Identification of &lt;i&gt;Moraxella lacunata&lt;/i&gt; from pulmonary abscesses in three zoo herbivores

Kim, Kyoo-Tae; Lee, Seung-Hun; Kwak, Dongmi

doi:10.1292/jvms.18-0455

“…The findings should be of concern to veterinarians and other public health officers because the members of the Enterobacteriaceae identified have been implicated in hospital-acquired infections in companion animals [4, 30–32]. Similarly, the non-enteric Gram-negative bacilli isolated in this study are important animal pathogens and have been reported in many disease conditions, such as wooden tongue in cattle, fatal pneumonia in pigs and septicaemia in neonatal foal caused by Actinobacillus species [33], infectious bovine keratoconjunctivitis [34, 35] and pulmonary abscesses in zoo herbivores [36] caused by Moraxella species.…”

Section: Discussionmentioning

confidence: 94%

Veterinary clinic surfaces as reservoirs of multi-drug- and biocide-resistant Gram-negative bacteria

Akwuobu

¹

,

Ngbede

²

,

Mamfe

³

et al. 2021

Access Microbiology

1

0

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This cross-sectional study was carried out to determine the common Gram-negative bacteria (GNB) contaminating veterinary clinic environments, and to evaluate the susceptibility of the isolates to commonly used antibiotics and biocides. A total of 62 swab samples were collected from different frequently touched surfaces in the 4 veterinary clinics visited. The samples were processed for isolation and identification of GNB using standard microbiological procedures. The susceptibility of the isolates to disinfectants and antibiotics was determined using agar dilution and disc diffusion techniques, respectively. A total of 114 GNB were isolated from the 4 clinics with isolation rates of 21.9, 22.8, 23.7 and 31.6% in clinics A, B, C and D, respectively. The surfaces of treatment tables were more contaminated (16.7 %) than receptionist/clinician desks (15.8%), weighing balances (10.5 %), door handles (7.9 %), drip stands (7.9 %), handwashing basins (7.0 %) and client chairs (7.0%). The surface-contaminating isolates were distributed into 20 genera, with members of Enterobacteriaceae predominating (n=97). Fifty-nine per cent of the isolates were resistant to the disinfectant Septol, while 5.3 and 0.9% were resistant to Purit and Dettol disinfectants, respectively. Multiple drug resistance was observed among 99% of the isolates with approximately 100% resistance to beta-lactams. Phenotypic expression of extended-spectrum (3.5 %) and AmpC beta-lactamase (38.6 %) production was detected. These findings highlight the role of clinic environments in serving as reservoirs for potential pathogens and sources for the spread of multi-drug resistant GNB.

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“…Some Moraxella species, such as Moraxella catarrhalis , Moraxella nonliquefaciens and Moraxella osloensis , are important common pathogens of human [18–20]. Moreover, A few Moraxella species, such as Moraxella bovoculi , Moraxella ovis and Moraxella lacunata , are rare pathogens of animals [21–23].…”

Section: Full-textmentioning

confidence: 99%

Moraxella nasovis sp. nov., isolated from a sheep with respiratory disease

Li

¹

,

Zhu

²

,

Li

³

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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A novel Gram-stain-negative, aerobic, coccus-shaped bacteria, designated ZY201115T, was isolated from the nasal cavity of a sheep with respiratory disease in Yunnan Province, south-west China, and its taxonomic affiliation was studied by applying a polyphasic approach. The strain grew at 18–41 °C (optimum, 37 °C), at pH 6.0–9.0 (optimum, pH 8.0) and in 0.5–3.0% (w/v) NaCl (optimum, 1.0 % NaCl). Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain is affiliated to the genus Moraxella with highest similarity to Moraxella bovis ATCC 10900T (96.6 %). Phylogenomic analysis based on 811 single-copy genes also indicated that the strain represents a novel species in the genus Moraxella and formed a deep and separated clade with Moraxella caviae NCTC 10293T. The highest genomic orthologous average nucleotide identity and digital DNA–DNA hybridization values between the strain and the type strains in the genus Moraxella were 73.7% ( M. caviae NCTC 10293T) and 25.3% ( Moraxella osloensis CCUG 350T), respectively. The G+C content of the complete genome sequence was 42.1 mol%. The predominant fatty acids (>5 %) were C18:1 ω9c, C17:1 ω8c, C12:03OH and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c). The major polar lipids were phosphatidylglycerol, cardiolipin, monolysocardiolipin, phosphatidylethanolamine and hemibismonoacylglycerophosphate. The major respiratory quinone was CoQ-8. On the basis of the results of phylogenetic, phenotypic and chemotaxonomic characterizations, strain ZY201115T clearly represents a novel species of the genus Moraxella , for which the name Moraxella nasovis sp. nov. is proposed. The type strain is ZY201115T (=CCTCC AB 2021473T=CCUG 75922T).

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Moraxella

Fernández‐Garayzábal¹,

Miguela‐Villoldo²,

Vela³

2022

Bergey's Manual of Systematics of Archaea and Bacteria

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Mo.ra.xel'la. N.L. dim. fem. n. Moraxella , named after V. Morax, a Swiss ophthalmologist who pioneered the recognition of the type species. Proteobacteria / Gammaproteobacteria / Pseudomonadales / Moraxellaceae / Moraxella Moraxella species are Gram‐negative rods or cocci but often with a tendency to resist decolorization. The rods are often very short and plump, frequently approaching a coccus shape (1.0–1.5 × 1.5–1.5 μm); they usually occur in pairs and short chains. Variation in cell size, shape, and filament or chain formation is often seen in cultures, the pleomorphism being enhanced by lack of oxygen and by incubation temperatures above the optimum. The cocci are usually smaller (0.6–1.0 μm in diameter) and occur as single cells or in pairs; sometimes results in the formation of tetrads. Flagella are absent. Both coccal and rod‐shaped species can be fimbriated. Absence of swimming motility, but surface‐bound “twitching motility” has been observed in some rod‐shaped species. Aerobic, but some species can grow weakly under anaerobic conditions. May be encapsulated. Chemoorganotrophic. Most species are nutritionally fastidious, and all grow in complex media; some are stimulated significantly by fatty acids (bile salts and Tween 80). The optimal temperature for growth is between 33 and 35°C. Colonies are not pigmented. Oxidase‐positive. Usually catalase‐positive. No acid is produced from carbohydrates. Species of this genus share 96–99% similarity in their 16S rRNA gene sequences. The major fatty acids in most species are C 18 :1 ω9 c and C 16:1 ω7 c . The major quinone is Q‐8. Members of the class Gammaproteobacteria and family Moraxellaceae . Parasitic on the mucous membranes of humans and other warm‐blooded animals. DNA G + C content (mol%) : 40–48.1 ( T m , Bd, HPLC). Type species : Moraxella lacunata Lwoff 1939 AL , synonym: “ Bacillus lacunatus ” Eyre 1900.

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Identification of <i>Moraxella lacunata</i> from pulmonary abscesses in three zoo herbivores

Cited by 8 publications

References 15 publications

Veterinary clinic surfaces as reservoirs of multi-drug- and biocide-resistant Gram-negative bacteria

Veterinary clinic surfaces as reservoirs of multi-drug- and biocide-resistant Gram-negative bacteria

Moraxella nasovis sp. nov., isolated from a sheep with respiratory disease

Moraxella

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