Identiﬁcation of mcr-10 carried by self-transmissible plasmids and chromosome in Enterobacter roggenkampii strains isolated from hospital sewage water

Xu, Tingting; Zhang, Chuqiu; Ji, Yang; Song, Jingjie; Yang, Liu; Guo, Yuqi; Zhou, Kai

doi:10.1016/j.envpol.2020.115706

Cited by 82 publications

(77 citation statements)

References 43 publications

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“…So far, there have been ten variants ranging from mcr-1 to mcr-10 [13][14][15]. What is worrying is that antibiotic-resistant superbugs are prevalent in the entire world.…”

Section: Introductionmentioning

confidence: 99%

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Co-infections of two carbapenemase-producing Enterobacter hormaechei clinical strains isolated from the same diabetes individual in China

Ding

Shi

Din

et al. 2021

Journal of Medical Microbiology

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Introduction. Since mcr-1 was first reported in China, there have been ten variants of MCR appearing nationwide so far. Multidrug-resistant Enterobacteriaceae bacteria carrying both NDM and MCR have become a serious threat to global public health. Hypothesis/Gap Statement. The genetic structure of mcr-9 needs to be better understood in order to better prevent and control the transmission of drug-resistant genes. Aims. The aim of this study was to characterize the presence of two Enterobacter hormaechei isolates, which carries bla NDM-5 CME2 and the coexistence of mcr-9 and bla NDM-1 strain CMD2, which were isolated from a patient with diabetes in Sichuan, China. Methodology. The microbroth dilution method was used for antibiotic susceptibility. Conjugation experiment was used to investigate the transferability of bla NDM-1, bla NDM-5 and mcr-9. Whole-genome sequencing was performed on Illumina HiSeq platform. The ability of biofilm formation was detected by crystal-violet staining, the virulence of the bacteria was measured by Galleria mellonella killing assay. Results. bla NDM-5 carrier CME2 and CMD2 with bla NDM-1 and mcr-9 were resistant to carbapenems, β-lactam, aminoglycoside, quinolone and tetracycline, while CMD2 was also resistant to colistin. Conjugation assay and plasmid replicon typing further demonstrated that both bla NDM-1 and bla NDM-5 were respectively present on the self-transferrable IncX3 plasmid, mcr-9 was located on the self-transferrable IncHI2 plasmid. Through the analysis of mcr-9 gene context, the structure was DUF4942-rcnR-rcnA-copS-IS903-mcr-9-wbuC-qseC-qseB-IS1R-ΔsilR-IS903, bla NDM-1 context was IS3000-ΔISAba125-IS5-bla NDM-1-ble-trpF-groS-groL-insE-ΔIS26 structure, bla NDM-5 structure was IS3000-bla NDM-5-ble-trpF-dsbC-ΔIS26-umuD-ISKox3-tnpR-parA. Biofilm formation of CME2 was stronger than CMD2. There was no significant difference in virulence between the two strains. Conclusion. This study reveals two multiple drug-resistant E. hormaechei isolates from diabetes patient samples. E. hormaechei carrying two NDM-resistant genes is already a serious threat, where MCR is an important cause of treatment failure in bacterial infections. This study is a reminder not only to prevent infection in patients with diabetes, but also to constantly monitor the epidemic and spread of the drug-resistant gene.

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“…So far, there have been ten variants ranging from mcr-1 to mcr-10 [13][14][15]. What is worrying is that antibiotic-resistant superbugs are prevalent in the entire world.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, multidrug-resistant Enterobacteriaceae bacteria carrying both NDM and MCR have been isolated in multiple locations [11,12]. So far, there have been ten variants ranging from mcr-1 to mcr-10 [13][14][15]. What is worrying is that antibiotic-resistant superbugs are prevalent in the entire world.…”

Section: Introductionmentioning

confidence: 99%

Co-infections of two carbapenemase-producing Enterobacter hormaechei clinical strains isolated from the same diabetes individual in China

Ding

Shi

Din

et al. 2021

Journal of Medical Microbiology

View full text Add to dashboard Cite

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“…A plasmid carrying mcr-1 conferring colistin resistance was first identified in China in 2014 (Liu et al, 2016). To date, up to 10 types of the mcr gene family harbored in Enterobacteriaceae have been reported in clinical samples and in animals and human carriers (Xu et al, 2021). Colistin has been extensively used in pig production, often as a growth promoter until the 2017 prohibition of its use under regional policy.…”

Section: Introductionmentioning

confidence: 99%

Reducing the Risk of Transmission of Critical Antimicrobial Resistance Determinants From Contaminated Pork Products to Humans in South-East Asia

et al. 2021

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Antimicrobial resistance (AMR) is a critical challenge worldwide as it impacts public health, especially via contamination in the food chain and in healthcare-associated infections. In relation to farming, the systems used, waste management on farms, and the production line process are all determinants reflecting the risk of AMR emergence and rate of contamination of foodstuffs. This review focuses on South East Asia (SEA), which contains diverse regions covering 11 countries, each having different levels of development, customs, laws, and regulations. Routinely, here as elsewhere antimicrobials are still used for three indications: therapy, prevention, and growth promotion, and these are the fundamental drivers of AMR development and persistence. The accuracy of detection of antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARG) depends on the laboratory standards applicable in the various institutes and countries, and this affects the consistency of regional data. Enterobacteriaceae such as Escherichia coli and Klebsiella pneumoniae are the standard proxy species used for indicating AMR-associated nosocomial infections and healthcare-associated infections. Pig feces and wastewater have been suspected as one of the hotspots for spread and circulation of ARB and ARG. As part of AMR surveillance in a One Health approach, clonal typing is used to identify bacterial clonal transmission from the production process to consumers and patients – although to date there have been few published definitive studies about this in SEA. Various alternatives to antibiotics are available to reduce antibiotic use on farms. Certain of these alternatives together with improved disease prevention methods are essential tools to reduce antimicrobial usage in swine farms and to support global policy. This review highlights evidence for potential transfer of resistant bacteria from food animals to humans, and awareness and understanding of AMR through a description of the occurrence of AMR in pig farm food chains under SEA management systems. The latter includes a description of standard pig farming practices, detection of AMR and clonal analysis of bacteria, and AMR in the food chain and associated environments. Finally, the possibility of using alternatives to antibiotics and improving policies for future strategies in combating AMR in a SEA context are outlined.

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“…A colistin‐resistant isolate HSW1412 was recovered from sewage water samples of a tertiary hospital in October 2019 as described previously (Xu et al ., 2020). HSW1412 was initially identified as the member of E. cloacae complex (ECC) using the Vitek MS (bioMérieux, Marcy‐l'Étoile, France).…”

Section: Figurementioning

confidence: 99%

Identiﬁcation of mcr-10 carried by self-transmissible plasmids and chromosome in Enterobacter roggenkampii strains isolated from hospital sewage water

Cited by 82 publications

References 43 publications

Co-infections of two carbapenemase-producing Enterobacter hormaechei clinical strains isolated from the same diabetes individual in China

Co-infections of two carbapenemase-producing Enterobacter hormaechei clinical strains isolated from the same diabetes individual in China

Reducing the Risk of Transmission of Critical Antimicrobial Resistance Determinants From Contaminated Pork Products to Humans in South-East Asia

A novel host of MCR‐5 belonging to Enterobacter spp. isolated from hospital sewage water

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