Identification of lipopeptides from Bacillus strain Q11 with ability to inhibit the germination of Penicillium expansum, the etiological agent of postharvest blue mold disease

Rodríguez‐Chávez, José Luis; Juárez-Campusano, Yara Suhan; Delgado, Guillermo; Aguilar, Juan Ramiro Pacheco

doi:10.1016/j.postharvbio.2019.05.011

Cited by 29 publications

(15 citation statements)

References 43 publications

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“…The production of antimicrobial metabolites by Bacillus is one of the most reported strategies in the antagonistic activity. In our study, the antagonistic strains to P. expansum produced siderophores and lipopeptides, two of the metabolites most reported in Bacillus strains with antifungal activity (Rodríguez-Chávez et al, 2019;Yu et al, 2011). Lipopeptides comprise mainly three families of non-ribosomally synthesized compounds: surfactin, iturin, and fengycin.…”

Section: Discussionmentioning

confidence: 67%

“…The negative control consisted of PDA plates only inoculated with P. expansum, and as positive controls a commercial biological strain Bacillus spp. Q11 (Rodríguez-Chávez et al, 2019) and nystatin fungicide (Shehata et al, 2017) were used. All the plates were incubated at 28°C for 8 days and the mycelial growth diameter was measured, and the antagonistic activity was expressed as the percentage of inhibition of radial growth (PIRG):…”

Section: Molecular Identification Of Bacterial Strainsmentioning

confidence: 99%

“…The bacteria that produced clearing zones around colonial growth were considered positive for surfactant activity (Sarwar et al, 2018). To determine the nature of the produced lipopeptides, the bacterial supernatants were analyzed through the technique of the matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS) and detected in a range of 900 to 1,600 of mass/charge (m/z) (Rodríguez-Chávez et al, 2019;Yang et al, 2015). Siderophore production was determined qualitatively using the Chrome azurol S (CAS) assay.…”

Section: Characterization Of Antifungal Bacterial Compoundsmentioning

confidence: 99%

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Antagonistic Activity of Bacteria Isolated from Apple in Different Fruit Development Stages against Blue Mold Caused by Penicillium expansum

et al. 2021

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Blue mold caused by Penicillium expansum is one of the most significant postharvest diseases of apples. Some microorganisms associated with the surface of ripening apples possess the ability to inhibit the growth of P. expansum. However, the existing literature about their colonization in the stages before ripening is not explored in depth. This study aims to characterize the antagonistic capacity of bacterial populations from five fruit development stages of 'Royal Gala' apples. The results have shown that the density of the bacterial populations decreases throughout the ripening stages of fruit (from 1.0 × 10 5 to 1.1 × 10 1 cfu/cm 2). A total of 25 bacterial morphotypes (corresponding to five genera identified by 16S RNA) were differentiated in which Bacillus stood out as a predominant genus. In the in vitro antagonism tests, 10 Bacillus strains (40%) inhibited the mycelial growth of P. expansum from 30.1% to 60.1%, while in fruit bioassays, the same strains reduced the fruit rot ranging from 12% to 66%. Moreover, the bacterial strains with antagonistic activity increased in the ripening fruit stage. B. subtilis subsp. spiziennii M24 obtained the highest antagonistic activity (66.9% of rot reduction). The matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis revealed that bacteria with antagonistic activity produce antifungal lipopeptides from iturin and fengycin families.

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Section: Discussionmentioning

confidence: 67%

Section: Molecular Identification Of Bacterial Strainsmentioning

confidence: 99%

Section: Characterization Of Antifungal Bacterial Compoundsmentioning

confidence: 99%

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Antagonistic Activity of Bacteria Isolated from Apple in Different Fruit Development Stages against Blue Mold Caused by Penicillium expansum

et al. 2021

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“…Likewise, the antifungal metabolites produced by B. subtilis V26 significantly inhibited the growth of B. cinerea , avoiding the grey mold disease ( Kilani-Feki et al, 2016 ). In another study, the lipopeptide fraction composed of iturin and fengycin produced through B. subtilis inhibited the mycelial growth and conidia germination of funguses like Rhizoctonia solani , Sclerotium rolfsii , Fusarium stilboides, and Penicillium expansum, being causal agents of the postharvest blue mold in apple fruit ( Rodríguez-Chávez et al, 2019 ). More recently, lipopeptide extract produced by the B. velezensis FZ06 showed a positive effect on Gram-positive bacteria at a concentration from 512 to 2048 μg/mL.…”

Section: New Developed Disinfection Methodsmentioning

confidence: 99%

Conventional and non-conventional disinfection methods to prevent microbial contamination in minimally processed fruits and vegetables

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Luna

Pozo

et al. 2022

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“…Lipopeptides (LPs) are the major antifungal compounds produced by Bacillus strains, and they modify cell membrane permeability, induce systemic resistance in plants, and are involved in biofilm formation. The LPs produced by Bacillus strains represent important tools in the development of new effective products against plant pathogens ( Rodríguez-Chávez et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Biocontrol and Action Mechanism of Bacillus subtilis Lipopeptides’ Fengycins Against Alternaria solani in Potato as Assessed by a Transcriptome Analysis

et al. 2022

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Alternaria solani is an airborne fungus and the primary causal agent of potato early blight worldwide. No available fungicides that are both effective and environmentally friendly are usable to control this fungus. Therefore, biological control is a potential approach for its suppression. In this study, Bacillus subtilis strain ZD01’s fermentation broth strongly reduced A. solani pathogenicity under greenhouse conditions. The effects of strain ZD01’s secondary metabolites on A. solani were investigated. The exposure of A. solani hyphae to the supernatant resulted in swelling and swollen sacs, and the ZD01 supernatant reduced A. solani conidial germination significantly. Matrix-assisted laser desorption/ionization time of flight mass spectrometry and pure product tests revealed that fengycins were the main antifungal lipopeptide substances. To elucidate the molecular mechanism of the fengycins’ biological control, RNA sequencing analyses were performed. A transcriptome analysis revealed that 304 and 522 genes in A. solani were differentially expressed after 2-h and 6-h fengycin treatments, respectively. These genes were respectively mapped to 53 and 57 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. In addition, the most enriched KEGG pathway analysis indicated that the inhibitory mechanisms of fengycins against A. solani regulated the expression of genes related to cell wall, cell membrane, transport, energy process, protein synthesis and genetic information. In particular, cell wall and cell membrane metabolism were the main processes affected by fengycin stress. Scanning and transmission electron microscope results revealed hyphal enlargement and a wide range of abnormalities in A. solani cells after exposure to fengycins. Furthermore, fengycins induced chitin synthesis in treated cells, and also caused the capture of cellular fluorescent green labeling and the release of adenosine triphosphate (ATP) from outer membranes of A. solani cells, which may enhance the fengycins ability to alter cell membrane permeability. Thus, this study increases the transcriptome data resources available and supplies a molecular framework for B. subtilis ZD01 inhibition of A. solani HWC-168 through various mechanisms, especially damaging A. solani cell walls and membranes. The transcriptomic insights may lead to an effective control strategy for potato early blight.

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Identification of lipopeptides from Bacillus strain Q11 with ability to inhibit the germination of Penicillium expansum, the etiological agent of postharvest blue mold disease

Cited by 29 publications

References 43 publications

Antagonistic Activity of Bacteria Isolated from Apple in Different Fruit Development Stages against Blue Mold Caused by Penicillium expansum

Antagonistic Activity of Bacteria Isolated from Apple in Different Fruit Development Stages against Blue Mold Caused by Penicillium expansum

Conventional and non-conventional disinfection methods to prevent microbial contamination in minimally processed fruits and vegetables

Biocontrol and Action Mechanism of Bacillus subtilis Lipopeptides’ Fengycins Against Alternaria solani in Potato as Assessed by a Transcriptome Analysis

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