Identification of Key Functions Required for Production and Utilization of the Siderophore Piscibactin Encoded by the High-Pathogenicity Island irp-HPI in Vibrionaceae

Lages, Marta A; Ageitos, Lucía; Rodríguez, Jaime; Jiménez, Carlos; Lemos, Manuel L.; Balado, Miguel

doi:10.3390/ijms23168865

Cited by 3 publications

(2 citation statements)

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“…The previously constructed plasmids pMB276, pMB277, and pML212 ( 16 , 25 ) carrying the lacZ fusions P frpA::lacZ , P pbtA::lacZ, and P frpC::lacZ ( Fig. 1 ) into plasmid pPHRP309 ( 33 ) were mobilized from E. coli S17-1 λpir ( 34 ) to V. anguillarum RV22 and the derivative mutants Δ pbtA and Δ pbtA N by conjugation.…”

Section: Methodsmentioning

confidence: 99%

“…The irp -HPI encodes an AraC-like transcriptional regulator named PbtA ( Fig. 1 ), whose inactivation disables the expression of piscibactin biosynthesis and transport genes ( 24 , 25 ). PbtA expression is greatly induced with temperature decrease, making it the main activator of the piscibactin siderophore system expression under low temperature.…”

Section: Introductionmentioning

confidence: 99%

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Remodulation of bacterial transcriptome after acquisition of foreign DNA: the case of irp -HPI high-pathogenicity island in Vibrio anguillarum

Lages,

do Vale,

Lemos

et al. 2024

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The high-pathogenicity island irp -HPI is widespread in Vibrionaceae and encodes the siderophore piscibactin, as well as the regulator PbtA that is essential for its expression. In this work, we aim to study whether PbtA directly interacts with irp -HPI promoters. Furthermore, we hypothesize that PbtA, and thereby the acquisition of irp -HPI island, may also influence the expression of other genes elsewhere in the bacterial genome. To address this question, an RNAseq analysis was conducted to identify differentially expressed genes after pbtA deletion in Vibrio anguillarum RV22 genetic background. The results showed that PbtA not only modulates the irp -HPI genes but also modulates the expression of a plethora of V. anguillarum core genome genes, inducing nitrate, arginine, and sulfate metabolism, T6SS1, and quorum sensing, while repressing lipopolysaccharide (LPS) production, MARTX toxin, and major porins such as OmpV and ChiP. The direct binding of the C-terminal domain of PbtA to piscibactin promoters (P frpA and P frpC ), quorum sensing ( vanT ), LPS transporter wza, and T6SS structure- and effector-encoding genes was demonstrated by electrophoretic mobility shift assay (EMSA). The results provide valuable insights into the regulatory mechanisms underlying the expression of irp -HPI island and its impact on Vibrios transcriptome, with implications in pathogenesis. IMPORTANCE Horizontal gene transfer enables bacteria to acquire traits, such as virulence factors, thereby increasing the risk of the emergence of new pathogens. irp -HPI genomic island has a broad dissemination in Vibrionaceae and is present in numerous potentially pathogenic marine bacteria, some of which can infect humans. Previous works showed that certain V. anguillarum strains exhibit an expanded host range plasticity and heightened virulence, a phenomenon linked to the acquisition of the irp -HPI genomic island. The present work shows that this adaptive capability is likely achieved through comprehensive changes in the transcriptome of the bacteria and that these changes are mediated by the master regulator PbtA encoded within the irp -HPI element. Our results shed light on the broad implications of horizontal gene transfer in bacterial evolution, showing that the acquired DNA can directly mediate changes in the expression of the core genome, with profounds implications in pathogenesis.

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