Identification of interior salt-tolerant bacteria from ice plant Mesembryanthemum crystallinum and evaluation of their promoting effects

Jian, Zhang; Wang, Pengcheng; Tian, Hongmei; Jiang, Haikun; Wang, Yan; Yan, Chao‐Guo

doi:10.1007/s13199-018-0551-6

Cited by 11 publications

(17 citation statements)

References 54 publications

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“…Kaixuan and the substrate for culturing the tomato seedlings were the same as mentioned above. The tomato seeds was sterilized by washing three times with sterile water, soaking in 5% (v/v) sodium hypochlorite solution for 5 min, and soaking in 70% (v/v) ethanol for 5 min then washing 10 times with sterile water 2 8 . The Streptomyces sp .…”

Section: Methodsmentioning

confidence: 99%

Streptomyces sp. strain TOR3209: a rhizosphere bacterium promoting growth of tomato by affecting the rhizosphere microbial community

et al. 2020

Sci Rep

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Aiming at revealing the possible mechanism of its growth promoting effect on tomato, the correlations among Streptomyces sp. TOR3209 inoculation, rhizobacteriome, and tomato growth/production traits were investigated in this study. By analyses of Illumina sequencing and plate coating, differences in rhizosphere microbial communities were found in different growth stages and distinct inoculation treatments. The plant biomass/fruit yields and relative abundances of families Flavobacteriaceae, Sphingobacteriaceae, Polyangiaceae and Enterobacteriaceae in treatments T (tomato inoculated with TOR3209) and TF (tomato inoculated with TOR3209 + organic fertilizer) were higher than that in the controls (CK and CK+ organic fertilizer), respectively. The analysis of Metastats and LEfSe revealed that the genera Flavobacterium and Sorangium in seedling stage, Klebsiella in flowering stage, Collimonas in early fruit setting stage, and genera Micrococcaceae, Pontibacte and Adhaeribacter in late fruit setting stage were the most representative rhizobacteria that positively responded to TOR3209 inoculation. By cultivation method, five bacterial strains positively correlated to TOR3209 inoculation were isolated from rhizosphere and root endosphere, which were identified as tomato growth promoters affiliated to Enterobacter sp., Arthrobacter sp., Bacillus subtilis, Rhizobium sp. and Bacillus velezensis. In pot experiment, TOR3209 and B. velezensis WSW007 showed joint promotion to tomato production, while the abundance of inoculated TOR3209 was dramatically decreased in rhizosphere along the growth of tomato. Conclusively, TOR3209 might promote the tomato production via changing of microbial community in rhizosphere. These findings provide a better understanding of the interactions among PGPR in plant promotion.

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Section: Methodsmentioning

confidence: 99%

Streptomyces sp. strain TOR3209: a rhizosphere bacterium promoting growth of tomato by affecting the rhizosphere microbial community

et al. 2020

Sci Rep

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“…strain MC1 (GenBank accession number: MF431770; Transcriptome number: SRP235179) was isolated from the interior stem of ice plants and verified as a bacterial endophyte. The strain shows plant growth-promoting effects and improved growth of cabbage seedlings under salt stress [26]. MC1 was cultivated on nutrient solid agar plates (5.0 g/L yeast extract, 10.0 g/L peptone, and 15.0 g agar at pH 6.9-7.1) containing 1.71 M NaCl at 25 • C ± 2 • C for five days.…”

Section: Strain and Culture Conditionmentioning

confidence: 99%

“…The salt-resistance activity of MC1 was determined in an activated bacterial culture grown in solid medium with (1.71 M NaCl) or without (CK, 0 M NaCl) salt. The concentration of NaCl was determined based on the optimal growth condition of strain MC1 [26]. Bacterial concentration was adjusted to 10 8 CFU/mL.…”

Section: Induction Of Salt Tolerance and Bacterial Collectionmentioning

confidence: 99%

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Transcriptome Analysis of Ice Plant Growth-Promoting Endophytic Bacterium Halomonas sp. Strain MC1 to Identify the Genes Involved in Salt Tolerance

et al. 2020

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Salt stress is an important adverse condition encountered during plant and microbe growth in terrestrial soil ecosystems. Currently, how ice plant (Mesembryanthemum crystallinum) growth-promoting endophytic bacteria (EB) cope with salt stress and regulate growth and the genes responsible for salt tolerance remain unknown. We applied RNA-Seq technology to determine the growth mechanism of the EB Halomonas sp. MC1 strain and the genes involved in salt tolerance. A total of 893 genes were significantly regulated after salt treatment. These genes included 401 upregulated and 492 downregulated genes. Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes analysis revealed that the most enriched genes included those related to the outer membrane-bounded periplasmic space, ATPase activity, catabolic process, and proton transmembrane transport. The quantitative real-time polymerase chain reaction data were similar to those obtained from RNA-Seq. The MC1 strain maintained survival under salt stress by regulating cellular and metabolic processes and pyruvate metabolism pathways such as organic and carboxylic acid catabolic pathways. We highlighted the response mechanism of Halomonas sp. MC1 to fully understand the dynamics of complex salt–microbe interactions.

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“…M. crystallinum L. (2n = 18) ( Figure 1) is an annual succulent belonging to the Aizoaceae family and is native to South and East Africa, the Sinai, and southern Europe (Spain) [4,14,15]. It is also naturalized in Mediterranean coastal areas, the U.S., Australia, Mexico, Chile, and the Caribbean [16][17][18][19].…”

Section: Origin and Diffusionmentioning

confidence: 99%

Halophyte Common Ice Plants: A Future Solution to Arable Land Salinization

Loconsole

Murillo-Amador²,

Cristiano

et al. 2019

Sustainability

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The problems associated with the salinization of soils and water bodies and the increasing competition for scarce freshwater resources are increasing. Current attempts to adapt to these conditions through sustainable agriculture involves searching for new highly salt-tolerant crops, and wild species that have potential as saline crops are particularly suitable. The common ice plant (Mesembryanthemum crystallinum L.) is an edible halophyte member of the Aizoaceae family, which switches from C3 photosynthesis to crassulacean acid metabolism (CAM) when exposed to salinity or water stress. The aim of this review was to examine the potential of using the ice plant in both the wild and as a crop, and to describe its ecology and morphology, environmental and agronomic requirements, and physiology. The antioxidant properties and mineral composition of the ice plant are also beneficial to human health and have been extensively examined.

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Identification of interior salt-tolerant bacteria from ice plant Mesembryanthemum crystallinum and evaluation of their promoting effects

Cited by 11 publications

References 54 publications

Streptomyces sp. strain TOR3209: a rhizosphere bacterium promoting growth of tomato by affecting the rhizosphere microbial community

Streptomyces sp. strain TOR3209: a rhizosphere bacterium promoting growth of tomato by affecting the rhizosphere microbial community

Transcriptome Analysis of Ice Plant Growth-Promoting Endophytic Bacterium Halomonas sp. Strain MC1 to Identify the Genes Involved in Salt Tolerance

Halophyte Common Ice Plants: A Future Solution to Arable Land Salinization

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