Identification of marker compounds for discriminating between embryogenic and nonembryogenic calluses of higher plants using pyrolysis gas chromatography mass spectrometry and genetic programming

Kim, Suk Weon; Ban, Sung Hee; Yoo, Ook Joon; Liu, Jang Ryol

doi:10.1007/bf02931866

Cited by 4 publications

(4 citation statements)

References 18 publications

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“…In our current study, we demonstrated that an extensive number of cultivars from a given species can be separated via DA and that a hierarchical dendrogram based on DA can describe possible relationships among cultivars that are equivalent to the genetic relationships revealed by DNA-fingerprinting methods. We have also shown earlier that PyMS combined with multivariate analysis of the data allows us to distinguish embryogenic from non-embryogenic calli in various higher plants (Kim et al 2006(Kim et al , 2007b.…”

Section: Discussionmentioning

confidence: 90%

Genetic Discrimination of Catharanthus roseus Cultivars by Pyrolysis Mass Spectrometry

Kim

Liu

2009

J. Plant Biol.

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Section: Discussionmentioning

confidence: 90%

Genetic Discrimination of Catharanthus roseus Cultivars by Pyrolysis Mass Spectrometry

Kim

Liu

2009

J. Plant Biol.

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“…An effort is underway to identify the chemical compounds by pyrolysis gas chromatography-mass spectrometry (Kim et al 2006). PyMS analysis has been widely used to discriminate closely related microbial strains (Freeman et al 1994;Goodacre et al 1994Goodacre et al , 1996a.…”

Section: 100mentioning

confidence: 99%

Discrimination of higher plant calluses based on embryogenic capacity and taxonomic classification by pyrolysis mass spectrometry

Kim

Ban

Yoo

et al. 2007

Plant Biotechnol Rep

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Pyrolysis mass spectrometry (PyMS) is a rapid, simple, high-resolution analytical method based on thermal degradation of complex material in a vacuum, and has been widely applied to the discrimination of closely related microbial strains. Minimally prepared samples of embryogenic and non-embryogenic calluses derived from various higher plants (sweet potato, morning glory, Korean ginseng, Siberian ginseng, and balloon flower) were subjected to PyMS for spectral fingerprinting. A dendrogram based on the unweighted pair group method, with arithmetic mean of pyrolysis mass spectra, divided the calluses into Siberian ginseng embryogenic callus and the others, which were subsequently divided into embryogenic and nonembryogenic callus groups, regardless of plant species from which the calluses were derived. In the nonembryogenic callus group, the dendrogram was in agreement with the known taxonomy of the plants.These results indicate that PyMS analysis could be applied for discriminating plant calluses based on embryogenic capacity and taxonomic classification.

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“…Metabolite characterization has been applied to various biotechnological fields, including monitoring of biological processes and identification of “marker” compounds. In particular, it was possible to discriminate between embryogenic and nonembryogenic calli of some higher plants Catharanthus roseus cultivars .…”

Section: Introductionmentioning

confidence: 99%

“…In particular, it was possible to discriminate between embryogenic and nonembryogenic calli of some higher plants. 33 1 H NMR-based metabolomic analysis was found to be effective for genetic discrimination of Catharanthus roseus cultivars. 34 Moreover, the technique allowed the metabolic discrimination of callus cells of the same species according to their relative locations and was suggested as an objective criterion to previously select callus portions to be transferred to liquid culture, aiming to enhance the production of useful secondary metabolites.…”

Section: ■ Introductionmentioning

confidence: 99%

Effect of a Peat Humic Acid on Morphogenesis in Leaf Explants of Pyrus communis and Cydonia oblonga. Metabolomic Analysis at an Early Stage of Regeneration

Marino

Righi

Simoni

et al. 2013

J. Agric. Food Chem.

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Plant regeneration is a critical step in most in vitro breeding techniques. This paper studies the effects of a low-molecular-weight humic acid (HA) on morphogenesis from pear and quince leaf explants. Variable HA amounts [0 (control), 1, 5, 10, and 20 mg C L(-1)] were added to the regeneration media. A dose-response effect was observed in pear for root and shoot production; it was improved at HA 1 mg C L(-1) and considerably reduced at the highest amounts. HA was, instead, ineffective in quince. The (1)H HR-MAS NMR analyses of calli in the induction phase showed more evident metabolite (asparagine, alanine, and γ-aminobutyric acid) signals in quince than in pear. The assignment of overlapped signals in both genotypes was supported by the 2D NMR analyses. Spectroscopic characterization suggested also an enhancement of asparagine contents in morphogenic calli of pear with respect to the control and higher HA amount treatments.

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Identification of marker compounds for discriminating between embryogenic and nonembryogenic calluses of higher plants using pyrolysis gas chromatography mass spectrometry and genetic programming

Cited by 4 publications

References 18 publications

Genetic Discrimination of Catharanthus roseus Cultivars by Pyrolysis Mass Spectrometry

Genetic Discrimination of Catharanthus roseus Cultivars by Pyrolysis Mass Spectrometry

Discrimination of higher plant calluses based on embryogenic capacity and taxonomic classification by pyrolysis mass spectrometry

Effect of a Peat Humic Acid on Morphogenesis in Leaf Explants of Pyrus communis and Cydonia oblonga. Metabolomic Analysis at an Early Stage of Regeneration

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