Identification of key gene networks controlling anthocyanin biosynthesis in peach flower

Khan, Irshad Ahmad; Cao, Ke; Guo, Jian; Li, Yong; Wang, Qi; Yang, Xin; Wu, Jicheng; Fang, Weichao; Wang, Lirong

doi:10.1016/j.plantsci.2021.111151

Cited by 31 publications

(20 citation statements)

References 50 publications

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“…PyMYB114 (isolated from Chinese pear) and PpMYB114 (isolated from 'Red Zaosu' pear) were found to interact with other transcription factors to co-regulate fruit anthocyanin biosynthesis in pear (Yao et al, 2017;Ni et al, 2019). Overexpression of PpMYB108 (isolated from peach flower) was confirmed to significantly increased anthocyanin biosynthesis in tobacco flowers by a positive interaction with PpDFR promoter (Khan et al, 2022). OsC1 (isolated from rice leaves) was verified to activate all anthocyanin biosynthetic genes including OsCHS, OsCHI, OsF3'H, OsF3H, OsDFR and OsANS in rice leaves by forming MYB-bHLH-WD40 complex with OsRb and OsPAC1 (Zheng et al, 2019).…”

Section: Key Candidate Tfs Involved In Anthocyanin Synthesis In R Rug...mentioning

confidence: 94%

“…Few MYB TFs regulating anthocyanin synthesis were identified from Rosaceae ornamental plants, such as RcMYB114 in Rosa sp. and PpMYB108 in peach flower (Li et al, 2022;Khan et al, 2022).…”

Section: Key Candidate Tfs Involved In Anthocyanin Synthesis In R Rug...mentioning

confidence: 99%

“…By binding to the promoter regions of structural genes, some transcription factors (TFs) are studied the effect on the synthesis of anthocyanins, such as MYB, bHLH, WD, bZIP, and MADSbox (An et al, 2017;Lu et al, 2018;Jian et al, 2019;Khan et al, 2022). Among them, the regulatory function of MYB, bHLH, and WD40 families are well stablished, which can play regulatory roles alone or by consisting the MBW (MYB-bHLH-WD) protein complex (Ramsay and Glover, 2005;Feng et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome and chemical analyses revealed the mechanism of flower color formation in Rosa rugosa

Wang

Li²,

Zhu³

et al. 2022

Front. Plant Sci.

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Rosa rugosa is a famous Chinese traditional flower with high ornamental value and well environmental adapt ability. The cultivation of new colorful germplasms to improve monotonous flower color could promote its landscape application. However, the mechanism of flower color formation in R. rugosa remains unclear. In this study, combined analyses of the chemical and transcriptome were performed in the R. rugosa germplasms with representative flower colors. Among the identified anthocyanins, cyanidin 3,5-O-diglucoside (Cy3G5G) and peonidin 3,5-O-diglucoside (Pn3G5G) were the two dominant anthocyanins in the petals of R. rugosa. The sum content of Cy3G5G and Pn3G5G was responsible for the petal color intensity, such as pink or purple, light- or dark- red. The ratio of Cy3G5G to Pn3G5G was contributed to the petal color hue, that is, red or pink/purple. Maintaining both high relative and high absolute content of Cy3G5G may be the precondition for forming red-colored petals in R. rugosa. Cyanidin biosynthesis shunt was the dominant pathway for anthocyanin accumulation in R. rugosa, which may be the key reason for the presence of monotonous petal color in R. rugosa, mainly pink/purple. In the upstream pathway of cyanidin biosynthesis, 35 differentially expressed structural genes encoding 12 enzymes co-expressed to regulate the sum contents of Cy3G5G and Pn3G5G, and then determined the color intensity of petals. RrAOMT, involved in the downstream pathway of cyanidin biosynthesis, regulated the ratio of Cy3G5G to Pn3G5G via methylation and then determined the color hue of petals. It was worth mentioning that significantly higher delphinidin-3,5-O-diglucoside content and RrF3’5’H expression were detected from deep purple-red-flowered 8-16 germplasm with somewhat unique and visible blue hue. Three candidate key transcription factors identified by correlation analysis, RrMYB108, RrC1, and RrMYB114, might play critical roles in the control of petal color by regulating the expression of both RrAOMT and other multiple structural genes. These results provided novel insights into anthocyanin accumulation and flower coloration mechanism in R. rugosa, and the candidate key genes involved in anthocyanin biosynthesis could be valuable resources for the breeding of ornamental plants in future.

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Section: Key Candidate Tfs Involved In Anthocyanin Synthesis In R Rug...mentioning

confidence: 94%

“…Few MYB TFs regulating anthocyanin synthesis were identified from Rosaceae ornamental plants, such as RcMYB114 in Rosa sp. and PpMYB108 in peach flower (Li et al, 2022;Khan et al, 2022).…”

Section: Key Candidate Tfs Involved In Anthocyanin Synthesis In R Rug...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Transcriptome and chemical analyses revealed the mechanism of flower color formation in Rosa rugosa

Wang

Li²,

Zhu³

et al. 2022

Front. Plant Sci.

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“…Two types of genetic regulation play a role in the synthesis of anthocyanin in plants. Among them, the first is the biosynthetic genes, which encode the enzymes required for anthocyanin syntheses (Feng et al 2016;Khan et al 2022), including chalcone synthase (CHS), chalcone isomerase (CHI), dihydroflavonol-4-reductase (DFR), leucoanthocyanidin dioxygenase (LDOX), and UDP-glycose (Khan et al 2022), and the second are regulatory genes that regulate the magnitude and structure of the biosynthetic genes (Feng et al 2016). With this type, anthocyanin regulation studies focus on R2R3-myeloblastosis (MYB), a basic helix-loop-helix (bHLH), and WD40 groups (Feng et al 2013).…”

Section: Role Of Bhlh Family Genes In Anthocyanin Biosynthesismentioning

confidence: 99%

Diverse role of basic Helix-Loop-Helix (bHLH) transcription factor superfamily genes in the fleshy fruit-bearing plant species

Noor¹,

Uddin²,

Khan³

et al. 2022

CZECH J GENET PLANT

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The basic Helix-Loop-Helix (bHLH) superfamily is the most widespread family of transcription factors in eukaryotic organisms, which can activate the expression of genes by interacting with specific promoters in the genes. The bHLH transcription factors direct the development and metabolic process of plants, including flowering initiation and secondary metabolite production, by attaching to specific sites on their promoters. These transcription factors are essential for encouraging plant tolerance or the adjustment to harsh environmental conditions. The involvement of bHLH genes in anthocyanin formation in fleshy fruit-bearing plants, as well as the role of these genes in response to stimuli including drought, salt, and cold stress, are discussed in this article. New concepts and goals for the production of stress-tolerant fruit species are suggested. Furthermore, solid evidence for the critical role of bHLH genes in the growth and development, as well as anthocyanin biosynthesis in fleshy fruit plants, are also presented in this article. This review identifies several future research directions that can shed light on the roles of bHLH genes in fruit-bearing plants and will assist the use of these genes in efforts to breed fruit crop varieties that are more resistant to stress. Generally, there has been little research carried out on the role of bHLHs transcription factor family genes in fleshy fruit-bearing plant species and more in-depth studies are required to fully understand the diverse role of bHLH genes in these species.

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“…The structural genes directly encode an assemblage of enzymes, including phenylalanine ammonia lyase (PAL), chalcone synthase (CHS), chalcone isomerase (CHI), flavonoid 3‐hydroxylase (F3H), dihydroflavonol reductase (DFR), anthocyanin synthase (ANS), and anthocyanin 3‐O‐glucosyltransferase (UFGT) (Hara et al, 2003 ; Li et al, 2014 ). The genes for these enzymes can be regulated by the transcription of regulatory genes, such as WRKY, bHLH (Arlotta et al, 2020 ), MYB (Khan et al, 2022 ; Li et al, 2020 ), WD40 (Wang et al, 2020 ), and HY5 (Bustamante et al, 2018 ). In addition, some regulatory genes have also been reported to be related to the biosynthesis of anthocyanin, including squamosa promoter‐binding protein‐like (SPL) (Yang et al, 2021 ), auxin response factor (ARF) (Wang et al, 2020 ), and Jasmonate Zim‐domain (JAZ) (Adrian et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Identification of microRNAs and their target genes related to the accumulation of anthocyanin in purple potato tubers (Solanum tuberosum)

et al. 2022

Plant Direct

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MicroRNAs (miRNAs) are types of endogenous non‐coding small RNAs found in eukaryotes that are 18–25 nucleotides long. miRNAs are considered to be key regulatory factors of the expression of target mRNA. The roles of miRNAs involved in the regulation of anthocyanin accumulation in pigmented potatoes have not been systematically reported. In this study, the differentially expressed miRNAs and their target genes involved in the accumulation of anthocyanin during different developmental stages in purple potato ( Solanum tuberosum L.) were identified using small RNA (sRNA) and degradome sequencing. A total of 275 differentially expressed miRNAs were identified in the sRNA libraries. A total of 69,387,200 raw reads were obtained from three degradome libraries. The anthocyanin responsive miRNA–mRNA modules were analyzed, and 37 miRNAs and 23 target genes were obtained. Different miRNAs regulate the key enzymes of anthocyanin synthesis in purple potato. The structural genes included phenylalanine ammonia lyase, chalcone isomerase, flavanone 3‐hydroxylase, and anthocyanidin 3‐ O ‐glucosyltransferase. The regulatory genes included WD40, MYB, and SPL9. stu‐miR172e‐5p_L‐1R‐1, stu‐miR828a, stu‐miR29b‐4‐5p, stu‐miR8019‐5p_L‐4R‐3, stu‐miR396b‐5p, stu‐miR5303f_L‐7R + 2, stu‐miR7997a_L‐3, stu‐miR7997b_L‐3, stu‐miR7997c_L + 3R‐5_2ss2TA3AG, stu‐miR156f‐5p_L + 1, stu‐miR156a, stu‐miR156a_R‐1, stu‐miR156e, stu‐miR858, stu‐miR5021, stu‐miR828 and their target genes were validated by qRT‐PCR. They play important roles in the coloration and accumulation of purple potatoes. These results provide new insights into the biosynthesis of anthocyanins in pigmented potatoes.

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Identification of key gene networks controlling anthocyanin biosynthesis in peach flower

Cited by 31 publications

References 50 publications

Transcriptome and chemical analyses revealed the mechanism of flower color formation in Rosa rugosa

Transcriptome and chemical analyses revealed the mechanism of flower color formation in Rosa rugosa

Diverse role of basic Helix-Loop-Helix (bHLH) transcription factor superfamily genes in the fleshy fruit-bearing plant species

Identification of microRNAs and their target genes related to the accumulation of anthocyanin in purple potato tubers (Solanum tuberosum)

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