the most destructive plant pathogens, and affects many regions of the world. 1)It is estimated that R. solanacearum is responsible for US$ 1 billion in losses each year.2) Pseudomonas syringae pv. actinidiae, which causes kiwifruit canker, is another devastating plant pathogen and has received significant attention in recent years.3-6) Thus, there has been considerable interest in development of bactericides for Ralstonia solanacearum and Pseudomonas syringae pv. actinidiae. Unfortunately, there have been few reports of effective bactericides against these pathogens. In the process of screening new agricultural antibiotics, we report the synthesis a new broad spectrum antibiotic, Yanglingmycin (Fig. 1), which was found to be effective against the previously mentioned pathogens. 7) Although Yanglingmycin can be isolated from the fermentation broth of Streptomyces djakartensis, the yield is too low to conduct experiments to determine the mechanism of bactericidal action. In order to investigate the bactericidal activities of Yanglingmycin and its analogues, Yanglingmycin and its analogues were designed and synthesized. In this paper, Yanglingmycin and its enantiomer and eighteen Yanglingmycin analogues were synthesized and their antibacterial activities against Ralstonia solanacearum and Pseudomonas syringae pv. actinidiae are reported. ResultsChemistry Chart 1 describes the synthesis of all target compounds. Benzonitrile, 2-hydroxybenzonitrile, and 4-hydroxybenzonitrile were used as starting material for the Pinner reaction to obtain the corresponding methyl benzimidate hydrochlorides, 8) which were then reacted with each the following compounds: L-serine methyl ester hydrochloride, D-serine methyl ester hydrochloride, L-cysteine methyl ester hydrochloride, D-cysteine methyl ester hydrochloride, L-2,3-diaminopropionic acid methyl ester hydrochloride, and D-2,3-diaminopropionic acid methyl ester hydrochloride.9,10) Amino acid methyl ester hydrochlorides were obtained by reacting the corresponding amino acids with AcCl in MeOH.11) The products were reduced by LiAlH 4 to obtain Yanglingmycin and its an...

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Synthesis and Antibacterial Activities of Yanglingmycin Analogues

Dan

Tan

et al. 2015

Chem. Pharm. Bull.

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“…During spring 2008, however, P. syringae pv. actinidiae was observed in some A. chinensis orchards in central Italy and caused death of kiwifruit plants (Balestra et al, 2009;Ferrante and Scortichini, 2009), and similar outbreaks were found on A. deliciosa . A recent outbreak of bacterial canker is devastating the Italian kiwifruit industry.…”

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Occurrence of a New Type of Pseudomonas syringae pv. actinidiae Strain of Bacterial Canker on Kiwifruit in Korea

Koh¹,

Kim²,

Koh³

et al. 2012

The Plant Pathology Journal

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Pseudomonas syringae pv. actinidiae strains, the causal agents of bacterial canker on kiwifruit, were isolated from Korea and Italy in 2011. Among 87 isolates, a total of six representative strains, three from Korea and three from Italy, were identified on the basis of biochemical and physiological tests. Identities were confirmed by PCR using P. syringae pv. actinidiae-specific primers PsaF1/R2, which amplified a 280-bp DNA fragment. The strains isolated from Korea in this study displayed BOX-PCR patterns similar to those isolated from Italy but different from those isolated previously in Korea or the pathotype P. syringae pv. actinidiae strain. The effector hopA1 and hopH1 genes, which are known to be present in strains isolated recently from France and Italy, were also present in P. syringae pv. actinidiae strains, SYS1, SYS2 and SYS4, isolated from Korea in this work. However, no amplicons of the expected size were obtained from strains previously isolated from Korea and Japan. In addition, the Korean strains isolated in this work belonged to haplotype I for the cts gene identical to those strains isolated from recent outbreaks in Italy. These results suggest that P. syringae pv. actinidiae strains isolated from Korea and examined in this work are a new type of strain similar to those found from recent outbreaks in Italy. This is the first report on the occurrence of cts haplotype I strains of P. syringae pv. actinidiae affecting kiwifruit plants in Korea.

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“…The causal agent of bacterial canker is Pseudomonas syringae pv. actinidiae, which affects the kiwifruit species Actinidia deliciosa and Actinidia chinensis (Serizawa et al 1989;Scortichini 1994;Koh & Nou 2002;Ferrante & Scortichini 2009;Koh et al 2010;Vanneste et al 2011). Canker disease in kiwifruit caused by P. syringae pv.…”

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confidence: 99%

Pectobacterium carotovorumsubsp.actinidiaesubsp. nov., a new bacterial pathogen causing canker-like symptoms in yellow kiwifruit,Actinidia chinensis

Koh

et al. 2012

New Zealand Journal of Crop and Horticultural Science

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Identification of Pseudomonas syringae pv. actinidiae as Causal Agent of Bacterial Canker of Yellow Kiwifruit (Actinidia chinensis Planchon) in Central Italy

Cited by 102 publications

References 8 publications

Synthesis and Antibacterial Activities of Yanglingmycin Analogues

Synthesis and Antibacterial Activities of Yanglingmycin Analogues

Occurrence of a New Type of Pseudomonas syringae pv. actinidiae Strain of Bacterial Canker on Kiwifruit in Korea

Pectobacterium carotovorumsubsp.actinidiaesubsp. nov., a new bacterial pathogen causing canker-like symptoms in yellow kiwifruit,Actinidia chinensis

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