Identification of lipopeptides produced by Bacillus subtilis Czk1 isolated from the aerial roots of rubber trees

Abstract

ABSTRACT. We obtained a strain of Bacillus subtilis, which we named Czk1, from the aerial roots of rubber trees. This bacterial isolate exhibits strong antagonistic activity against Ganoderma pseudoferreum, Phellinus noxius, Helicobasidium compactum, Rigidoporus lignosus, Sphaerostilbe repens, and Colletotrichum gloeosporioides. Our earlier research has shown that the antagonistic activity of a fermentation supernatant Czk1 isolate produces a complex mixture of lipopeptides. In this study, we used methanol to extract crude lipopeptides, purified them using a Sephadex G-25 column, cloned the lipopeptide genes, and analyzed purified fractions by matrix-assisted laser desorption ionizationtime of flight mass spectrometry (MALDI-TOF-MS) to identify the lipopeptides from B. subtilis strain Czk1. The cloned lipopeptide genes included those that encode the enzymes lpa, ituD, sfp, and fenB. The crude lipopeptides were purified and found in five fractions. Further analysis revealed that five fractions of the purified composition contained members of the surfactin, iturin, fengycin, and bacillomycin families of antibiotics. This suggests that these lipopeptides from strain Czk1 have potential as plant disease biocontrol agents.

The brown root rot fungus Phellinus noxius affects microbial communities in different root‐associated niches of Ficus trees

“…Trichoderma spp., Bacillus spp., and Streptomyces spp. were shown to have antagonistic activity against P. noxius and could effectively reduce BRR in greenhouse assays (Schwarze et al ., 2012; Ribera et al ., 2016; He et al ., 2017; Shafi et al ., 2017; Chou et al ., 2019; Leung et al ., 2020). Burkholderia cenocepacia 869T2, an endophytic bacterium used to control Fusarium wilt in banana, was observed to temporarily inhibit the growth of P. noxius in dual‐cultures.…”

Characterization of Biopesticide Compounds From Bacillus Subtilis Aaf2 Uaac 20701 Fermentation Products

“…Results above showed that the compound obtained in this research was different from general compound produced by Bacillus subtilis such as surfactine that has weight of 994 -1.065 m/z 3,4,6 iturine which is weighed 1.028 -1.109 m/z 4,5 , bacillomycine that has weight 1081 m/z 6 , fengisine weighed 1.421 -1.566 m/z 3,4,5,6 , and subtilisine which has weight 3.399 -3.473 m/z 4 .…”

“…Bacillus subtilis was reported to have ability to inhibit soil phytopathogen because it has the ability to produce antibiotics, such as lipopeptide antibiotics 1,2 . Antibiotic compounds produced by Bacillus subtilis are surfactine 3,4 , iturine 4,5 , bacillomycine 6 , fengicine 5,6 , and subtilicine 4 . In our previous research, Bacillus subtilis AAF2 was reported to have the ability to inhibit the growth of phytopathogen.…”

Bacillus amyloliquefaciens—Derived Lipopeptide Biosurfactants Inhibit Biofilm Formation and Expression of Biofilm-Related Genes of Staphylococcus aureus

“…The results of these authors showed that PCR products of sfp, srfD, fenB, ituA, and ituC were amplified. He et al [33] reported the presence of the LP genes sfp (surfactin), fenB (fengycin), ituD (iturin) in B. subtilis Czk1, which was obtained from the aerial roots of rubber trees. It was contemplated that the co-production of several LPs by one strain can increase their synergistic effect [34].…”