Identification of lipopeptides in Bacillus megaterium by two-step ultrafiltration and LC–ESI–MS/MS

Ma, Yunxiao; Kong, Qing; Qin, Chong; Chen, Yulin; Chen, Yujie; Lv, Ruihuan; Zhou, Guanghui

doi:10.1186/s13568-016-0252-6

Cited by 87 publications

(61 citation statements)

References 21 publications

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“…The production of lipopeptide by Bacillus species is based on the strains: some strains can coproduce two or three classes of lipopeptides, while others can yield only one class [11][12][13]. B. licheniformis MB01 produces surfactin only [12], B. subtilis K1 coproduces surfactins and iturins, while B. amyloliquefaciens SYBC H47 yields three types of lipopeptides, namely, bacillomycin, fengycin and surfactin [46].…”

Section: Discussionmentioning

confidence: 99%

Biocontrol of tomato bacterial wilt by the new strain Bacillus velezensis FJAT-46737 and its lipopeptides

Chen

Wang²,

Liu

et al. 2020

Preprint

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Background: There is an urgent need to discover biological biocontrol agents to control bacterial wilt. This study reports on a new lipopeptide-producing biocontrol strain FJAT-46737 and explores its lipopeptidic compounds, and this study investigates the antagonistic effects of these compounds.Results: Based on a whole genome sequence analysis, the new strain FJAT-46737 was identified as Bacillus velezensis, and seven gene clusters responsible for the synthesis of bioactive secondary metabolites in FJAT-46737 were predicted. The antimicrobial results demonstrated that FJAT-46737 exhibited broad-spectrum antimicrobial activities in vitro against three bacteria and three fungi. Pot experiments showed that the control efficiencies for tomato bacterial wilt of the whole cultures, the 2-fold diluted supernatants and the crude lipopeptide of FJAT-46737 were 66.2%, 82.0%, and 96.2%, respectively. The above results suggested that one of the antagonistic mechanisms of FJAT-46737 was the secretion of lipopeptides consisting of iturins, fengycins and surfactins. The crude lipopeptides had significant antagonistic activities against several pathogens (including Ralstonia solanacearum, Escherichia coli and Fusarium oxysporum) and fengycins were the major antibacterial components of the lipopeptides against R. solanacearum in vitro. Furthermore, the rich organic nitrogen sources (especially yeast extracts) in the media promoted the production of fengycin and surfactin by FJAT-46737. The secretion of these two lipopeptides was related to temperature fluctuations, with the fengycin content decreasing by 96.6% and the surfactins content increasing by 59.9% from 20 oC to 40 oC. The optimal temperature for lipopeptide production by FJAT-46737 varied between 20 oC and 25oC. Conclusions: The B. velezensis strain FJAT-46737 and its secreted lipopeptides could be used as new sources of potential biocontrol agents against several plant pathogens, and especially the bacterial wilt pathogen R. solanacearum.

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Section: Discussionmentioning

confidence: 99%

Biocontrol of tomato bacterial wilt by the new strain Bacillus velezensis FJAT-46737 and its lipopeptides

Chen

Wang²,

Liu

et al. 2020

Preprint

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“…Debido a lo versátil de la cromatografía para el análisis de lipopéptidos -permitiendo su purificación, identificación y cuantificación-esta herramienta analítica ha sido la más utilizada en el procesamiento de lipopéptidos. No obstante, existen otros métodos espectroscópicos que han sido empleados para el análisis de estos compuestos, como i) la resonancia magnética nuclear, del inglés nuclear magnetic resonance (NMR) (Son et al, 2016), transmisión de infrarrojo con transformada de Fourier, del inglés fourier transform infrared spectroscopy (FT-IR) (Kong, 2007) y espectrometría de masas (Ma et al, 2016).…”

Section: Identificación Y Cuantificación De Lipopéptidosunclassified

“…ESI-MS/MS es un método de espectrometría de masas muy completo para hacer identificación de lipopéptidos. Cuando ESI-MS/MS se acopla a HPLC (HPLC-ESI-MS/MS) permite separar, identificar y cuantificar muestras de lipopéptidos de manera simultánea, siendo el instrumento más completo para el análisis de lipopéptidos (Ma et al, 2016). La cuantificación y separación de lipopéptidos con este instrumento tiene el mismo principio que HPLC, ya que esta unidad está acoplada a un espectrómetro de masas.…”

Section: Espectrometría De Masas (Ms)unclassified

Lipopéptidos producidos por agentes de control biológico del género Bacillus: revisión de herramientas analíticas utilizadas para su estudio

Ruiz

Gamboa

Rodríguez³

et al. 2020

Remexca

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El género Bacillus presenta una amplia diversidad metabólica asociada a su prevalencia en diversos ecosistemas. En la actualidad, una de las características más estudiadas de este género bacteriano es su capacidad para disminuir la incidencia de enfermedades en cultivos agrícolas, debido a la represión directa o indirecta del crecimiento de los agentes causales dichas enfermedades. Entre los mecanismos más estudiados en el control biológico de cepas del género Bacillus contra agentes fito-patógenos destacan los lipopéptidos, tales como surfactinas, iturinas y fengicinas, los cuales son compuestos de bajo peso molecular con características anfifílicas que proporcionan protección a las plantas tanto en condiciones previas como posteriores a la cosecha. Los lipopéptidos pueden antagonizar directamente a los agentes fito-patógenos y inducir estrategias de resistencia sistémica en las plantas asociadas. Sin embargo, el amplio uso de lipopéptidos en el sector agrícola, farmacéutico, o alimentario depende del desarrollo de procesos analíticos eficientes y económicos para la extracción, identificación, y cuantificación de dichos compuestos bioactivos. Este ensayo presenta un análisis sobre diversos procedimientos analíticos empleados para la extracción, e identificación cualitativa y cuantitativa de estos metabolitos.

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Section: Velezensismentioning

confidence: 99%

Biocontrol of tomato bacterial wilt by the new strain Bacillus velezensis FJAT-46737 and its lipopeptides

Chen

Wang²,

Liu

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: There is an urgent need to discover alternative Bacillus biocontrol agents to control bacterial wilt. This study reports on a new lipopeptide-producing biocontrol strain FJAT-46737 and explores its lipopeptidic compounds, and this study investigates the antagonistic effects of these compounds.Results: Based on a whole genome sequence analysis, the new strain FJAT-46737 was identified as Bacillus velezensis, and seven gene clusters responsible for the synthesis of bioactive secondary metabolites in FJAT-46737 were predicted. The antimicrobial results demonstrated that FJAT-46737 exhibited broad-spectrum antimicrobial activities in vitro against three bacteria and three fungi. Pot experiments showed that the control efficiencies for tomato bacterial wilt of the whole cultures, the 2-fold diluted supernatants and the crude lipopeptide of FJAT-46737 were 66.2%, 82.0%, and 96.2%, respectively. The above results suggested that one of the antagonistic mechanisms of FJAT-46737 was the secretion of lipopeptides consisting of iturins, fengycins and surfactins. The crude lipopeptides had significant antagonistic activities against several pathogens (including Ralstonia solanacearum, Escherichia coli and Fusarium oxysporum) and fengycins were the major antibacterial components of the lipopeptides against R. solanacearum in vitro. Furthermore, the rich organic nitrogen sources (especially yeast extracts) in the media promoted the production of fengycin and surfactin by FJAT-46737. The secretion of these two lipopeptides was related to temperature fluctuations, with the fengycin content decreasing by 96.6% and the surfactins content increasing by 59.9% from 20 oC to 40 oC. The optimal temperature for lipopeptide production by FJAT-46737 varied between 20 oC and 25oC. Conclusions: The B. velezensis strain FJAT-46737 and its secreted lipopeptides could be used as new sources of potential biocontrol agents against several plant pathogens, and especially the bacterial wilt pathogen R. solanacearum.

show abstract

Identification of lipopeptides in Bacillus megaterium by two-step ultrafiltration and LC–ESI–MS/MS

Cited by 87 publications

References 21 publications

Biocontrol of tomato bacterial wilt by the new strain Bacillus velezensis FJAT-46737 and its lipopeptides

Biocontrol of tomato bacterial wilt by the new strain Bacillus velezensis FJAT-46737 and its lipopeptides

Lipopéptidos producidos por agentes de control biológico del género Bacillus: revisión de herramientas analíticas utilizadas para su estudio

Biocontrol of tomato bacterial wilt by the new strain Bacillus velezensis FJAT-46737 and its lipopeptides

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