Identification of lipidomic responses to GCRV infection in Ctenopharyngodon idellus kidney (CIK) cells

Zhang, Xing; Huang, Jingcai; Shen, Zeen; Wang, Yan; Wang, Yujun; Gong, Chengliang; Hu, Xiaolong; Wang, Chonglong

doi:10.1016/j.aquaculture.2020.736106

Cited by 4 publications

(1 citation statement)

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“…Notably, GCRV-II displayed an additional abundance of differentially expressed genes involved in lipid biosynthesis and metabolic processes [49]. Ultrastructural analysis of GCRV-II-infected CIK cells revealed the presence of an extra lipid membrane surrounding viral particles [50]. Since GCRV is a non-enveloped virus, we speculate that this lipid may facilitate viral particle proliferation and maturation, but further research is needed to confirm this.…”

Section: Discussionmentioning

confidence: 84%

Comparative Transcriptomic Analysis Revealed Potential Differential Mechanisms of Grass Carp Reovirus Pathogenicity

Kong,

Ding,

Yang

et al. 2023

IJMS

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Grass carp reovirus (GCRV), one of the most serious pathogens threatening grass carp (Ctenopharyngodon idella), can lead to grass carp hemorrhagic disease (GCHD). Currently, GCRV can be divided into three genotypes, but the comparison of their pathogenic mechanisms and the host responses remain unclear. In this study, we utilized the Ctenopharyngodon idella kidney (CIK) model infected with GCRV to conduct comparative studies on the three genotypes. We observed a cytopathic effect (CPE) in the GCRV-I and GCRV-III groups, whereas the GCRV-II group did not show any CPE. Moreover, a consistent trend in the mRNA expression levels of antiviral-related genes across all experimental groups of CIK cells was detected via qPCR and further explored through RNA-seq analysis. Importantly, GO/KEGG enrichment analysis showed that GCRV-I, -II, and -III could all activate the immune response in CIK cells, but GCRV-II induced more intense immune responses. Intriguingly, transcriptomic analysis revealed a widespread down-regulation of metabolism processes such as steroid biosynthesis, butanoate metabolism, and N-Glycan biosynthesis in infected CIK cells. Overall, our results reveal the CIK cells showed unique responses in immunity and metabolism in the three genotypes of GCRV infection. These results provide a theoretical basis for understanding the pathogenesis and prevention and control methods of GCRV.

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Section: Discussionmentioning

confidence: 84%