Identification of interacting proteins of maize mosaic virus glycoprotein in its vector, Peregrinus maidis

Abstract

Rhabdovirus glycoproteins (G) serve multifunctional roles in virus entry, assembly, and exit from animal cells. We hypothesize that maize mosaic virus (MMV) G is required for invasion, infection, and spread in Peregrinus maidis, the planthopper vector. Using a membrane-based yeast two-hybrid assay, we identified 125 P. maidis proteins that physically interacted with MMV G, of which 68% matched proteins with known functions in endocytosis, vesicle-mediated transport, protein synthesis and turnover, nuclear import/export, metabolism and host defense. Physical interaction networks among conserved proteins indicated a possible cellular coordination of processes associated with MMV G translation, protein folding and trafficking. Non-annotated proteins contained predicted functional sites, including a diverse array of ligand binding sites. Cyclophilin A and apolipophorin III co-immunoprecipitated with MMV G, and each showed different patterns of co-localization with G in insect cells. This study describes the first protein interactome for a rhabdovirus spike protein and insect vector.

Rescue of the first alphanucleorhabdovirus entirely from cloned complementary DNA: An efficient vector for systemic expression of foreign genes in maize and insect vectors

“…The major advantage of rhabdoviruses is that they are able to replicate in both plants and insect vectors, providing a convenient and highly efficient approach for transmission of gene editing reagents to multiple hosts through virus infection and insect transmission. Previous studies of MMV transmission biology, molecular interactions between P. maidis and the virus, and genome editing in the vector make this system a good platform for further characterizing rhabdovirus–vector interactions (Barandoc‐Alviar et al, 2016 , 2022 ; Klobasa et al, 2021 ). Other rhabdoviruses, BYSMV (Gao et al, 2019 ) and SYNV (Ma et al, 2020 ), can express multiple guide RNAs and Cas nucleases.…”

Rescue of the first Alphanucleorhabdovirus entirely from cloned complementary DNA: an efficient vector for systemic expression of foreign genes in maize and insect vectors

“…The major advantage of rhabdoviruses is that they are able to replicate in both plants and insect vectors, providing a convenient and highly efficient approach for transmission of gene editing reagents to multiple hosts through virus infection and insect transmission. Previous studies of MMV transmission biology, molecular interactions between P. maidis and the virus, and genome editing in the vector make this system a good platform for further characterizing rhabdovirus-vector interactions (Barandoc-Alviar et al, 2016; Barandoc-Alviar et al, 2022; Klobasa et al, 2021). Other rhabdoviruses, BYSMV (Gao et al, 2019) and SYNV (Ma et al, 2020), can express multiple guide RNAs and Cas nucleases.…”

In silico analysis of the predicted protein-protein interaction of syntaxin-18, a putative receptor of Peregrinus maidis Ashmead (Hemiptera: Delphacidae) with Maize mosaic virus glycoprotein

“…It is a known vector of two disease-causing plant pathogens of corn, the Maize mosaic virus (MMV) and the Maize stripe virus (MStV) (Jourdan-Ruf et al, 1995). Once P. maidis acquires MMV, the virus persistsand replicates in the insect reaching a threshold level in the adult stage (Barandoc-Alviar et al, 2017). MMV is the causal agent of the mosaic disease of corn broadly occurring in Africa, Asia, and in the Americas (Centre for Agriculture and Bioscience International (CABI).…”