Identification of Japanese Flounder Leucocytes Involved in the Host Response to Neoheterobothrium hirame.

Nakayasu, Chihaya; Tsutsumi, Nobuyuki; Yoshitomi, Tomoyasu; Yoshinaga, Tomoyoshi; Kumagai, Akira

doi:10.3147/jsfp.38.9

Cited by 8 publications

(15 citation statements)

References 23 publications

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“…The present results, together with the previous observations on the leucocyte types involved in the host response to Neoheterobothrium hirame (Nakayasu et al 2003), show that infiltrating cells mainly consists of granulocytes, macrophages and DGCs. After the appearance of mature parasites, lymphocytes, thrombocytes and erythrocytes decreased rapidly in the blood as the anemia progressed.…”

Section: Discussionsupporting

confidence: 87%

“…These slides were stained with May-Grünwald and Giemsa, and observed under a light microscope. The ratios of lymphocytes, granulocytes, and monocytes in PBLs were determined using these slides as described by Nakayasu et al (2003). The flounder has only 1 type of granulocyte in the blood (Nakayasu et al 2003).…”

Section: Methodsmentioning

confidence: 99%

“…The ratios of lymphocytes, granulocytes, and monocytes in PBLs were determined using these slides as described by Nakayasu et al (2003). The flounder has only 1 type of granulocyte in the blood (Nakayasu et al 2003). Moreover, since sphericalshaped thrombocytes are difficult to distinguish from lymphocytes, these cells were counted as lymphocytes.…”

Section: Methodsmentioning

confidence: 99%

“…Electron microscopy showed the presence of leucocytes directly adhered to the parasite tegument. Identification of infiltrated and adhered cells was based on the morphological criteria described by Nakayasu et al (2003 were also observed in the infected sites. On Day 47, parasite clamps were observed in muscle and connective tissue where marked infiltration of inflammatory cells and fibroblasts had occurred (Fig.…”

Section: Sequential Histological Change In Infected Tissue Withmentioning

confidence: 99%

“…In the host tissue surrounding the parasite, intensive infiltration of inflammatory cells and necrosis of host cells were observed (Anshary & Ogawa 2001). Nakayasu et al (2003) also reported that the infiltrated cells, which consisted of macrophages, granulocytes and the cells with highly electron-dense granules (DGC), adhered to the tegument of Neoheterobothrium hirame. These infiltrated or adhered cells are likely to play important roles in the elimination of N. hirame in the flounder.…”

Section: Introductionmentioning

confidence: 99%

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Role of cellular response in elimination of the monogenean Neoheterobothrium hirame in Japanese flounder Paralichthys olivaceus

Nakayasu¹,

Tsutsumi²,

Oseko³

et al. 2005

Dis. Aquat. Org.

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Adult worms of the blood-feeding monogenean parasite Neoheterobothrium hirame, which cause anemia in the Japanese flounder Paralichthys olivaceus, attach to the host fish by embedding their posterior part deeply into the host tissue. To investigate the possibility that cellular responses of the host fish can eliminate N. hirame, flounder were experimentally infected with N. hirame larvae and reared in either fed or starved conditions. Mature parasites were identified on the buccal cavity wall of the fish 33 d post-infection (Day 33). Monocytes/macrophages and granulocytes increased rapidly in the blood and infected sites after the appearance of mature parasites. These cells adhered to the tegument of the parasites. In addition, a few cells with large electron-dense granules (DGCs) were observed in the inflammatory foci. On Day 47, the tegument of some parasites collapsed partially and were phagocytosed by the infiltrated host cells. Some infiltrated cells adhered directly to the inner tissues of the parasites. On Day 54, in the fed fish group, the loss of the tegument led to damage of the parasites' inner tissue by a large number of infiltrated cells. In this group, the elimination of the parasites was noted from Day 47 to 54. These observations probably suggest that the cellular response of the host fish destructed the parasite's posterior part embedded in the tissue, thereby eliminating the parasites. On the other hand, a high mortality was observed in the starved group. The starved fish developed much more severe anemia than the fed fish, and the elimination of the parasites was not observed in this group. The results of the present study suggest that flounder can eliminate N. hirame if they are fed sufficiently. KEY WORDS: Flounder · Leucocytes response · Neoheterobothrium hirame · Monogenean parasiteResale or republication not permitted without written consent of the publisher

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Section: Discussionsupporting

confidence: 87%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Sequential Histological Change In Infected Tissue Withmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Role of cellular response in elimination of the monogenean Neoheterobothrium hirame in Japanese flounder Paralichthys olivaceus

Nakayasu¹,

Tsutsumi²,

Oseko³

et al. 2005

Dis. Aquat. Org.

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Production and characterization of monoclonal antibodies to leukocytes and serum immunoglobulin in Japanese flounder Paralichthys olivaceus

et al. 2009

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Two monoclonal antibodies (MAbs: JFW1 and JFW10) were produced against peripheral blood leukocytes (PBL) in Japanese flounder. Additionally, MAbs against flounder immunoglobulin (Ig; JFW20 and JFW21) were generated for the surface marker of Ig ? leukocytes using purified serum Ig as an antigen. MAb JFW1 recognized the surface marker of granulocytes and monocytes and MAb JFW10 specifically bound to the surface antigen of thrombocytes. Flow cytometric analysis of PBL incubated with JFW1, JFW10, JFW20 and JFW21 revealed that 2.5-7.4, 23.7-50.1, 25.2-26.1 and 5.2-8.3% of all leukocytes were positive for these markers. Analysis of head kidney leukocytes (HKL) showed that JFW1, JFW10, JFW20 and JFW21 bound to 30.5-36.3, 1.9-2.8, 6.4-8.3 and 1.9-3.0% of all leukocytes, respectively. Western blot analysis after SDS-PAGE showed that JFW10 recognizes a protein of 115 kDa from lysed PBL. JFW20 recognized the 70 and 74 kDa proteins of the heavy chain of Ig from serum. No band was observed for either JFW1 or JFW21. These antibodies will be useful for the identification and isolation of Japanese flounder leukocyte subpopulations and will facilitate immunological studies of flounder.

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Gene expression of leucocytes in vaccinated Japanese flounder (Paralichthys olivaceus) during the course of experimental infection with Edwardsiella tarda

Matsuyama

Fujiwara

Nakayasu

et al. 2007

Fish & Shellfish Immunology

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Identification of Japanese Flounder Leucocytes Involved in the Host Response to Neoheterobothrium hirame.

Cited by 8 publications

References 23 publications

Role of cellular response in elimination of the monogenean Neoheterobothrium hirame in Japanese flounder Paralichthys olivaceus

Role of cellular response in elimination of the monogenean Neoheterobothrium hirame in Japanese flounder Paralichthys olivaceus

Production and characterization of monoclonal antibodies to leukocytes and serum immunoglobulin in Japanese flounder Paralichthys olivaceus

Gene expression of leucocytes in vaccinated Japanese flounder (Paralichthys olivaceus) during the course of experimental infection with Edwardsiella tarda

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