Identification of lactic acid bacteria isolated from corn stovers

Pang, Huili; Zhang, Meng; Guangyong, Qin; Tan, Zhongfang; Li, Zongwei; Wang, Yanping; Cai, Yimin

doi:10.1111/j.1740-0929.2011.00894.x

Cited by 47 publications

(40 citation statements)

References 30 publications

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“…This finding is in agreement with Pang et al . () who found carbohydrate fermentation patterns showed ambiguity. All strains were inhibited at 45°C except AZZ4 and AZZ7, while they were able to grow at pH range of 4·0–8·0.…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic identification of lactic acid bacteria isolates and their effects on the fermentation quality of sweet sorghum (Sorghum bicolor) silage

Sifeeldein

Wang

et al. 2019

J Appl Microbiol

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Aim To isolate, screen and identify the lactic acid bacteria (LAB) from elephant silage during the process of the fermentation and their effects on the fermentation quality of sweet sorghum silage. Methods and Results The isolated strains were identified based on morphological, physiological and biochemical characteristics as well as 16S rRNA analysis. Seven LAB strains were isolated from elephant grass silage. Three strains (Pediococcus acidilactici (AZZ1), Lactobacillus plantarum subsp. plantarum (AZZ4), L. plantarum subsp. argentoratensis (AZZ7) and one commercial bacteria L. plantarum, ecosyl MTD/1(CB)) were chosen as additives at 6 log colony forming units per gram of fresh sweet sorghum grass in laboratory silos (680 g). Silos for each treatment were opened after 5, 7, 14, 30 and 60 days, respectively. All isolates were characterized as Gram‐positive, catalase‐negative and grow normally in 6·5% NaCl. The strains AZZ1, AZZ2 and AZZ5, were identified as Pediococcus genus while AZZ3, AZZ4, AZZ6 and AZZ7 were Lactobacillus genus. Compared to the control, all the isolates improved the silage quality of sweet sorghum silage, indicated by significantly (P < 0·05) lower pH and ammonia‐nitrogen contents and undesirable micro‐organism counts, and higher lactic acid (LA) contents and ratios of lactic acid/acetic acid. During ensiling, AZZ4 performed better among all of inoculants, indicated by significantly (P < 0·05) decreased on pH and ammonia‐N contents and higher increased on LA contents. Conclusion Strain AZZ4 is recommended as starter culture for sweet sorghum. Significance and Impact of the Study This is the first time to investigate the effects of LAB isolates from elephant grass silage and use them as additives, which is to find out how LAB inoculants improve the fermentation quality of sweet sorghum silage.

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Section: Discussionmentioning

confidence: 99%

Phylogenetic identification of lactic acid bacteria isolates and their effects on the fermentation quality of sweet sorghum (Sorghum bicolor) silage

Sifeeldein

Wang

et al. 2019

J Appl Microbiol

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“…Yang et al (2010) found that the number of LAB on three vegetables and other plant residue to be 10 3 -10 4 cfu g À1 , with Lactobacillus being the predominant taxa. Pang et al (2011) reported that LAB in corn stover were predominantly Lactobacillus (85.6%), consisted of L. plantarum (33.3%), L. pentosus (28.6%) and L. brevis (23.7%), and Leu. Lactobacillus accounted for 40.00%, 41.67%, 83.33% and 16.67%, while Lactococcus accounted for 60.00%, 8.33%, 16.67% and 83.33%, on MT-1, Mott, Reyan and Guimu, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…It is noteworthy that we found that the WSC content of Pennisetum grass was positively related to the abundance of LAB on Pennisetum grass, suggesting that this resources a driver of LAB populations. It is noteworthy that the number of epiphytic LAB that develop on corn, which has a high WSC content, is much higher (10 6 -10 7 cfu g À1 ) (Pang et al 2011) than that on plants such as guinea grass or naked barley straw (10 2 -10 4 cfu g À1 ), which have much lower WSC content (Zhang et al 1997. Mercier and Lindow (2000) reported that simple sugars, such as glucose, fructose and sucrose which were the main substrate for LAB fermentation, were the dominant carbon sources on the plants and were thought to simply leach from the interior of the plant.…”

Section: Discussionmentioning

confidence: 99%

Factors influencing the distribution of lactic acid bacteria on Pennisetum grasses

et al. 2017

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Successful silage production depends on the promotion of lactic acid fermentation and one of the key factors influencing the fermentation quality of silage is lactic acid bacteria (LAB). Therefore, epiphytic LAB on grasses play an important role in silage production. The present research investigated the distribution of LAB on Pennisetum spp. and factors influencing its presence. Lactic acid bacteria on four grass cultivars, cv. MT‐1, cv. Mott, cv. Reyan No. 4 (Reyan) and cv. Guimu No. 1 (Guimu), were investigated by both culture‐dependent methods and 16S rRNA amplicon sequence analysis and polymerase chain reaction‐denaturing gradient gel electrophoresis (PCR‐DGGE). Mott grass had significantly lower contents of dry matter and lower pH and higher water‐soluble carbohydrates (WSC) content than the other three cultivars (P < 0.05). Mott also epiphyted the largest number of LAB (2.56 × 103 cfu g−1), while MT‐1 supported the least (41 cfu g−1). A diversity of LAB were recovered from the species including the following proportional representation: 33.4% Lactobacillus plantarum, 8.3% Lactobacillus pentosus, 8.3% Lactococcus lactis, 8.3% Leuconostoc citreum, 25.0% Weissella confusa and 16.7% Weissella cibaria on Mott, 20.0% L. plantarum, 20.0% L. pentosus and 60.0% Lc. lactis on MT‐1, 83.3% L. plantarum and 16.7% Lc. lactis on Reyan, 16.7% L. plantarum and 83.3% Lc. lactis on Guimu. The DGGE analyses of rRNA amplicons also revealed a similar distribution of these species. While the species composition and abundance of LAB differed among the four grasses. The number of LAB on grasses was positively correlated with the WSC content in the grasses (P < 0.01), and negatively correlated with the pH of the grasses (P < 0.05).

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“…and an uncultured bacterium. Pang et al (2011b) identified strains isolated from maize stover, and 86% of the LAB consisted of L. plantarum, Lactobacillus pentosus and L. brevis, three species commonly reported in silages. On the other hand they reported the presence of Leuconostoc lactis, Enterococcus mundtii and Weissella cibaria, the latter a recently described species.…”

Section: New Microbial Species In Silagementioning

confidence: 99%

Recent advances in silage microbiology

Muck

2013

AFSci

179

123

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Recent advances in silage microbiology are reviewed. Most new techniques in silage microbiology use the polymerase chain reaction (PCR) to make copies of a portion of the DNA in microorganisms. These techniques allow us to identify and quantify species as well as do community analysis. The PCR-based techniques are uncovering new species, both bacteria and fungi, during storage and feeding. Silage inoculants are widely available, but of greater interest has been research investigating why inoculants are so successful. Various inoculant strains have been found to produce bacteriocins and other compounds that inhibit other bacteria and fungi, improving their chances for success. In vitro ruminal fermentation research is showing that some inoculated silages affect rumen microorganisms, reducing methane in some cases and increasing microbial biomass production in others. Better understanding of silage microbiology will allow us to better manage silos and develop better inoculants to improve silage quality.

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Identification of lactic acid bacteria isolated from corn stovers

Cited by 47 publications

References 30 publications

Phylogenetic identification of lactic acid bacteria isolates and their effects on the fermentation quality of sweet sorghum (Sorghum bicolor) silage

Phylogenetic identification of lactic acid bacteria isolates and their effects on the fermentation quality of sweet sorghum (Sorghum bicolor) silage

Factors influencing the distribution of lactic acid bacteria on Pennisetum grasses

Recent advances in silage microbiology

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