Identification of inter simple sequence repeat (ISSR) markers associated with seed size in wheat

Ammiraju, Jetty S.S.; Dholakia, Bhushan B.; Santra, Dipak K.; Singh, Harinder; Lagu, M. D.; Tamhankar, Shubhada; Dhaliwal, H. S.; Rao, V. S.; Gupta, Vidya S.; Ranjekar, P. K.

doi:10.1007/s001220051703

Cited by 106 publications

(54 citation statements)

References 37 publications

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“…Moreover, the SSR markers of Xgwm210, Xgwm296, and Xgwm455, were detected to be polymorphic and linked to the leaf rust resistance gene Lr39, of which Xgwm210 was the closest marker mapped 10.7 cm from Lr39 [89]. Ammiraju et al [90] found four intersimple sequence repeat (ISSR) markers, UBC8181000, UBC842600, UBC8431200, and UBC814750, controlling seed size in wheat, which can be measured indirectly by 1000-kernel weight (TKW). These markers showed a signiication association with gene efects of 84.662.92, and 2.61%, contributing a total of 31% of the phenotypic variance in seed size.…”

Section: Simple Sequence Repeat (Ssr) and Intersimple Sequence Repeatmentioning

confidence: 99%

Past, Present and Future Molecular Approaches to Improve Yield in Wheat

Kim¹,

Kim²,

Jang³

2017

Wheat Improvement, Management and Utilization

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This chapter addresses the development and use of molecular markers for yield enhancement in wheat. Since their key goal for breeding is to maximize yield, extensive eforts have been made toward the improvement of yield. Agronomic traits related to yield, yield-related, disease resistance, and abiotic stresses are considered to be quantitative traits (QTLs), also known as complex traits, because they are controlled by numerous genes and are afected by environmental factors. Researchers have been studying such traits in the past decades for the development of molecular markers which can be used in various wheat breeding studies mainly involving restriction fragment length polymorphism (RFLP), simple sequence repeat (SSR), single nucleotide polymorphism (SNP), random ampliied polymorphic DNA (RAPD), and ampliied fragment length polymorphism (AFLP). Furthermore, the advent of next-generation sequencing (NGS) has accelerated the discovery of agronomically important genes. All of the technologies have enabled great advances for increasing the productivity of wheat. Here, the past history of irst-generation sequencing, present status of second-generation sequencing, and future potential of translational genomics linked to the yield will be discussed.

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Section: Simple Sequence Repeat (Ssr) and Intersimple Sequence Repeatmentioning

confidence: 99%

Past, Present and Future Molecular Approaches to Improve Yield in Wheat

Kim¹,

Kim²,

Jang³

2017

Wheat Improvement, Management and Utilization

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“…Sixteen Iraqi varieties were screened with ISSR [10], SRAP, and RAPD markers (kits K, L, M and N) (Operon Technology, Alameda, CA).…”

Section: Primer Selectionmentioning

confidence: 99%

Estimation of the Degree of Diversity for Some Iraqi Wheat Varieties through ISSR, SRAP and RAPD Markers

Al-Kaab

Hamdalla

Dweikat

et al. 2016

AJEA

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DNA-based molecular markers such as Inter Simple Sequence Repeat (ISSR), Sequence-Related Amplified polymorphism (SRAP) and Random Amplified Polymorphic DNA (RAPD) were used in this study to examine the genetic differences among sixteen Iraqi wheat varieties. Seventy three primers out of 177 were reproducible and showed clear amplified bands. The degree of genetic diversity, Polymorphism information content (PIC) and resolving power (RP) were estimated. All the studied molecular markers were informative and showed good ability to classify and distinguish 16 wheat varieties. Total number of polymorphic bands is 134, 221 and 55 for ISSR, SRAP and RAPD respectively. PIC and RP values were 0.259, 0.264 and 0.262 and 9.06, 7.87 and 2.78 Original Research ArticleThis document is published under a Creative Commons/Open Access license. Al-Kaab et al.; AJEA, 11(3): 1-11, 2016; Article no.AJEA.20371 2 respectively. It seems that SRAP marker was the most optimal technique for characterization the of Iraqi wheat varieties. The most efficient primers were Ne ISSR 7 for ISSR; Me4-Em3 and Me4-Em9 primers combination for SRAP; Operon primers K14, K15 and K19 for RAPD. The results of this study can be used to as starting point of the data base for Iraqi wheat varieties to be used in the future development of new varieties in a breeding programs.

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“…ISSR-PCR is a genotyping technique based on variation found in the regions between microsatellites. It has been used in genetic fingerprinting (Blair et al, 1999), gene tagging (Ammiraju et al, 2001), detection of clonal variation (Leroy and Leon, 2000), cultivar identification (Wang et al, 2009), phylogenetic analysis (Gupta et al, 2008), detection of genomic instability (Anderson et al, 2001), and assessment of hybridization (Wolfe et al, 1998) in many plant and animal species.…”

Section: Introductionmentioning

confidence: 99%

Growth, volatile oil production and genetic study of some fennel cultivars under different compost levels in sandy soil

El-Leel¹,

Yousef²

2017

IJBB

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Abstract:The present work was conducted during the two successive seasons of 2011/2012 and 2012/2013 at the experimental farm of ElQuassassin Hort. Res. Station, Ismailia Governorate, and Biotechnology Laboratory, Horticulture Research Institute, Agricultural Research Centre, Egypt. The aim of this study was to investigate the effect of three compost fertilizer levels 4, 6 and 8 ton per Fadden using five cultivars of bitter fennel on growth, fruits yield and volatile oil production of fennel (Foeniculum vulgare Mill). These cultivars were Netherland, Indian, Azoricum, German and Local fennel. Several trails were studied including growth and yield production, biochemical (the volatile oil) and molecular genetic (RAPD-and ISSR-PCR) characteristics under Egyptian sandy soil. The results showed that increasing compost level progressively (form 4 to 8 ton/ Fed) and significantly increased the values of such parameters. Azoricum cultivar was superior to other cultivars under study, as it showed the best growth in terms, fruits yield, fruit volatile oil (%) and volatile oil production per plant and Fadden. The main compounds in all fennel volatile oils were: Anethole, Estragole, Fenchone and Limonene. The highest percentage of Anethole found in German cultivar, while the lowest percentage found in Local cultivar, where the highest percentage of Estragole (= Methyl chavicol ) compound undesirable found in Local cultivar, while the lowest percentage found in Netherland and German cultivars. Random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) molecular fingerprinting markers were employed as genetic markers for the assay of the genetic relationship of five fennel cultivars.In RAPD analysis, 10 selected primers displayed a total of 98 amplified fragments, in which 60 (61.22%) were polymorphic fragments. Thirty-one out of 98 RAPD-PCR fragments were found to be useful as cultivar-specific markers. The largest number of RAPD-PCR markers was scored for Indian variety (68 markers), while the lowest (49 markers) was scored for Netherland variety. In the meantime, the highest number of RAPD-PCR cultivar-specific markers was generated by primer OP-C04 (7 markers), while the lowest number of RAPD-PCR specific markers (1 markers) was generated by primers OP-A13 and OP-B04. In ISSR analysis, 4 of the tested ISSR primers generated variable banding patterns. A total of 26 out of 34 ISSR fragments were polymorphic. Eleven DNA amplified fragments were considered as cultivar-specific markers. The cultivars distribution on the consensus tree according to the banding patterns of RAPD differed from that based on ISSR. This may be due to the possibility that each technique of amplified different parts of the genome. Therefore, it would be useful to use a combination of the banding patterns of the two technique in order to use more segments sites of the genome that verify the validity of the consensus tree

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Identification of inter simple sequence repeat (ISSR) markers associated with seed size in wheat

Cited by 106 publications

References 37 publications

Past, Present and Future Molecular Approaches to Improve Yield in Wheat

Past, Present and Future Molecular Approaches to Improve Yield in Wheat

Estimation of the Degree of Diversity for Some Iraqi Wheat Varieties through ISSR, SRAP and RAPD Markers

Growth, volatile oil production and genetic study of some fennel cultivars under different compost levels in sandy soil

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