Identification of key genes and immune infiltration modulated by CPAP in obstructive sleep apnea by integrated bioinformatics analysis

Abstract

Patients with obstructive sleep apnea (OSA) experience partial or complete upper airway collapses during sleep resulting in nocturnal hypoxia-normoxia cycling, and continuous positive airway pressure (CPAP) is the golden treatment for OSA. Nevertheless, the exact mechanisms of action, especially the transcriptome effect of CPAP on OSA patients, remain elusive. The goal of this study was to evaluate the longitudinal alterations in peripheral blood mononuclear cells transcriptome profiles of OSA patients in order to identify the hub gene and immune response. GSE133601 was downloaded from Gene Expression Omnibus (GEO). We identified black module via weighted gene co-expression network analysis (WGCNA), the genes in which were correlated significantly with the clinical trait of CPAP treatment. Finally, eleven hub genes (TRAV10, SNORA36A, RPL10, OBP2B, IGLV1-40, H2BC8, ESAM, DNASE1L3, CD22, ANK3, ACP3) were traced and used to construct a random forest model to predict therapeutic efficacy of CPAP in OSA with a good performance with AUC of 0.92. We further studied the immune cells infiltration in OSA patients with CIBERSORT, and monocytes were found to be related with the remission of OSA and partially correlated with the hub genes identified. In conclusion, these key genes and immune infiltration may be of great importance in the remission of OSA and related research of these genes may provide a new therapeutic target for OSA in the future.

Construction of a mitochondrial dysfunction related signature of diagnosed model to obstructive sleep apnea

“…Our data demonstrated the differences in infiltrating immune cells between OSA and control samples, and these cells may also be responsible for OSA comorbidities. Immune cell infiltration may also be of great importance in the remission of OSA ( Fan et al, 2021 ). Consistent with our findings, CIH in OSA was previously found to induce adipose tissue macrophages towards a pro-inflammatory M1 subtype ( Ryan 2017 ), and macrophages are known to contribute to adipose tissue insulin resistance and vascular atherogenesis ( Trzepizur et al, 2018 ).…”

Dysregulation of immune response in PCOS organ system

“…OSA is characterized by repeated partial or complete airway collapse that may lead to intermittent hypoxia. Intermittent hypoxia further contributes to oxidative imbalance, producing reactive oxygen species, numerous cytokines such as IL-2, IL-4, IL-6, lipid peroxidation, and free DNA (153). There has long been much evidence that women with PCOS have a higher prevalence of OSA than the normal population (154)(155)(156).…”